Histomorphological structure of the palate and histochemical profiles of the salivary palatine glands in the Chukar partridge (Alectoris chukar, Gray 1830)

Abstract

Morphology of the palatine mucosa and its secretion was described in Chukar partridges, by gross morphology and histochemistry techniques. For this purpose, 10 healthy adults (five male and five female) were sacrificed. After sacrifice, the palatine tissues were extirpated and fixed in 10% formol‐alcohol for 18 h and were embedded longitudinally and transversally in paraffin. The 5‐μm sections were employed histological and histochemical staining techniques. The lateral rims of the caudal part of the choanal cleft were bordered by large conical papillae. In the periphery of the choanal and the infundibular cleft, small papillae were scattered across the palatine mucosa. The palate was lined by keratinized stratified squamous epithelium, which contained conical papillae of varying height. However, the folds of the keratinized stratified squamous epithelial layer covering the choanal and infundibular cleft were nonkeratinized. The rostral aspect of the choanal cleft contained simple branched tubulo‐alveolar glands of both mucous and sero‐mucous characteristic, whilst the caudal aspect included mucous simple branched tubular glands. Furthermore, it was ascertained that the secretion of the palatine glands contained glycoproteins, carboxylated proteoglycans, weakly and strongly sulphated mucins, sialic acid and hyaluronic acid, but lacked glycogen. In conclusion, it was demonstrated that the histological structure of the mucosal epithelium and the supporting elements displayed similarity to those of other domestic avian species.

Papillary Architecture and Functional Characterization of Mucosubstances in the Sheep Tongue

“…For this purpose, we supported the morphological results with conventional mucin histochemistry and immunohistochemistry techniques. Since histochemistry is an excellent method to identify the characterization of glycoconjugates (Schumacher et al, ; Sağsöz and Liman, ; Erdoğan et al, ; Sağsöz et al, ; Erdoğan et al, ), we demonstrated changes in carbohydrate lateral chains of secretions of epithelial cells of the lingual glands. We also evaluated the expression of proteins of MUC1, MUC2, MUC5AC, and MUC6 in epithelial cells of the lingual glands and compared them to the molecular profiles of the mucins.…”

“…The results of carbohydrate histochemistry and MUCs (MUC1, MUC2, MUC5AC, and MUC6) were evaluated as follows: no staining (–), weak staining (+), moderate staining (++), and intense staining (+++) (Buisine et al, ; Schumacher et al, ; Erdogan et al, 2012; Sağsöz et al, ; Erdogan et al, 2015). The evaluations of the positive stained cells were carried out by the same two blind researchers (H.S.…”

Mucin profiles of the abomasum in bulls and rams: A comparative study

“…The results of carbohydrate histochemistry were evaluated as no staining (−), weak staining (+), moderate staining (++), and intense staining (+++) (Erdoğan et al, ; Sağsöz et al, ; Schumacher et al, ). The immunoreactivity for MUC1, MUC2, MUC5AC, and MUC6 was evaluated by a semiquantitative scoring method based on the intensity of staining.…”

“…Acidic mucins are further classified as sulfated mucins (sulfomucins) and carboxylated mucins (sialomucins). While sulfated acidic mucins contain sulfated glucuronic acid, carboxylated acidic mucins contain sialic acid molecules (Erdoğan, Sağsöz, & Akbalık, ; Sağsöz & Liman, ; Sağsöz, Erdoğan, & Akbalik, ; Schumacher, Duku, Katoh, Jörns, & Krause, ). From a molecular perspective, all mucins contain a centrally located domain comprising numerous oligosaccharide chains.…”

Anatomical and scanning electron microscopic characteristics of the oropharyngeal cavity (tongue, palate and laryngeal entrance) in the southern lapwing (Charadriidae: Vanellus chilensis, Molina 1782)

“…Our literature review indicates that very few microscopic evaluations were particularly on the detailed morphological aspects of the palate and laryngeal region (Erdogan and Alan ; Sağsöz et al . , ).…”