Histological Techniques for Decalcified Bone and Cartilage

Kang, Qian; LaBreck, Jackie C.; Gruber, Helen E.; An, Yuehuei H.

doi:10.1007/978-1-59259-417-7_13

Cited by 12 publications

(14 citation statements)

References 12 publications

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“…Mice were injected intra-articularly with PBS or HGP-CK2.1 (6 μM) or HGP 6 weeks post-DMM surgery ( n = 6/group) and were compared to sham-operated mice. Sample sections were stained using Safranin O ( red ) and fast green ( turquoise ), and scoring was performed in four compartments of the knee using a modified semiquantitative scoring scale [ 19 ]. Mice injected with HGP-CK2.1 showed the greatest cartilage repair compared to the sham-operated group.…”

Section: Resultsmentioning

confidence: 99%

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CK2.1, a bone morphogenetic protein receptor type Ia mimetic peptide, repairs cartilage in mice with destabilized medial meniscus

Akkiraju

Srinivasan

et al. 2017

Stem Cell Res Ther

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BackgroundOsteoarthritis (OA) of the knee involves degeneration of articular cartilage of the diarthrodial joints. Current treatment options temporarily relieve the joint pain but do not restore the lost cartilage. We recently designed a novel bone morphogenetic protein receptor type I (BMPRI) mimetic peptide, CK2.1, that activates BMPRIa signaling in the absence of bone morphogenetic protein (BMP). Our previous research demonstrated that CK2.1 induced chondrogenesis in vitro and in vivo; however, it is unknown if CK2.1 restores damaged articular cartilage in vivo. In this study, we demonstrate that CK2.1 induced articular cartilage (AC) repair in an OA mouse model.MethodsWe designed hyaluronic acid (HA)-based hydrogel particles (HGPs) that slowly release CK2.1. HGP-CK2.1 particles were tested for chondrogenic potency on pluripotent mesenchymal stem cells (C3H10T1/2 cells) and locally injected into the intra-articular capsule in mice with cartilage defects. C57BL/6J mice were operated on to destabilize the medial meniscus and these mice were kept for 6 weeks after surgery to sustain OA-like damage. Mice were then injected via the intra-articular capsule with HGP-CK2.1; 4 weeks after injection the mice were sacrificed and their femurs were analyzed for cartilage defects.ResultsImmunohistochemical analysis of the cartilage demonstrated complete repair of the AC compared to sham-operated mice. Immunofluorescence analysis revealed collagen type IX production along with collagen type II in the AC of mice injected with HGP-CK2.1. Mice injected with phosphate-buffered saline (PBS) and HGP alone had greater collagen type X and osteocalcin production, in sharp contrast to those injected with HGP-CK2.1, indicating increased chondrocyte hypertrophy.ConclusionsOur results demonstrate that the slow release HGP-CK2.1 drives cartilage repair without the induction of chondrocyte hypertrophy. The peptide CK2.1 could be a powerful tool in understanding the signaling pathways contributing to the repair process, and also may be used as a potential therapeutic for treating degenerative cartilage diseases such as OA.

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Section: Resultsmentioning

confidence: 99%

“…Femur samples ( n = 6/group) were paraffin embedded and sectioned at 6-μm thickness. Sectioned slide samples were stained using Safranin O and fast green staining [ 19 , 20 ]. Scoring was performed in four compartments of the knee using a modified semiquantitative scoring scale as described previously [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

CK2.1, a bone morphogenetic protein receptor type Ia mimetic peptide, repairs cartilage in mice with destabilized medial meniscus

Akkiraju

Srinivasan

et al. 2017

Stem Cell Res Ther

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“…The areas showing the damaged cartilage were cut into 2-mm thick slices, including a rim of the subchondral bone, and processed for histological examination. Sections were stained with Safranin O with Light Green counter-stain (Kang et al, 2003). Sections were examined and graded based on the most advanced state of OA in the section, irrespective of whether osteophytes were present (Pritzker et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

Analytical Study of Clinicopathological Data of Saudi Patients with Osteoarthritis Subjected to Total Knee Arthroplasty

et al. 2019

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Knee osteoarthritis (OA) is a common disabling disease. Epidemiological studies have revealed various risk factors for OA, including sex, aging, obesity, occupational illnesses, and chronic diseases. Here we evaluate the clinical, pathological, and radiological findings of knee OA in a subset of Saudi patients who were subjected to total knee replacement (TKA). The study population included 30 Saudi patients with knee OA who were operated by TKA (from

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“…Tissues were sampled and collected in 10% buffered formalin. For histologic examination, the vertebral columns of all affected toads were decalcified (Kang et al., 2003). Transverse sections at intervertebral joints sections of the vertebral column were taken, embedded in paraffin, sectioned at 4 μm and stained with hematoxylin and eosin (HE) techniques.…”

Section: Methodsmentioning

confidence: 99%

Radiographic and histological evidence of metabolic bone disease in gliding leaf frogs (Agalychnis spurrelli)

Galante-Mulki

Alvear-Santos

Santamaría-Naranjo

et al. 2019

Heliyon

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Bone alterations due to metabolic bone disease in captive animal populations can have a negative impact on repopulation and research initiatives. This investigation has the purpose of describing the principal radiographic and anatomopathological findings present in nine gliding leaf frogs ( Agalychnis spurrelli ) kept in captivity with alterations in their spines and long bones. The observed histopathological findings were in the canalis vertebralis, paraspinal muscle and long bones, and included deformed bones with alteration of the adjacent tissues, alterations in the ossification process, bone degeneration and resorption, decreased number of osteocytes and deposition of osteoid and fibrous material in the compact bone tissue. Additionally, the spinal cord showed compressed white matter, chronic meningitis in the duramater, alteration in the number of glial cells and loss of delimitation between the gray and white matter. Radiographical changes were found mainly in the long bones and included moth-eaten osteolysis, solid periosteal reaction, bone deformities, cortical tunneling and inflammation of adjacent soft tissues. Also, pathological fractures of the femur and urostyle were observed together with spinal column deviations with increased bone density.

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Histological Techniques for Decalcified Bone and Cartilage

Cited by 12 publications

References 12 publications

CK2.1, a bone morphogenetic protein receptor type Ia mimetic peptide, repairs cartilage in mice with destabilized medial meniscus

CK2.1, a bone morphogenetic protein receptor type Ia mimetic peptide, repairs cartilage in mice with destabilized medial meniscus

Analytical Study of Clinicopathological Data of Saudi Patients with Osteoarthritis Subjected to Total Knee Arthroplasty

Radiographic and histological evidence of metabolic bone disease in gliding leaf frogs (Agalychnis spurrelli)

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