Histological study on the effect of vitamin C on ischemia–reperfusion injury in the adult rat ovary

Abstract

BackgroundOvarian torsion may cause serious complications such as infertility in young women. Conservative management includes detorsion and reperfusion of the twisted segment. However, it may have local and systemic consequences due to production of large amounts of reactive oxygen species during reperfusion of ovaries. Aim of workThe present work aimed to study the possible histological and immunohistochemical changes due to ischemia-reperfusion injury in rat ovaries and the possible protective effect of vitamin C as an antioxidant. Materials and methodsA total of 32 albino rats were divided into four groups. Group I was the control sham-operated group (either sham operated only, or with vitamin C administration). In group II rats, ovarian ischemia was induced by torsion of the right adnexa. In rats of group III, 4 h of ischemia followed by reperfusion was performed. In rats of group IV, 4 h of ischemia was followed by 50 mg/kg vitamin C administration, which was injected intravenously, and then reperfusion was performed. Except for the ischemia group, all other groups were subdivided into two subgroups from which the right ovaries were surgically removed either after 5 h or after 2 weeks of starting the experiment. From the ischemia group ovarian samples were taken after 5 h only. Specimens were processed for paraffin sections and stained with H&E and with an immunohistochemical stain for apoptotic marker p53. Image analysis and statistical analysis of the obtained results were carried out. ResultsSevere vascular congestion, edema, hemorrhage, and increased P53 immunoreaction were detected in the ovaries after ischemia, which became less marked after reperfusion and considerably improved with vitamin C administration, especially after 2 weeks. Conclusion Vitamin C treatment can help in protecting the ovaries from ischemia-reperfusion injury after detorsion.

Determination of the Protective Impact of Hypericum perforatum Extract Against Ovarian Ischemia and Reperfusion in Rats

“…From these results, it was concluded that the reperfusion after ischemia had done serious damage to the tissue and its cells. A previous study determined that p53 immunoreactivity in control group ovaries tissue was hardly observed but it was observed in the ischemia group (25). We found that p53 immunoreactivity did not show a statistical difference between groups.…”

The impact of electromagnetic radiation (2.45 GHz, Wi-Fi) on the female reproductive system: The role of vitamin C

“…It protects body tissues against oxidative damage. Nasr et al (2014) showed the ameliorating effect of vit C after ischemia-reperfusion in adult rat ovary. Abbasi et al (2016) investigated the effect of vit C on monosodium glutamate inducing changes in the rats ovary.…”

The Effect of Vitamin C on Ischemia-Reperfusion Injury: A Systematic Review

“…Vitamin C is a safe and easy drug that can protect the ovaries from ischemia-reperfusion damage caused by detour, which is the main conservative method for managing ovarian torsion (61). Sagsoz et al, who studied the effects of antioxidants on the ovaries, reported that vitamin C reduced ischemia-reperfusion of the ovaries in its early stages, which is better than verapamil, a calcium channel blocker (75).…”