Histological study of micrografting in passionfruit

Ribeiro, Leonardo Monteiro; Nery, Lays Araújo; Vieira, Luciana Marques; Mercadante-Simões, Maria Olívia

doi:10.1007/s11240-015-0824-1

Cited by 29 publications

(17 citation statements)

References 25 publications

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“…According to Hull (), and Harries & Ding (), after entry into host cells, virus moves over short distance within plants through plasmodesmata (cell‐to‐cell trafficking) and for long distance through vascular tissue, usually the sieve elements of the phloem. Callus formation and establishment of vascular tissues between scions and rootstocks are necessary to ensure success and efficiency of micrografting (Estrada‐Luna et al ., ; Pina & Errea, ; Yin et al ., ; Melnyk et al ., ; Ribeiro et al ., ). The former makes it possible for virus to move over short distance by cell‐to‐cell trafficking, while the latter for long distance movement through vascular bundles between the scions and rootstocks (Hull, ; Harries & Ding, ).…”

Section: Discussionmentioning

confidence: 97%

“…Previous studies demonstrated that before establishment of vascular bundles in micrografts, the transmission of molecular and chemical signals between the scions and rootstocks is through cell‐to‐cell connections, that is callus formation (Pina & Errea, ; Yin et al ., ; Melnyk et al ., ). Sufficient transport of water and metabolites between the scions and rootstocks can only be ensured after establishment of vascular bundles (Richardson et al ., ; Pina & Errea, ; Melnyk et al ., ; Ribeiro et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Although information on histological studies of micrografting of grapevines has been quite limited, similar results were also found in other plant species such as Pistacia vera (Abousalim & Mantell, ), Olea europaea (Toroncoso et al ., ), Opuntia spp. (Estrada‐Luna et al ., ), Arabidopsis thaliana (Melnyk et al ., ) and Passiflora edulis (Ribeiro et al ., ). Certainly, time durations of histological process differed from plant species or experimental systems (Richardson et al ., , Fernández‐García et al ., ; Pina & Errea, , Flaishman et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Histological observation and virus localisation in micrografts are the two basic issues, which would help to better understand the mechanism involved in micrografting for virus diagnosing. There have been few histological studies in micrografts (Abousalim & Mantell, ; Richardson et al ., ; Estrada‐Luna et al ., ; Dumanoglu et al ., ; Ribeiro et al ., ), and virus localisation in micrografts has never been reported in the literature.…”

Section: Introductionmentioning

confidence: 95%

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Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Hao

Cui

et al. 2017

Annals of Applied Biology

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Development, histological process and Grapevine leafroll-associated virus-3 localisation were studied in micrografts of three scion/rootstock combinations: healthy/healthy, healthy/infected and infected/healthy. Earlier bud break and faster growth in scions of micrografts were obtained when the healthy shoot segments were used as scions, while earlier bud break in rootstocks and greater fresh weight of roots in micrografts were produced when the healthy shoot segments were used as rootstocks. All histological processes including callus initiation and formation in micrografting conjunctions, and initiation of new cambial cells followed by vascular bundle development connecting scions and rootstocks were similar in micrografts, regardless of the sanitary status of the scions and rootstocks used for micrografting. Virus infection in micrografting conjunctions and systematic infection in micrografts were much more efficient and faster in micrografting combination of the infected scions/healthy rootstocks than in the healthy scions/infected rootstocks. To the best of our knowledge, this is the first report addressing histological process of micrograft development and virus localisation in micrografts. In vitro culture system established in this study facilitates studies on the 'pure' impact of the viral infection on micrografting.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 95%

See 2 more Smart Citations

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Hao

Cui

et al. 2017

Annals of Applied Biology

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“…This propagation technique has been used for different purposes in several species, for example in Passifora edulis Sims to eliminate the mosaic virus that causes fruit hardening [5], in Anona muricata L. to determine compatibility with the rootstocks used [6] and in Citrus sinensis L. to find the best interaction in three CTV-tolerant rootstocks and optimize tree development [7]. It is also used in tree species such as Pinus patula Schiede ex Schltdl.…”

Section: Introductionmentioning

confidence: 99%

Anatomy of Rootstocks and Scions in Four Pine Species

Slc¹,

Av²,

Jl³

2017

Forest Res

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When performing grafts, it is thought that by using plants of the same species the engraftment rate will be high; when the above is not fulfilled, the grafter, type of graft or the origin of the scions is blamed, but the anatomy of the parts forming the graft is not considered. The aim of this study was to evaluate the anatomy of four pine species used as rootstock and scion to define which species to use as rootstock based on cambium shape and bark thickness. Before grafting, 2-cm stem fractions were cut from plants that were used as rootstocks and scions. The evaluated species were Pinus patula, P. greggii, P. leiophylla and P. teocote. Cuts of 10 μm in thickness were made to determine bark and xylem thickness. Photographs were also taken with a photomicroscope to observe the "shape" of the vascular cambium. The experimental design was completely randomized with a 4x2 factorial arrangement, using 12 measurements as the sample size with four replicates. P. patula had greater bark thickness, while P. leiophylla obtained the greatest xylem thickness. In the images of the anatomical cuts, it was determined that the vascular cambium is continuous for all the species both in rootstock and in scions, but it is only circular in the P. teocote and P. leiophylla rootstocks, and the P. leiophylla scions did not present this feature. Based on the anatomy of the cambium, P. greggii, P. patula and P. teocote scions have greater possibilities of engraftment if they are grafted onto P. teocote and P. leiophylla because the cambium is circular. Tissue anatomy is one of the areas that must be known before making grafts in order to make appropriate decisions regarding the materials to be used.

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Plant grafting: insights into tissue regeneration

2016

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For millennia, people have cut and joined different plants together through a process known as grafting. The severed tissues adhere, the cells divide and the vasculature differentiates through a remarkable process of regeneration between two genetically distinct organisms as they become one. Grafting is becoming increasingly important in horticulture where it provides an efficient means for asexual propagation. Grafting also combines desirable roots and shoots to generate chimeras that are more vigorous, more pathogen resistant and more abiotic stress resistant. Thus, it presents an elegant and efficient way to improve plant productivity in vegetables and trees using traditional techniques. Despite this horticultural importance, we are only beginning to understand how plants regenerate tissues at the graft junction. By understanding grafting better, we can shed light on fundamental regeneration pathways and the basis for self/non‐self recognition. We can also better understand why many plants efficiently graft whereas others cannot, with the goal of improving grafting so as to broaden the range of grafted plants to create even more desirable chimeras. Here, I review the latest findings describing how plants graft and provide insight into future directions in this emerging field.

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Histological study of micrografting in passionfruit

Cited by 29 publications

References 25 publications

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Anatomy of Rootstocks and Scions in Four Pine Species

Plant grafting: insights into tissue regeneration

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