Histology of Damage Caused by Euschistus heros (F.) Nymphs in Soybean Pods and Seeds

Tessmer, Magda Andréia; Kuhn, Taciana Melissa de Azevedo; Appezzato‐da‐Glória, Beatriz; Lopes, João Roberto Spotti; Erler, Greice; Bonani, Jean Patrick

doi:10.1007/s13744-021-00931-w

Cited by 4 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nymphs, being generally more sensitive than adults, may be influenced to a greater extent by interactions with other species. 24 Although there was no statistically significant difference when evaluating the competitor E. heros, a reduction in longevity was observed in intraspecific interactions. The longevity of D. melacanthus was negatively affected by intraspecific interactions and the presence of E. heros.…”

Section: Discussionmentioning

confidence: 88%

See 1 more Smart Citation

Intraspecific and interspecific interaction and fitness cost of stink bugs Euschistus heros, Diceraeus melacanthus, and Piezodorus guildinii in soybean

Santos,

Baldin,

Lima

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

BackgroundThe most common pentatomid species in soybean crops are Euschistus heros (F.), Piezodorus guildinii (W.), and Diceraeus melacanthus (D.), causing a significant reduction in yield. It is known that these stink bugs inhabit the reproductive structures of soybeans simultaneously; however, there are few studies addressing their intraguild interactions, as well as aspects of possible competition between them in plants. Thus, the inter‐ and intraspecific interactions of these stink bugs were evaluated in laboratory and field conditions, throughout the duration of the instars and adulthood, including longevity, mortality, and the number of eggs per female.ResultsE. heros had a higher competitive capacity in the interaction with D. melacanthus and P. guildinii, negatively interfering in the abundance or development (duration of instar, fertility, and mortality) of these stink bugs in soybean crops. This interference may act on the natural balance of these insect pests. Mortality of adults in interactions containing E. heros as a competitor or not showed that this species was not affected by the other species under field conditions. In the scenario where D. melacanthus was evaluated, it was observed that the presence of other species caused higher mortality in D. melacanthus. Additionally, higher P. guildiniii mortality was observed in interspecific interactions.ConclusionOur results suggest that E. heros has a greater competitive ability in the soybean crop, followed by D. melacanthus and P. guildinii. Therefore, the results found justify the greater abundance of E. heros and help to explain the increasing occurrence of D. melacanthus in soybean crops, contributing to new directions for understanding the interaction of the soybean stink bug complex.This article is protected by copyright. All rights reserved.

show abstract

Section: Discussionmentioning

confidence: 88%

“…melacanthus prolonged the developmental period of the fourth instar of that species. Nymphs, being generally more sensitive than adults, may be influenced to a greater extent by interactions with other species 24 …”

Section: Discussionmentioning

confidence: 99%

Intraspecific and interspecific interaction and fitness cost of stink bugs Euschistus heros, Diceraeus melacanthus, and Piezodorus guildinii in soybean

Santos,

Baldin,

Lima

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…To our knowledge, characterization of the feeding behavior and comparison among instars has not been studied in E. heros . A study used histological examination to evaluate the sites used by nymphs (second–fifth instars) on soybean pods based on the position of the salivary sheath (Tessmer et al 2022). The findings of the study revealed that nymphs of all stages reached the vascular bundles (xylem) and seed coat and seed endosperm.…”

Section: Discussionmentioning

confidence: 99%

Electropenetrography study of Euschistus heros (F.) (Heteroptera: Pentatomidae) nymphs feeding at different sites of the soybean pod

Lucini

Panizzi

Semeão³

2023

Environmental Entomology

View full text Add to dashboard Cite

This study aimed to characterize and correlate the stylet penetration behaviors of nymphs of the Neotropical brown stink bug, Euschistus heros (F.), on immature soybean pods. Waveforms were obtained using electropenetrography (EPG). The findings revealed that the nymphs exploited the xylem vessels and the seed tegument or endosperm. Primarily 4 phases were characterized: nonfeeding, pathway, salivation, and ingestion. The waveforms of each phase were similar in appearance across instars. The biological meanings of waveforms were based on visual observations, comparison with waveforms of adults, and histological studies. Np represents the insect resting or walking on soybean pod surface. Eh1 represents the first contact between the mouthparts (stylets) and plant tissue. Eh2 represents xylem sap ingestion, and Eh3 represents seed activities (including tegument and endosperm). The number of waveform events did not differ among instars for all waveforms. However, for Eh3, fifth instars performed more activities than other instars. The second instars had the smallest value, and third and fourth instars had intermediate values. For total duration, all waveforms differed among instars. Np duration was shorter for third compared with second and fourth instars and intermediate for fifth instar. For Eh1, second and third instars had the longest duration (1.5× to 2× greater) compared with fourth and fifth instars. For Eh2 and Eh3, the second-instar showed the longest (~2× greater) and shortest durations, respectively. Overall, this study provides important insights into the feeding behavior of E. heros nymphs so that effective pest management programs can be developed to contain this pest.

show abstract

“…Some protocols for plant tissue, including seeds, that used glycol methacrylate and hydroxyethyl-methacrylate as embedding resin that is a hydrophilic resin that avoids the use of organic solvents for processing (Tessmer et al, 2022;Zraidi et al, 2003). The inconvenience with methacrylate resins is that are between 5 to 12.5 times more expensive than paraffin, they need longer period for infiltration, embedding and a difficult ultra violet-dependent polymerization (Santos de Oliveira, 2015;Samavedi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Fast seed histology protocols: Benzene derivatives-free vs xylene-dependent

Benavides-Acevedo

Torres-Segura

2022

Agron. Mesoam.

View full text Add to dashboard Cite

Introduction. Seeds are complex structures that allow the biological and crop propagation of plants. Seed histology can be used for teaching, researching, and for pathological diagnostic. Histology protocols are commonly divided into 5 different stages: fixation, processing, cutting, staining, and mounting. Xylene is a dangerous reagent used during the processing, staining, and mounting of histological specimens that can contaminate the environment and is toxic for users. Objective. To compare two new protocols for seed histology accelerated with microwave, tested on seeds of economic importance species. Materials and methods. The experiments were done between January and May of 2022 at the Centro de Investigaciones en Granos y Semillas (CIGRAS) of the Universidad de Costa Rica. The compared protocols were: a benzene derivatives-free (BDF) and a xylene-dependent (XD). Seeds of Carica papaya L. (Caricaceae) var. Pococí, Coffea arabica L. (Rubiaceae) var. Obata, Glycine max L. (Fabaceae) var. CIGRAS-06, Phaseolus vulgaris L. (Fabaceae) var. Tayni, Oryza sativa L. (Poaceae) var. Lazarroz FL, and Zea mays L. (Poaceae) var. EJN-2 were used. Three technical replicates of five seeds of each species were tested with the XD and BDF protocols, on different days each replicate. Results. The photomicrographs obtained with both protocols showed that the samples maintained the morphology integrity of embryo, endosperm or cotyledons, and other seed structures. BDF and XD protocols produced seed histology slides and microphotographs. PAS-Coomassie Blue staining made a good differentiation of carbohydrates and proteins. Fastness of both protocols is a benefit compared with other protocols for plant histology that can take several days or even weeks. Conclusions. The BDF and XD protocols were suitable for seed histology analysis of bean, coffee, maize, papaya, and soybean, slides were obtained in less than 5 hours. BDF protocol is the first for plant tissue processing that does not use benzene derivatives and that uses paraffin as embedding medium.

show abstract

Histology of Damage Caused by Euschistus heros (F.) Nymphs in Soybean Pods and Seeds

Cited by 4 publications

References 22 publications

Intraspecific and interspecific interaction and fitness cost of stink bugs Euschistus heros, Diceraeus melacanthus, and Piezodorus guildinii in soybean

Intraspecific and interspecific interaction and fitness cost of stink bugs Euschistus heros, Diceraeus melacanthus, and Piezodorus guildinii in soybean

Electropenetrography study of Euschistus heros (F.) (Heteroptera: Pentatomidae) nymphs feeding at different sites of the soybean pod

Fast seed histology protocols: Benzene derivatives-free vs xylene-dependent

Contact Info

Product

Resources

About