Histomorphological and Morphometric Evaluation of Microvessel Density in Nodal Non-Hodgkin Lymphoma Using CD34 and CD105

Sucheta, .; Kataria, Sant Prakash; Malik, Shivani; Yadav, R. K.; Kapil, Raman; Sen, Ruchira

doi:10.1055/s-0041-1726569

Cited by 4 publications

(4 citation statements)

References 22 publications

(43 reference statements)

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“…We further observed that the restored blood vessels particularly densified and widened in two drug-treated groups, marking the activation of an autoimmune response to recruit pre-existing microvessels and produce neocapillaries ( 43 ). These results were verified again with increased vessel density counts reflected in the CD34 expression in our study, as CD34 is a specific endothelial cell marker for neovascularization ( 35 ). The perfused microcirculation network provides greater tissue perfusion, which satisfies the demand for oxygen delivery and nutritional supply for ulcer reconstruction ( 4 ).…”

Section: Discussionsupporting

confidence: 87%

“…Some sections for immunohistochemical staining were excluded from the study, as they did not contain ulcer tissue or tissue after ulcer healing. The microvessel density was then assessed through CD34 staining following a previously described protocol ( 35 ). This was followed by hematoxylin and eosin (HE) staining, based on which an inflammation score from 0 to 4 ( 36 ) was determined by two pathologists in a blinded manner.…”

Section: Methodsmentioning

confidence: 99%

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Dynamic characterization of vascular response and treatment in oral traumatic ulcer in mice via photoacoustic imaging

Miao,

Ma,

et al. 2024

Quant Imaging Med Surg

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Background Dynamic surveillance of vasculature is essential for evaluating the healing of oral ulcer. Existing techniques used in vascular imaging face limitations, such as inadequate spatial resolution, restricted diagnostic depth, and the necessity of exogenous contrast agents. Therefore, this study aimed to use robust photoacoustic imaging (PAI) for the dynamic monitoring of vascular response during healing and the associated treatment process of oral ulcer. Methods Kunming mice (male, 8 weeks old, 31–41 g) were treated with 50% acetic acid for 90 s on the tongue mucosa for induction of oral traumatic ulcer. Mice were randomly divided into three groups (n=12): the control, compound chamomile and lidocaine hydrochloride gel (CCLH), and phycocyanin (PC) groups. PAI was then conducted on days 0, 2, 3, 5, and 7 to obtain vessel parameters of the ulcer area, including vessel intensity, density, mean diameter, maximum diameter, and curvature. Immunohistochemical and hematoxylin and eosin (HE) staining were performed on days 3 and 7 to assess microvessel density and inflammation score. The ulcer healing rate and body weight changes were evaluated for clinical observation. Results Beginning on the second day after ulcer induction, there was a progressive increase over time in blood intensity and vessel parameters, including vascular density and diameter. On day 7, the CCLH and PC groups demonstrated significantly higher measures than did the control group in terms of blood intensity (P<0.05 and P<0.01), vascular density (both P values <0.05), mean diameter (both P values <0.01), and maximum diameter (P<0.01 and P<0.05). Vessel curvature in the two treatment groups exhibited no significant differences compared to that in the control group (both P values >0.05). The effects of vascular morphological changes were further supported by the histological and clinical outcomes. On day 7, compared to that of the control group, the level of microvessel density was significantly higher in both the CCLH (P<0.01) and PC (P<0.05) groups. The histopathological score in PC group was significantly lower than that of the control group on day 7 (P<0.05). Additionally, compared to that of the control group, the healing rates of the CCLH (P<0.01) and PC groups (P<0.05) were superior on day 7. On day 3, the control group showed more weight loss than did the CCLH (P<0.05) and PC (P<0.01) groups. Conclusions These findings indicate that PAI is a valuable strategy for the dynamic and quantitative analysis of vascular alterations in oral traumatic ulcers and support its prospective application in improving clinical treatment.

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Section: Discussionsupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Dynamic characterization of vascular response and treatment in oral traumatic ulcer in mice via photoacoustic imaging

Miao,

Ma,

et al. 2024

Quant Imaging Med Surg

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“…CD105, better known as endoglin, is a homodimeric transmembrane glycoprotein that highly expressed on activated angiogenic endothelial cells (141). Used as a marker of MVD, CD105 has proven itself as a potent prognostic indicator and a potential target for antiangiogenic therapy and chimeric antigen receptor-based T-cell (CAR-T) immunotherapy in several tumors (142)(143)(144).…”

Section: Proteins In Tumor Tissuementioning

confidence: 99%

Potential Molecular Biomarkers of Vestibular Schwannoma Growth: Progress and Prospects

et al. 2021

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Vestibular schwannomas (VSs, also known as acoustic neuromas) are relatively rare benign brain tumors stem from the Schwann cells of the eighth cranial nerve. Tumor growth is the paramount factor for neurosurgeons to decide whether to choose aggressive treatment approach or careful follow-up with regular magnetic resonance imaging (MRI), as surgery and radiation can introduce significant trauma and affect neurological function, while tumor enlargement during long-term follow-up will compress the adjacent nerves and tissues, causing progressive hearing loss, tinnitus and vertigo. Recently, with the deepening research of VS biology, some proteins that regulate merlin conformation changes, inflammatory cytokines, miRNAs, tissue proteins and cerebrospinal fluid (CSF) components have been proposed to be closely related to tumor volume increase. In this review, we discuss advances in the study of biomarkers that associated with VS growth, providing a reference for exploring the growth course of VS and determining the optimal treatment strategy for each patient.

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“…There is growing evidence of a correlation between the type of vascular condition and the aggressiveness of lymphomas [7,22,23]. To identify signs of transformation and to identify potential biopsy sites, this retrospective study was designed to clarify invasive and indolent Lymphomatous lymph nodes through Ultrasonographic Vascular imaging, which nally impact the selection of treatment programmes.…”

Section: Discussionmentioning

confidence: 99%

Identification of superficial invasive and indolent lymphomatous lymph nodes by multiple Ultrasonographic vascular imaging

Lu,

Li,

Deng

et al. 2024

Preprint

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Objective This study aimed to explore whether superficial invasive lymphomas and indolent lymphomas could be identified by Ultrasonographic vascular imaging. Method A retrospectively study enrolled 82 lymphoma patients. According to proliferation rates and clinical course, the lymph nodes were classified as invasive and indolent lymphomatous lymph nodes. All patients underwent ultrasound (US) with three effective techniques: color Doppler flow imaging (CDFI), angio plus ultrasound imaging (AngioPLUS), and contrast-enhanced ultrasound (CEUS). Qualitative and quantitative parameters from the two groups were compared. Finally, the area under the receiver-operating characteristic (ROC) and regression analysis were used to compare the differences between the two groups and determine the diagnostic efficiency of the three techniques for differentiating invasive lymphoma from indolent lymphoma. Result The types of blood flow distribution between invasive and indolent lymphomatous lymph nodes were statistically different in all three Ultrasound techniques. In CDFI, invasive or indolent lymphomatous lymph nodes were determined by resistance index (RI) (p < 0.001). In CEUS, the differences between the two groups in necrosis and arrival time (ATM) (p = 0.026, 0.043) were statistically significant. Finally, CDFI combined with CEUS had the highest diagnostic sensitivity of 98.1%. Interobserver agreements for qualitative parameters were all excellent. Conclusion Ultrasonographic Vascular imaging is an aid in identifying invasive and indolent lymphomatous lymph nodes, and CDFI combined with CEUS had the highest diagnostic sensitivity, which can guide clinicians to make more accurate diagnosis and better treatment for patients.

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Histomorphological and Morphometric Evaluation of Microvessel Density in Nodal Non-Hodgkin Lymphoma Using CD34 and CD105

Cited by 4 publications

References 22 publications

Dynamic characterization of vascular response and treatment in oral traumatic ulcer in mice via photoacoustic imaging

Dynamic characterization of vascular response and treatment in oral traumatic ulcer in mice via photoacoustic imaging

Potential Molecular Biomarkers of Vestibular Schwannoma Growth: Progress and Prospects

Identification of superficial invasive and indolent lymphomatous lymph nodes by multiple Ultrasonographic vascular imaging

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