Histological Study on the Male Reproductive Organs of Mouse CF1 Treated With Boron

Espinoza-Navarro, Omar; Vilaxa, Arnaldo; Granifo, L; Rojas, Sara; Rodríguez, Héctor

doi:10.4067/s0717-95022007000200017

Cited by 8 publications

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“…This would represent the formation of true testicular pacemarkers for the regulation of the motility of the seminiferous tubules and presumably in the fertility of the adult man. All these immunohistochemical aspects here analysed must be considered in future studies of male reproductive organs in human and others models species (Espinoza-Navarro & Bustos-Obregón, 2005;Holt et al, 2004;Espinoza-Navarro et al, 2007). RESUMEN: El objetivo de este trabajo fue identificar la presencia de células interticiales de Cajal, células musculares lisas, células nerviosas y células que expresan receptores de andrógeno en el testículo de humano adulto, usando inmunohistoquímica específica para: c-kit/CD-117, músculo liso actina específico (ASMS), neurofilamentos (N) y para receptores de andrógenos (AR).…”

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Histological and Functional Organization in Human Testicle: Expression of Receptors c-kit and Androgens

et al. 2008

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The objective of this work was identify the presence of interstitial cells of Cajal, muscle cells, nerves and androgen receptor positive cells in adult human testicle, using immunohistochemical detection for c-kit/CD-117, actin smooth muscle specific (ASMS), neurofilament (N) and androgen receptor (AR), respectively. The samples were obtained from patients (n= 10) with diagnosis of prostate cancer, with surgery of orchiectomy. Subsequently were processed by histology and for immunohistochemistry using specific antibodies. It showed the presence of cells c-kit/CD-117, with diverse degrees of positivity, distributed mainly in the interstitial peritubular area of the human testicle. The peritubular myoides cells were positive to the presence of the actin smooth muscle and androgen receptor. The neurofilaments elements (+) only were observed in the vascular tunic. The specific immunohistochemistry describe the presence of the interstitial cells of Cajal in human testicular interstitium, opening a new perspective for the functional interpretation of the testicular cellularity and tubular motility. Possibly associated functionally to peribubulars cells of smooth muscle to regulate the mobility of the seminiferous tubules, whose integration and function would be androgen dependent. The cells that express the c-kit receptor, were found exclusively in the interstitial compartment. This cellular type in addition of the muscular cells of peritubules and the absence of nervous fibers to the interior of the testicle, could be responsible for the regulation of tubular mobility, as it happens in the gastrointestinal apparatus.

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Histological and Functional Organization in Human Testicle: Expression of Receptors c-kit and Androgens

et al. 2008

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“…These biological markers play an important role in the detection of the impact of the exposition and the prediction of risks in other populations. The problem of boron is normal in the few places where there are boron deposits, such as in Turkey, France, California and Chile, nevertheless the exposed population has the right to know the real effects of high concentrations of boron in public health (Luster et al, 1998;Spurgeon, 2004;Espinoza-Navarro). The present research study spefically will use as a biological model to Drosophila melanogaster to determine the effect of high concentrations of boron in their reproductive patterns.…”

Section: Introductionmentioning

confidence: 96%

Alteration of the Reproductive Patterns in Drosophila melanogaster by Effects of High Concentrations of Boron on In Vitro Cultured Medium

Espinoza-Navarro

Rodríguez

et al. 2009

Int. J. Morphol.

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“…With researches based on dosage and time, the effects of boron mineral on the reproductive system have mostly been tried to put forth on rats and mice (8,15,16). Ince et al, has observed that diet with high boron concentration decreases lipid peroxidation and improves antioxidant mechanism in a study done with rats (11).…”

Section: Introductionmentioning

confidence: 99%

Effects of boron (sodium pentaborate), added instead of Tris components, on freezing and post-thaw quality of Angora buck semen

TEKİN¹

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:In this research, we aimed to evaluate freezing and in vitro results of post-thawed Angora goat semen parameters using boron (sodium pentaborate, Na 2 O.5B 2 O 3 .10H 2 O) instead of other components and as an additive in Tris extender. Mature Angora bucks, with proven fertility, accustomed to artificial vagina, and with normospermic values have been used in the study. Research was carried out with 9 different extenders: Boron extenders prepared by adding different amounts of Boron instead of glucose into standard Tris extender (GIB, 1-a and 1-b, 1-c, 1-d), the standard Tris extenders prepared by only addition of different amounts of Boron (BAD; 2-a, 2-b, 2-c, 2-d) and standard Tris extender (Control). Post-thaw spermatological examinations have been done with sperm analyzer (CASA) and results were recorded as follows; the highest total and progresive motility scores between 9 different extenders belonged to 1-c with 63,34 ± 2,32 and 1-d with %38,27± 0,74, highest VCL, VSL, VAP, LIN, STR and WOB average scores were found in 2-d extender (p<0,001). The percentage of lowest dead spermatozoa rate % 42,175± 1,59 was observed with GIB between main groups (p<0,01). Between extenders, lowest dead spermatozoa rates were recorded as % 36,04± 2,58 and % 36,17± 3,66 in 2-b and 1-c (p<0,001). When abnormal spermatozoa rates were examined, lowest abnormal spermatozoon rate belonged to 2-c extender with 21,29± 0,97 (p<0,001) and abnormal acrosome rates were obtained from %6,57± 1,29 1-a extender with (p<0,01). In conclusion, some positive effects of sodium pentaborate on post-thaw spermatological parameters of Angora buck semen have been demonstrated.Key words: Angora bucks, boron, semen, spermatological parameters.Ankara tekesi spermasının dondurulmasında Tris sulandırıcı bileşenleri yerine katilan borun (sodyum pentaborat) çözüm sonu sperma kalitesi üzerine etkisi Özet: Bu araştırmada, Ankara tekesi spermasının dondurulması ve çözdürülmesinde, borun (sodyum pentaborat, Na 2 O.5B 2 O 3 .10H 2 O) Tris sulandırıcısına eklenerek ve bileşenleri yerine katılarak hazırlanmış solüsyonlarla, elde edilecek in vitro sonuçların değerlendirilmesi amaçlandı. Çalışma materyali olarak, dölverimi kanıtlanmış ve suni vaginaya sperma vermeye alıştırılmış ergin Ankara tekelerinin normospermik ejakülatları alındı. Çalışmada standart Tris sulandırıcısından glikoz çıkarılıp farklı miktarlarda bor eklenerek hazırlanmış sulandırıcılar (GYB; 1-a, 1-b, 1-c, 1-d), standart Tris sulandırıcısına farklı miktarlarda bor eklenerek hazırlanmış sulandırıcılar (BEK;2-a, 2-b, 2-c, 2-d) ve standart Tris sulandırıcısı (Kontrol) olmak üzere 9 farklı sulandırıcı kullanıldı. Sperma analiz sistemi (CASA) ile total motilite ve progresif motilite değerlerine bakıldığında sırasıyla en yüksek değerinlerin 63,34 ± 2,32 ile 1-c ve %38,27± 0,74 ile 1-d sulandırıcılarına VCL, VSL, VAP, LIN, STR, WOB verilerinin en yüksek 2-d sulandırıcısına ait olduğu belirlendi (p<0,001). Ölü spermatozoon oranı değerlendirildiğinde en düşük ölü spermatozoon oranının % 36,04± 2,58 v...

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Histological Study on the Male Reproductive Organs of Mouse CF1 Treated With Boron

Cited by 8 publications

References 0 publications

Histological and Functional Organization in Human Testicle: Expression of Receptors c-kit and Androgens

Histological and Functional Organization in Human Testicle: Expression of Receptors c-kit and Androgens

Alteration of the Reproductive Patterns in Drosophila melanogaster by Effects of High Concentrations of Boron on In Vitro Cultured Medium

Effects of boron (sodium pentaborate), added instead of Tris components, on freezing and post-thaw quality of Angora buck semen

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