Histologically resolved small RNA maps in primary focal segmental glomerulosclerosis indicate progressive changes within glomerular and tubulointerstitial regions

Williams, Anna; Jensen, David M; Pan, Xiaoqing; Liu, Pengyuan; Liu, Jing; Huls, Sean; Regner, Kevin R.; Iczkowski, Kenneth A.; Wang, Feng; Li, Junhui; Gallan, Alexander J.; Wang, Tao; Baker, Maria Angeles; Liu, Yong; Lalehzari, Nava; Liang, Mingyu

doi:10.1016/j.kint.2021.12.030

Cited by 17 publications

(10 citation statements)

References 36 publications

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“…Findings from this work concerning downregulation of leucine tRFs and Met tRFs and upregulation of initiator Met tRFs in undifferentiated and APOL1 HR HUPECs are compatible with prior reports that leucine tRFs regulate translational activity through regulation of ribosomal biogenesis 26 , 27 and that Met tRFs inhibit translational initiation. 28 Small RNA-seq has been conducted recently on microdissected healthy kidneys and FSGS kidneys, including glomerular samples, 44 but cellular resolution when quantifying small RNA levels in kidney tissue has not been achieved.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Human Podocytes In Vitro: Effects of Differentiation and APOL1 Genotype

Yoshida

Latt²,

Rosenberg³

et al. 2023

Kidney International Reports

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Human Podocytes In Vitro: Effects of Differentiation and APOL1 Genotype

Yoshida

Latt²,

Rosenberg³

et al. 2023

Kidney International Reports

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“…Previous studies have reported that hsa-mir-1231 [571], STAT3 [572], STAT1 [573], GATA2 [574] and GATA3 [575] are related to cardiovascular diseases. Hsa-mir-21-5p [576] and PAX2 [577] are believed to be associated with FSGS. Liu et al [578], Park et al [579], Yang et al [580], Yu et al [581] and Forero-Delgadillo et al [582] mentioned that hsa-mir-21-5p, STAT3, STAT1, GATA2 and PAX2 were related to other kidney diseases.…”

Section: Discussionmentioning

confidence: 99%

Identification of biomarkers and pathways for focal segmental glomerulosclerosis using next generation sequencing data and bioinformatics analysis

Vastrad¹,

Vastrad²

2022

Preprint

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Focal segmental glomerulosclerosis (FSGS) is a renal disease leading threat to human health around the world. Here we aimed to explore novel molecular and potential therapeutic targets in FSGS through adopting integrated bioinformatics tools. Next generation sequencing (NGS) data of GSE197307 was available from Gene Expression Omnibus (GEO) database. Furthermore, differentially expressed genes (DEGs) were screened using the DESeq2 package in R software. Gene ontology (GO) and REACTOME pathway enrichment analyses of DEGs were performed via g:Profiler. Then, the protein-protein interaction (PPI) network, miRNA- hub gene regulatory network and TF-hub gene regulatory network were constructed using the HIPPIE, miRNet and NetworkAnalyst databases. Hub genes were validated using the receiver operating characteristic curve (ROC) analysis. By performing DEGs analysis, 488 up regulated genes and 488 down regulated genes were successfully identified from GSE197307, respectively. And they were mainly enriched in the terms of multicellular organismal process, cell periphery, protein binding, metabolism, immune system process, signaling receptor binding and immune system. Based on the data of protein-protein interaction (PPI), miRNA-hub gene regulatory network and TF-hub gene regulatory network, the top hub genes were ranked, including ILK, DDX5, MATR3, ALB, FOS, MKI67, PLK1, TNF, LCK and GTSE1. Bioinformatics analysis of NGS data identified signaling pathways and hub genes, potentially representing molecular mechanisms for the occurrence, progression, and risk prediction in FSGS.

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“…Wu et al also demonstrated that miR-30s were consistently downregulated in podocytes of FSGS patients and PAN-treated rats, and identified that downregulation of miR-30s results in calcium/calcineurin signaling activation, thus leading to podocyte injury in FSGS ( Wu et al, 2015 ). In addition, other studies have also reported the involvement of some other dysregulated miRNAs in FSGS, such as miR-135a ( Yang et al, 2017 ), miR-155 ( Ramezani et al, 2015 ), miR-206 ( Guo et al, 2016 ), miR-150 ( Qi et al, 2020 ), miR-106a ( Xiao et al, 2018 ) and miR-146-5p ( Williams et al, 2022 ).…”

Section: Podocytopathiesmentioning

confidence: 94%

Pathogenic Role of MicroRNA Dysregulation in Podocytopathies

Liu

Chen

et al. 2022

Front. Physiol.

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MicroRNAs (miRNAs) participate in the regulation of various important biological processes by regulating the expression of various genes at the post-transcriptional level. Podocytopathies are a series of renal diseases in which direct or indirect damage of podocytes results in proteinuria or nephrotic syndrome. Despite decades of research, the exact pathogenesis of podocytopathies remains incompletely understood and effective therapies are still lacking. An increasing body of evidence has revealed a critical role of miRNAs dysregulation in the onset and progression of podocytopathies. Moreover, several lines of research aimed at improving common podocytopathies diagnostic tools and avoiding invasive kidney biopsies have also identified circulating and urine miRNAs as possible diagnostic and prognostic biomarkers for podocytopathies. The present review mainly aims to provide an updated overview of the recent achievements in research on the potential applicability of miRNAs involved in renal disorders related to podocyte dysfunction by laying particular emphasis on focal segmental glomerulosclerosis (FSGS), minimal change disease (MCD), membranous nephropathy (MN), diabetic kidney disease (DKD) and IgA nephropathy (IgAN). Further investigation into these dysregulated miRNAs will not only generate novel insights into the mechanisms of podocytopathies, but also might yield novel strategies for the diagnosis and therapy of this disease.

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Histologically resolved small RNA maps in primary focal segmental glomerulosclerosis indicate progressive changes within glomerular and tubulointerstitial regions

Cited by 17 publications

References 36 publications

Transcriptomic Analysis of Human Podocytes In Vitro: Effects of Differentiation and APOL1 Genotype

Transcriptomic Analysis of Human Podocytes In Vitro: Effects of Differentiation and APOL1 Genotype

Identification of biomarkers and pathways for focal segmental glomerulosclerosis using next generation sequencing data and bioinformatics analysis

Pathogenic Role of MicroRNA Dysregulation in Podocytopathies

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