Histological Staining by Tritiated Actinomycin D: A Useful Tool to Study Neoplastic Cell Heterogeneity and Estrogen Sensitivity of Mammary Tumors

Kiss, Róbert; Leclercq, G; Danguy, André

doi:10.1159/000226015

Cited by 4 publications

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“…Among these models, the MXT mouse neoplasm is subcutaneously transplantable and was initially devel oped by Watson et al [3]. It has been shown to contain subpopulations of neoplastic cells which react dif ferently to the same hormonal stimulus [4], MXT cell proliferation is significantly modulated by estradiol (E2) [5][6][7], progesterone (Pg) [8. 9).…”

Section: Introductionmentioning

confidence: 99%

Influence of Fetal Calf Serum in Combination with Pharmacological Doses of Progesterone or Estradiol on Proliferation and Cell Cycle Kinetics of Cultured Mammary Cancer Cells

Kiss

Launoit²,

Danguy³

et al. 1989

Oncology

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We describe an original method to monitor clonal cell density (hyperplasia) and the cell cycle kinetics of neoplastic cells simultaneously. We thus characterize the in vitro influence of two different types of fetal calf serum (FCS), defined as FCS-S and FCS-I, on the progesterone- or estradiol-induced effect on proliferation and cell cycle kinetic parameters of the MXT mouse and MCF-7 human mammary cancer cell lines. The two sera were treated with dextran-coated charcoal. The FCS-S serum showed a stimulatory influence on MXT and MCF-7 growth, whereas FCS-I was devoid of any clear-cut influence. The cells were cultured on glass coverslips, placed in Petri dishes containing either a control or a hormone-added medium, fixed for histology and submitted to the Feulgen reaction, which allows selective and quantitative (stoichiometric) staining of DN A. Proliferation and cell cycle kinetics were analyzed on the same sample of cells by means of the SAMBA 200 cell image processor. Our results show that steroid-mediated effects were dramatically modulated according to the type of serum used. Furthermore, they also show that pharmacological doses of progesterone or estradiol decrease MXT cell growth by at least two different mechanisms: the first is related to cell cycle kinetics, i.e. an inhibition of the cells into the S phase, while the second remains unknown but seems to be cell cycle independent. High dose estradiol, but not progesterone, induced the same inhibitory influence on the MCF-7 cells.

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Section: Introductionmentioning

confidence: 99%