Histological study on ouabain immunoreactivities in the mammalian hypothalamus

Yamada, Hisao; Naruse, Mitsuhide; Naruse, Kiyoko; Demura, Hiroshi; Takahashi, Hakuo; Yoshimura, Manabu; Ochi, Junzo

doi:10.1016/0304-3940(92)90881-7

Cited by 53 publications

(23 citation statements)

References 13 publications

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“…However, no evidence has been available to support the presence of ouabain in the anterior pituitary. 13 In contrast, we have recently shown the presence of immunoreactive ouabain in the hypothalamus, 14 suggesting a hypothalamic origin of endogenous ouabain. 15 It is not known, however, whether the hypothalamic ouabain is released into the circulation, and if so, how the pituitary tumor affects the hypothalamic ouabain.…”

Section: Discussionmentioning

confidence: 86%

Does plasma immunoreactive ouabain originate from the adrenal gland?

Naruse

Tanabe

et al. 1994

Hypertension

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It was reported recently that the endogenous digitalis-like factor ouabain may mainly originate from the adrenal gland. To ascertain the pathophysiological significance of endogenous ouabain and to examine if it originates in the adrenal gland, we determined plasma immunoreactive ouabain levels in patients with various cardiovascular and endocrine diseases. Plasma immunoreactive ouabain levels were also determined in the adrenal venous blood by adrenal venous sampling. Plasma immunoreactive ouabain levels were significantly increased in patients with essential hypertension, primary aldosteronism, Cushing's syndrome, pheochromocytoma, acromegaly, and chronic renal failure. Plasma immunoreactive ouabain levels were decreased in patients with primary aldosteronism after unilateral adrenalectomy, acromegaly after pituitary adenomectomy, and chronic renal failure after hemodialysis. Plasma immunoreactive ouabain levels in patients after bilateral adrenalectomy were similar to those in healthy subjects. There was no significant step-up of immunoreactive ouabain levels in the adrenal vein from the peripheral vein in three patients, whereas one patient with hypertension and right adrenal tumor but without any known adrenal hormone excess showed higher plasma immunoreactive ouabain levels in the right adrenal vein than those in the peripheral vein. These results suggest an important pathophysiological significance of endogenous ouabain in various cardiovascular and endocrine diseases. It is unlikely that the adrenal gland is a major source of plasma ouabain, although a possible excess production of ouabain by the adrenal tumor remains to be elucidated.

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Section: Discussionmentioning

confidence: 86%

Does plasma immunoreactive ouabain originate from the adrenal gland?

Naruse

Tanabe

et al. 1994

Hypertension

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“…Furthermore, OLI and DLI are distributed in the neurons of the paraventricular and supraoptic nuclei, and in the fibers containing the substance distributed to the circumventricular area of the third ventricle. This has been demonstrated by immunohistochemistry using mono-and poly-clonal antiouabain antibodies and polyclonal anti-digoxin antibody (1,(11)(12)(13). The OLI is also found in the adrenal medulla (14).…”

Section: Discussionmentioning

confidence: 97%

Increases in Plasma Ouabainlike Immunoreactivity during Surgical Extirpation of Pheochromocytoma.

Komiyama¹,

Nishimura²,

Munakata³

et al. 1999

Hypertens Res

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The ouabainlike factor (OLF) is thought to be an important modulator of salt and water metabolism. Plasma OLF could be derived from the central nervous system and/or the adrenal gland. Since the adrenal medulla is of neural origin, the cytology of pheochromocytoma of adrenomedullary origin resembles that of neuronal cells. Ouabainlike immunoreactivity (OLI) is, in fact, present in the adrenal medulla as shown by immunohistochemistry.The plasma levels of catecholamines and OLI were significantly elevated during surgical extirpation of pheochromocytoma in this case. To clarify the origin of circulating OLI in a patient with pheochromocytoma, the relationship between plasma OLI and catecholamines during adrenalectomy was investigated. Plasma catecholamine levels exceeded the normal reference interval, and plasma OLI was positively correlated with the patient's plasma level of norepinephrine.The peak level during operation was about 10 times higher than the baseline level. Both levels reached a maximum when the tumor was mechanically pressed, and then gradually decreased thereafter.The level of OLI in the tumor was higher than that of the normal adrenal cortex. When OLI in the tumor was characterized by reversed-phase high-performance liquid chromatography, the retention time of OLI corresponded with that of authentic ouabain. These results suggest that the circulating OLI in this patients was derived mainly from the pheochromocytoma of adrenomedullary origin. (Hypertens Res 1999; 22: 135-139)

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“…This is partly due to structural similarities in the epitope of the antigen. In several studies using the immunohistochemical technique, with an anti-digoxin monoclonal antibody, the digitalis-like immunoreactive substances were localized in the hypothalamus or the adrenal medulla [18][19][20][21] . We previously described a histochemical technique to localize the immunoreactive products of anti-digoxin monoclonal antibodies in patients who were chronically treated with digoxin [17] .…”

Section: Identification Of Digitalis-like Immunoreactive Substancesmentioning

confidence: 99%

Increased digitalis-like immunoreactive substances in patients with hypertrophic cardiomyopathy

Hayashi

Ijiri²,

Toko³

et al. 2000

European Heart Journal

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Aims Although increased digitalis-like immunoreactive substances have been found in cases of hypertension and heart failure, no information is available about digitalis-like immunoreactive substances in patients with hypertrophic cardiomyopathy. We investigated digitalis-like immunoreactive substances in the plasma and biopsied specimens of patients with hypertrophic cardiomyopathy. Methods and ResultsIn 40 patients with hypertrophic cardiomyopathy (27 with the non-obstructive type and 13 with the obstructive type), the plasma concentration of digitalis-like immunoreactive substances was studied by fluorescence polarization immunoassay. Right ventricular endomyocardial biopsy specimens were analysed immunohistochemically, using a monoclonal antibody against digoxin. An increase in digitalis-like immunoreactive substances of more than 0·2 ng . ml 1 in plasma was found in six of 27 patients with non-obstructive hypertrophic cardiomyopathy (22·2%) and five of 13 with obstructive hypertrophic cardiomyopathy (38·4%). Under light microscopy, positive staining against the antibody was observed heterogeneously on some cardiocytes. In non-obstructive hypertrophic cardiomyopathy, digitalis-like immunoreactive substances in the plasma correlated with the left atrial dimension and inversely with the cardiac index. In obstructive hypertrophic cardiomyopathy, plasma and myocardial digitalis-like immunoreactive substances were positively correlated; they also correlated with left ventricular enddiastolic pressures. Under electron microscopy, digitalislike immunoreactive substances were detected at the sarcolemma in the free wall, T-tubules, intercalated discs and Z-bands of cardiocytes.Conclusions Increased digitalis-like immunoreactive substances in plasma and cardiocytes, which may have been caused by pressure and/or volume overload, were found in patients with hypertrophic cardiomyopathy. Digitalis-like immunoreactive substances may act on the sarcolemma of cardiocytes and be transported into the cytoplasm.

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Histological study on ouabain immunoreactivities in the mammalian hypothalamus

Cited by 53 publications

References 13 publications

Does plasma immunoreactive ouabain originate from the adrenal gland?

Does plasma immunoreactive ouabain originate from the adrenal gland?

Increases in Plasma Ouabainlike Immunoreactivity during Surgical Extirpation of Pheochromocytoma.

Increased digitalis-like immunoreactive substances in patients with hypertrophic cardiomyopathy

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