Histological, oxidative and immune changes in response to 9,10-phenanthrenequione, retene and phenanthrene in <i>Takifugu obscurus</i> liver

Jiang, Shulun; Yang, Jian; Fang, Di‐An

doi:10.1080/10934529.2020.1744998

Cited by 10 publications

(4 citation statements)

References 41 publications

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“…At higher retene concentrations associated with teratogenicity, disruption of redox balance appears to play a key role in toxicity response. This is consistent with previous studies implicating oxidative stress in retene-induced toxicity ( Peixoto et al, 2019 ; Jiang et al, 2020 ). Whether effects of oxidative stress are responsible for retene-induced teratogenicity is still unclear, though the findings of this study support this hypothesis.…”

Section: Discussionsupporting

confidence: 94%

Concentration-response gene expression analysis in zebrafish reveals phenotypically-anchored transcriptional responses to retene

et al. 2022

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Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants and are associated with human disease. Canonically, many PAHs induce toxicity via activation of the aryl hydrocarbon receptor (AHR) pathway. While the interaction between PAHs and the AHR is well-established, understanding which AHR-regulated transcriptional effects directly result in observable phenotypes and which are adaptive or benign is important to better understand PAH toxicity. Retene is a frequently detected PAH in environmental sampling and has been associated with AHR2-dependent developmental toxicity in zebrafish, though its mechanism of toxicity has not been fully elucidated. To interrogate transcriptional changes causally associated with retene toxicity, we conducted whole-animal RNA sequencing at 48 h post-fertilization after exposure to eight retene concentrations. We aimed to identify the most sensitive transcriptomic responses and to determine whether this approach could uncover gene sets uniquely differentially expressed at concentrations which induce a phenotype. We identified a concentration-response relationship for differential gene expression in both number of differentially expressed genes (DEGs) and magnitude of expression change. Elevated expression of cyp1a at retene concentrations below the threshold for teratogenicity suggested that while cyp1a expression is a sensitive biomarker of AHR activation, it may be too sensitive to serve as a biomarker of teratogenicity. Genes differentially expressed at only non-teratogenic concentrations were enriched for transforming growth factor-β (TGF-β) signaling pathway disruption while DEGs identified at only teratogenic concentrations were significantly enriched for response to xenobiotic stimulus and reduction-oxidation reaction activity. DEGs which spanned both non-teratogenic and teratogenic concentrations showed similar disrupted biological processes to those unique to teratogenic concentrations, indicating these processes were disrupted at low exposure concentrations. Gene co-expression network analysis identified several gene modules, including those associated with PAHs and AHR2 activation. One, Module 7, was strongly enriched for AHR2-associated genes and contained the strongest responses to retene. Benchmark concentration (BMC) of Module seven genes identified a median BMC of 7.5 µM, nearly the highest retene concentration with no associated teratogenicity, supporting the hypothesis that Module seven genes are largely responsible for retene toxicity.

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Section: Discussionsupporting

confidence: 94%

Concentration-response gene expression analysis in zebrafish reveals phenotypically-anchored transcriptional responses to retene

et al. 2022

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“…PAHs originate from natural and anthropogenic sources. , Natural sources primarily originate from volcanic eruptions and forest fires; anthropogenic sources are the main PAHs contributors and primarily include oil refineries, incomplete fuel combustion, and the discharge of industrial effluents. , Parent PAHs (PPAHs) can undergo chemical or microbial transformations, leading to substituted PAHs (SPAHs), such as alkylated (APAHs), oxygenated (OPAHs), nitrated (NPAHs), and halogenated PAHs (XPAHs). , Anthropogenic emissions are the main sources of SPAHs; of them, NPAHs are originated mainly from fossil fuel combustion sources and OPAHs mainly from petroleum sources . The toxicity of SPAHs is comparable to or higher than that of their PPAHs. − For example, 1-nitropyrene (1-NPyr), a typical NPAH, is more carcinogenic than pyrene and has been classified as a group 2A carcinogen in humans by International Agency for Research on Cancer (IARC) . The carcinogenic potency of 6-nitrochrysene (6- N -Chr) is 10-fold higher than that of benzo[ a ]pyrene (BaPyr) .…”

Section: Introductionmentioning

confidence: 99%

Distribution and Risk Assessment of 80 Polycyclic Aromatic Compounds in Surface Water and Industrial Effluent in the Upper Yangtze River, China

Xiong,

Zhang,

Wang

et al. 2024

ACS EST Water

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Polycyclic aromatic compounds (PACs) are widely present in aquatic environments and have attracted considerable attention because of their potential carcinogenicity, teratogenicity, and mutagenicity. However, compared with that of the 16 Environmental Protection Agency (EPA) polycyclic aromatic hydrocarbons (PAHs), information on the occurrence and ecological risks of other PACs is scarce. In this study, a liquid− liquid extraction coupled gas chromatography−tandem mass spectrometry method was established for the simultaneous analysis of 80 PACs, including 23 parent PAHs (PPAHs) and 12 alkylated, 10 oxygenated, 8 nitrated, 18 halogenated, and 9 heterocyclic PAHs, in 15 surface water and 7 industrial effluent samples collected from the upper reaches of Yangtze River. The average concentration of total PACs in surface water was 1106.4 ng/L, with ΣHPAH (974.2 ng/L) > ΣPPAH (55.6 ng/L) > ΣAPAH (48.1 ng/L) > ΣNPAH (16.8 ng/L) > ΣOPAH (10.9 ng/L) > ΣXPAH (0.8 ng/L). The average concentration of total PACs in industrial effluent was 373 ng/L with ΣHPAH (294.1 ng/L) > ΣPPAH (37.2 ng/L) > ΣAPAH (23 ng/L) > ΣOPAH (10.9 ng/L) > ΣOPAH (6.7 ng/L) > ΣXPAH (1.1 ng/L). Notably, indole concentrations up to 6466.9 and 1616.6 ng/L were detected in surface water and industrial effluent, respectively. Ecological risk assessment using the risk quotient (RQ) showed that all surface water samples exhibited high risks (RQ > 1), mainly derived from indole, 1,4-naphthoquinone, and PPAHs with high ring numbers. Additionally, 4 surface water samples exhibited medium to high risks, and 10 exhibited low to medium risks based on the total toxicity equivalent (TEQ carc ) evaluation, with the main contribution from benzo[a]pyrene. The results of this study would provide valuable data to comprehensively assess the exposure levels and risks of PACs.

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“…Em um estudo com zebrafish (D. rerio) foi observado que houve um aumento na atividade da SOD em animais expostos ao FE nas concentrações de 1 mg/L, 1,79 mg L -1 , 3,16 mg L -1 , 5,62 mgL -1 e 10 mgL -1 ), entretanto sem alterações na expressão gênica do mesmo (Mai et al, 2019). Já em outro estudo, com o peixe baiacu Takifugu obscurus a atividade da SOD diminuiu no fígado dos animais expostos a 44,29 µg L −1 de FE por 96h (Jiang et al, 2020).…”

Section: Discussionunclassified

“…De maneira geral, observou-se aumento de LPO no fígado de E. marginatus expostos a alta concentração de FE em comparação aos outros tratamentos, enquanto as brânquias permaneceram sem alterações. Visto que danos lipídicos podem resultar em disfunção de órgãos e em morte celular (Costantini, 2014), este resultado é corroborado por Campos et al obscurus) apresentava sinais de inflamação hepática e atrofia celular (Jiang et al, 2020), demonstrando que este tecido pode ser um alvo da toxicidade do FE.…”

Section: Discussionunclassified

Avaliação dos efeitos neurotóxicos e antioxidantes do fenantreno em juvenis de Epinephelus marginatus (Perciformes: Serranidae)

Faria

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Histological, oxidative and immune changes in response to 9,10-phenanthrenequione, retene and phenanthrene in Takifugu obscurus liver

Cited by 10 publications

References 41 publications

Concentration-response gene expression analysis in zebrafish reveals phenotypically-anchored transcriptional responses to retene

Concentration-response gene expression analysis in zebrafish reveals phenotypically-anchored transcriptional responses to retene

Distribution and Risk Assessment of 80 Polycyclic Aromatic Compounds in Surface Water and Industrial Effluent in the Upper Yangtze River, China

Avaliação dos efeitos neurotóxicos e antioxidantes do fenantreno em juvenis de Epinephelus marginatus (Perciformes: Serranidae)

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