Histology of the healing tympanic membrane following perforation in rats

Maria, Peter L. Santa; Redmond, Sharon L.; Atlas, Marcus D.; Ghassemifar, Reza

doi:10.1002/lary.20998

Cited by 78 publications

(57 citation statements)

References 25 publications

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“…Laboratory created acute perforations heal spontaneously and devalues any intervention used in such a model. (17,20). The model used in this study has been validated as mimicking the human condition in histological appearance, making it useful for testing potential treatments for chronic TM perforations.…”

Section: Discussionmentioning

confidence: 99%

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Heparin Binding Epidermal Growth Factor-Like Growth Factor Heals Chronic Tympanic Membrane Perforations With Advantage Over Fibroblast Growth Factor 2 and Epidermal Growth Factor in an Animal Model

Maria

Weierich²,

Kim³

et al. 2015

Otology &Amp; Neurotology

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Hypothesis That heparin binding epidermal growth factor like growth factor (HB-EGF) heals chronic tympanic membrane (TM) perforations at higher rates than fibroblast growth factor 2 (FGF2) and epidermal growth factor (EGF) in an animal model. Background A non-surgical treatment for chronic TM perforation would benefit those unable to access surgery or those unable to have surgery, as well as reducing the cost of tympanoplasty. Growth factor (GF) treatments have been reported in the literature with variable success with the lack of a suitable animal providing a major obstacle. Methods The GFs were tested in a validated mouse model of chronic TM perforation. A bio absorbable hydrogel polymer was used to deliver the GF at a steady concentration as it dissolved over four weeks. A control (polymer only, n=18) was compared to polymer loaded with HB-EGF (5ug/ml, n=18), FGF2 (100ug/ml, n=19) and EGF (250ug/ml, n=19). Perforations were inspected at four weeks. Results The healing rates, as defined as one hundred percent perforation closure, were control (5/18, 27.8%), HB-EGF (15/18, 83.3%), FGF2 (6/19, 31.6%) and EGF (3/19, 15.8%). There were no differences between FGF2 (p=0.80) and EGF (p=0.31) with control healing rates. HB-EGF (p= 0.000001) showed a significant difference for healing. The HB-EGF healed TMs showed layers similar to a normal TM, whilst the other groups showed a lack of epithelial migration. Conclusion This study confirms the advantage of HB-EGF over two other commonly used growth factors and is a promising non-surgical treatment of chronic TM perforations.

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Section: Discussionmentioning

confidence: 99%

“…(16,17) The other half of perforations were subjected to cytokeratin staining described below. Briefly in addition, due to the fragility of the mouse TM, a section of the tympanic bulla was harvested around the TM under microscopic assistance.…”

Section: Methodsmentioning

confidence: 99%

Heparin Binding Epidermal Growth Factor-Like Growth Factor Heals Chronic Tympanic Membrane Perforations With Advantage Over Fibroblast Growth Factor 2 and Epidermal Growth Factor in an Animal Model

Maria

Weierich²,

Kim³

et al. 2015

Otology &Amp; Neurotology

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“…The differences between genotypes were not assessed. A histological account of the wound has been previously published 27…”

Section: Discussionmentioning

confidence: 99%

Tympanic membrane wound healing in rats assessed by transcriptome profiling

et al. 2011

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“…Healing of the fibrous layer occurs secondarily. Finally, the inner epithelium regenerates [1][2][3][4]. There is support for the notion, that healing of TM involves several biological processes such as epithelial proliferation and migration, fibroblasts' proliferation, neoangiogenesis, extracellular matrix (ECM) formation and remodeling [5].…”

Section: Introductionmentioning

confidence: 99%