HISTOLOGY OF THE DUCTUS EPIDIDYMIDIS IN THE DOG (Canis familiaris, L.)

Schimming, Bruno César; Vicentini, Carlos Alberto; Orsi, Antônio Marcos; Franceschini-Vicentini, Irene Bastos; Abreu-Rays, M. A.

doi:10.4067/s0716-98681997000100001

Cited by 9 publications

(7 citation statements)

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“…Basal and apical cells were observed along the epididymis, although apical cells seemed to have the least number in the cauda epididymidis , which may be due to the lower epithelial height in this region (Schimming et al. ). In rodents, apical cells have been found in the proximal epididymal regions, mainly in the IS (Robaire and Hermo ).…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization of Aquaporins 1 and 9 in the Ram Efferent Ducts and Epididymis

Schimming

Pinheiro

Matteis

et al. 2015

Reprod Domestic Animals

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ContentsAquaporins (AQPs) are essential membrane protein channels for the transport of water across membranes. Fluid movement in the epididymis is important for modulation of the luminal environment, in which sperm mature and reside. This study was designed to understand the morphology and localization of AQPs in ram efferent ducts (ED) and epididymis. For this purpose, the epididymis of seven animals were removed for histologic and immunohistochemical analyses. AQP1 immunoreactivity was observed in the apex of the ED, and AQP9 was found adjacent to the nuclei of the epithelial cells of the ED. The epithelial lining of ram epididymis is pseudostratified columnar and presents principal, basal, apical and narrow cells. In the initial segment (IS), a moderate reaction for AQP1 was observed in the apical cytoplasm of epithelial cells. An intense reactivity for AQP1 was noted over the microvilli of principal cells and in spermatozoa in the caput. In the corpus and cauda, AQP1 was noted only over the endothelial cells of vascular channels located in intertubular spaces. A weak-tomoderate reaction for AQP9 was observed in the nuclei of epithelial cells in the IS, caput and corpus of the epididymis. In the cauda, an intense reaction to AQP9 was observed in the epithelial border. In the IS, caput and corpus, the reactivity for AQP9 differed from those observed in domestic animals. The cauda showed a pattern similar to that previously described. These results indicate that AQPs 1 and 9 have reversed locations and roles in rams, suggesting activity variations related with fluid and solute absorption throughout the epididymis.

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Section: Discussionmentioning

confidence: 99%

Immunolocalization of Aquaporins 1 and 9 in the Ram Efferent Ducts and Epididymis

Schimming

Pinheiro

Matteis

et al. 2015

Reprod Domestic Animals

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“…Clear cells or light cells in the epididymides have been described in rat (Hamilton 1975), hamster (Nicander and Glover 1973), and African giant rat (Oke et al 1988) and are also seen in M. radiata. However, the absence of clear cells has been reported for rhesus monkey (Ramos and Dym 1977), guinea pig (Hoffer and Greenberg 1978), bull (Goyal 1985), goat (Goyal and Williams 1991), and dog (Schimming et al 1997). Clear cells are regarded as an important component of the mammalian epididymis, because of its presence in the rat epididymis, which is regarded as the prototype of epididymal structure in general.…”

Section: Male Reproductive System In Bonnet Monkey 57mentioning

confidence: 96%

Anatomical aspects of the male reproductive system in the bonnet monkey (Macaca radiata)

2009

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The normal anatomy of the male reproductive system in Macaca radiata is presented here. The external genitalia consist of a triangular button-shaped glans penis. The corpus cavernosum, and spongiosum form the vascular component of the penis and the baculum or os penis forms the non-vascular erectile component. The baculum is one of the longest in the genus macaques. The scrotal sac is non-pigmented, slightly pendulous, with scattered hairs, faintly corrugated, and does not reach the ischial callosities in the sitting posture. The testicles are ovoid in shape without appendix. Right and left testicular arteries originate at the level of the inter-vertebral disc between T12-L1 and L2-L3, respectively. Seminiferous tubules present mixed stages of spermatogenesis, i.e. single/multistage. The epididymis is crescent shaped, attached to the postero-lateral border of the testis without an appendix. Light microscopic observation revealed a characteristic high columnar epithelium with stereocilia. Clear cells or light cells are seen in the caudal region. The ductus deferens display a lumen lined by pseudo-stratified columnar epithelium separated by concentric layers of smooth muscle cells covered by serosa. The seminal vesicles are pyramidal in shape, prominently projecting above the urinary bladder, and are the largest of the accessory glands, typical of polyandrous primate genera. The prostate is conical in shape. Its base is in contact with the trigone of the bladder. Its posterior surface shows a transverse cleft separating an upper quarter, the cranial lobe, from the lower three-quarters of the gland. Compared with other macaques there are many distinguishing features in M. radiata. Excellent adaptability and spermatogenic efficiency in the laboratory environment makes this animal a good primate model for andrological research.

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“…Fragments of testis; initial segment, caput, corpus and cauda of the epididymis, and vas deferens were collected. Previous histological epididymal zonation of the dog had been accomplished according to Schimming et al (1997). The biological samples were immediately immersed in fixative, and some alternate samples were randomly assigned and snap frozen.…”

Section: Animal and Tissuesmentioning

confidence: 99%

Immunolocalization of aquaporins 1, 2 and 7 in rete testis, efferent ducts, epididymis and vas deferens of adult dog

et al. 2008

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The transepithelial movement of water into the male reproductive tract is an essential process for normal male fertility. Protein water channels, referred to as aquaporins (AQPs), are involved in increasing the osmotic permeability of membranes. This study has examined the expression of AQP1, AQP2, and AQP7 in epithelial cells in adult dog efferent ducts, epididymis, and vas deferens. Samples of dog male reproductive tract comprising fragments of the testis, initial segment, caput, corpus and cauda epididymidis, and vas deferens were investigated by immunohistochemistry and Western blotting procedures to show the localization and distribution of the AQPs. AQP1 was noted in rete testis, in efferent ducts, and in vessels in the intertubular space, suggesting that AQP1 participated in the absorption of the large amount of testicular fluid occurring characteristically in the efferent ducts. AQP2 expression was found in the rete testis, efferent ducts and epididymis, whereas AQP7 was expressed in the epithelium of the proximal regions of the epididymis and in the vas deferens. This is the first time that AQP2 and AQP7 have been observed in these regions of mammalian excurrent ducts, but their functional role in the dog male reproductive tract remains unknown. Investigations of AQP biology could be relevant for clinical studies of the male reproductive tract and to technologies for assisted procreation.

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HISTOLOGY OF THE DUCTUS EPIDIDYMIDIS IN THE DOG (Canis familiaris, L.)

Cited by 9 publications

References 0 publications

Immunolocalization of Aquaporins 1 and 9 in the Ram Efferent Ducts and Epididymis

Immunolocalization of Aquaporins 1 and 9 in the Ram Efferent Ducts and Epididymis

Anatomical aspects of the male reproductive system in the bonnet monkey (Macaca radiata)

Immunolocalization of aquaporins 1, 2 and 7 in rete testis, efferent ducts, epididymis and vas deferens of adult dog

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