Histological studies on the development of porcine tonsils after birth

Yang, Yunhan; Jing, Yuchao; Wang, Jialu; Yang, Qian

doi:10.1002/jmor.20839

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Tissue samples were stained with hematoxylin and eosin and the data were measured by image‐pro Plus 6.0 (IPP) according to different parameters (area of PP, villus length in duodenum, jejunum, and ileum and crypt depth in colon). Each group was composed of 6 piglets, and 5 measurement units were randomly selected for each piglet, amounting to 30 measurement units in each group (Mou et al, ; Yang et al, ). Intestinal samples were observed under an optical microscope (PPs, intestinal villi and colonic crypt magnification x100; IEL magnification ×400).…”

Section: Methodsmentioning

confidence: 99%

4,4′‐Diaponeurosporene‐Producing Bacillus subtilis Promotes the Development of the Mucosal Immune System of the Piglet Gut

Jing

Liu

et al. 2019

The Anatomical Record

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Gut mucosal immune responses are known to act as the first line of defense against invasion of pathogenic microorganisms. Piglets have an incompletely developed gut mucosal immune system, making them sensitive to intestinal infections. Promoting the development of the mucosal immune system will increase the pathogen resistance of piglets. The aim of the present study was to investigate the effect of carotenoid (4,4′‐diaponeurosporene)‐producing Bacillus subtilis (B.s‐Dia) on intestinal mucosal immunity in piglets. We showed that oral administration to piglets of B.s‐Dia remarkably improved the development of Peyer's patches (PPs) (P < 0.01), and increased villus height (P < 0.01) and colon crypt depth (P < 0.01). In addition, B.s‐Dia also increased the number of intraepithelial lymphocytes (P < 0.01), while Bacillus subtilis (B.s) had no significant influence on it (P > 0.05). Moreover, B.s‐Dia also increased the number of SIgA+ cells (P < 0.01). Oral administration of either B.s or B.s‐Dia increased the number of CD4+ and CD8+ cells in ileum lamina propria (P < 0.01). These results indicate that B.s‐Dia contributes to a higher extent to porcine mucosal immune system development than B.s, and might serve as an immunopotentiator candidate. Anat Rec, 302:1800–1807, 2019. © 2019 American Association for Anatomy

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Section: Methodsmentioning

confidence: 99%

4,4′‐Diaponeurosporene‐Producing Bacillus subtilis Promotes the Development of the Mucosal Immune System of the Piglet Gut

Jing

Liu

et al. 2019

The Anatomical Record

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“…The lymphatic tissue of the tonsils has a cellular structure resembling that of lymph nodes, comprising B cell-containing follicles, germinal centers, and parafollicular areas dominated by T cells. Although structural development of the porcine tonsils rapidly proceeds after birth [20, 21], the earlier fetal component of tonsillar development has been poorly described.…”

Section: Introductionmentioning

confidence: 99%

Fetal and early postnatal development of the porcine tonsils of the soft palate

Suzuki

Fuchimoto

2019

Exp. Anim.

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Tonsils are mucosa-associated lymphoid tissues located at the openings of the gastrointestinal and respiratory tracts, which play a key role in the surveillance of inhaled or ingested pathogens and can concurrently be reservoirs of infectious agents. Therefore, tonsils are important for the immunology and hygiene management of domestic animals, including pigs. However, the process of their fetal developmental has been poorly described, at least in part, because rodents lack tonsils. Therefore, we performed a histological analysis of porcine tonsils of the soft palate from 60 to 100 days of gestation (DG) and from 2 to 14 days post partum (DP). This analysis showed that lymphoid aggregations first appear at DG65, gradually develop during the fetal stage, and expand after birth. In addition, the mRNA expression of chemokine genes involved in lymphoid aggregation and localization was analyzed. CCL19 expression showed the most marked increase and a sharp peak after birth. CCL21 expression changed moderately but showed an interesting bimodal pattern. CXCL13 expression steadily increased throughout the study period. Thus, we demonstrated the mRNA expression of chemokine characteristically changed accompanying tonsillar development.

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PEDV infection in neonatal piglets through the nasal cavity is mediated by subepithelial CD3+ T cells

Yuan

Jin

et al. 2021

Vet Res

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Porcine epidemic diarrhea virus (PEDV) primarily infects neonatal piglets causing catastrophic effects on the global pig farming industry. PEDV infects piglets through the nasal cavity, a process in which dendritic cells (DCs) play an important role. However, neonatal piglets have fewer nasal DCs. This study found that subepithelial CD3+ T cells mediated PEDV invasion through the nasal cavity in neonatal piglets. PEDV could replicate in the nasal epithelial cells (NECs) isolated from the nasal cavity of neonatal piglets. Infection of NECs with PEDV could induce antiviral and inflammatory cytokines at the late stage. The infected NECs mediated transfer of virus to CD3+ T cells distributed in the subepithelial of the nasal cavity via cell-to-cell contact. The infected CD3+ T cells could migrate to the intestine via blood circulation, causing intestinal infection in neonatal piglets. Thus, the findings of this study indicate the importance of CD3+T cells in the dissemination of PEDV from the nasal cavity to the intestinal mucosa in neonatal piglets.

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Histological studies on the development of porcine tonsils after birth

Cited by 3 publications

References 29 publications

4,4′‐Diaponeurosporene‐Producing Bacillus subtilis Promotes the Development of the Mucosal Immune System of the Piglet Gut

4,4′‐Diaponeurosporene‐Producing Bacillus subtilis Promotes the Development of the Mucosal Immune System of the Piglet Gut

Fetal and early postnatal development of the porcine tonsils of the soft palate

PEDV infection in neonatal piglets through the nasal cavity is mediated by subepithelial CD3+ T cells

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