Histological observations on lymphomyeloid organs of the Antarctic fish Trematomus bernacchii (Teleostei: Nototheniidae)

Romano, Nicla; Baldassini, Maria Rosaria; Terribili, Francesca Renata; Abelli, Luigi; Mastrolia, Lucia; Mazzini, Massimo

doi:10.1007/s003000050200

Cited by 16 publications

(14 citation statements)

References 9 publications

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“…In juveniles and adult sea bass, the head kidney contains a high number of antibodyproducing cells and fewer T lymphocytes [5,8,41]. The observation of lymphocyte traffic from the thymus to the head kidney was observed during the histogenesis of the thymus in carp [1] and in sea bass by using the DLT15 antibody [8 and the present study].…”

Section: Other Lymphoid Organs and Gutmentioning

confidence: 77%

“…In sea bass, the immigration was seen at around 50 days p.f., when the organ started to became enriched with B cells [38] and TcRb þ T cells [present study]. The spleen is involved in haematopoiesis, although its early role is generally limited to erythropoiesis and thrombopoiesis [4], and in juveniles, it has a role as a secondary lymphoid organ [5]. TcRb þ cells start to appear in the head kidney and the spleen at around 50 days p.f.…”

Section: Other Lymphoid Organs and Gutmentioning

confidence: 93%

“…Immature T cells undergo random rearrangement of their TcR a and b chains leading to surface expression of clonotypic TcRs; however, this process requires at least three main stages of signal transduction through the pre-T cell receptor (pre-TcR) [28]. Although the intrathymic selection process is only partially understood in fish, interactions between developing thymocytes and stromal elements have been evidenced during ontogeny [5,11,16,25]. Essentially, to support the maturation process, the epithelial milieu must be efficiently differentiated, as shown in mammals [28,29], birds [30] and fish [11,31].…”

Section: Thymusmentioning

confidence: 99%

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Antigen-dependent T lymphocytes (TcRβ+) are primarily differentiated in the thymus rather than in other lymphoid tissues in sea bass (Dicentrarchus labrax, L.)

Romano

Caccia

Piergentili

et al. 2011

Fish & Shellfish Immunology

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Section: Other Lymphoid Organs and Gutmentioning

confidence: 77%

Section: Other Lymphoid Organs and Gutmentioning

confidence: 93%

Section: Thymusmentioning

confidence: 99%

See 1 more Smart Citation

Antigen-dependent T lymphocytes (TcRβ+) are primarily differentiated in the thymus rather than in other lymphoid tissues in sea bass (Dicentrarchus labrax, L.)

Romano

Caccia

Piergentili

et al. 2011

Fish & Shellfish Immunology

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“…Reticularepithelial cells and nurse cells housed in the cortical thymus constantly mediate the positive selection process (Ritter and Crispe 1992). In fish, evidence for both epithelial cell types has been presented: reticular epithelial cells in the cortex and nurse cells in a different compartment, the C/M border (Romano et al 1997b(Romano et al , 1999a.…”

Section: Thymusmentioning

confidence: 99%

Majority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)

et al. 2007

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Real-time polymerase chain reaction (PCR) and in situ hybridization analyses were performed to investigate the occurrence and distribution of T-lymphocytes expressing TcRbeta in intestine and lymphoid tissues of the bony fish, Dicentrarchus labrax (sea bass). Immunohistochemistry with the monoclonal antibody DLT15 (pan-T-cell marker) was carried out to compare the cytology, distribution and number of T-cells and TcRbeta+ cells in the various sampled lymphoid organs. The highest TcRbeta expression was revealed by real-time PCR in the thymus, with high levels also being found in the gut. In the thymus, DLT15+ and TcRbeta+ cell populations were concentrated in the cortex and TcRbeta+ cells were notably reactive at the cortical-medullary border, suggesting a specialized role of this region in thymocyte selection. The density of DLT15+ T-cells increased from the anterior to posterior intestine, whereas TcRbeta+ lymphocytes were more numerous in the middle intestine compared with other segments. The existence, in fish thymus, of a medulla and a cortex comparable with those of mammals is revealed by this study. The concentration of TcRbeta+ cells in the sea bass midgut also strongly suggests a special role of this intestinal segment in antigen-specific cellular immunity. The large population of TcRbeta(-)/DLT15+ T-cells in the posterior gut can probably be ascribed to the TcRgammadelta phenotype fraction.

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“…In the process of adaptation it appears likely that the immune system also underwent important changes, such as the capacity to elicit responses at low temperature, but only scarce information is available on the structural organisation and function of lymphoid organs in polar fishes (O'Neill, 1989;Romano et al, 1997a). The general organisation of the fish immune system involves lymphoid organs such as thymus, head and trunk kidney, spleen and MALT (skin, gills and gut) (Romano, 1998).…”

Section: Introductionmentioning

confidence: 98%

Adaptation of fish lymphomyeloid organs to polar water

Romano

Ceccariglia

Abelli

et al. 2004

Chemistry and Ecology

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Lymphomyeloid organs of three common Antarctic fish species, Trematomus bernacchii, Trematomus nicolai and Chionodraco hamatus, were analysed.Contrary to species living in temperate sea water, the thymus of polar fishes were flattened, incompletely lobated and scarcely distinguishable by normal histology into cortical and medullary regions. Functional regionalisation, however, was suggested by differences in the sizes of thymocytes from the outer to the inner thymus zone. Another particularity was observed in the thymus of Trematomus species: next to lymphocytes, numerous erythroid cells circulated and differentiated in the parenchyma. Only two main types of epithelial cells could be found by cytological analysis: (i) limiting cells that surround the haematopoietic tissue and (ii) reticular cells that constitute the frame where the lymphoid and erythroid cells can proliferate and differentiate. The reticular cells could not be distinguished in cortical and medullary subtypes as observed in temperate-water fish. Numerous Hassall's corpuscles, probably with a scavenging role, were also observed in the thymus.The head kidney housed haematopoietic tissue, lacked any excretory tubules, and had a huge blood supply, characteristic of polar fish species. It appeared mainly lymphopoietic in C. hamatus but contemporary erythropoietic and lymphopoietic in Trematomus species. The ultrastructural analysis revealed the presence of both reticular and limiting epithelial cells. Reticular epithelial cells (REC) characteristically showed numerous vesicles with a granular content and cell debris. Numerous lymphoblasts, lymphocytes and plasma cells were observed among the REC. Erythropoiesis occurred in all polar species analysed, but in C. hamatus the erythroblasts did not differentiate because they had a fast senescence.The spleen appeared mainly erythropoietic, with scarcely developed areas of white pulp, in Trematomus species; the erythropoiesis was scarcely evident in C. hamatus. Small vascular ellipsoids showed numerous melano-macrophages in Trematomus, while large haematopoietic areas were organised around the capillaries in C. hamatus. Utrastructural analysis revealed, in all species examined, two main types of epithelial cells: reticular, close to the ellipsoids, and limiting-subcapsular, which surround the organ. A large blood supply and extended capillary frame were also observed in polar species. The possible adaptation of lymphoid organs to the low temperatures of polar water is discussed.

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Histological observations on lymphomyeloid organs of the Antarctic fish Trematomus bernacchii (Teleostei: Nototheniidae)

Cited by 16 publications

References 9 publications

Antigen-dependent T lymphocytes (TcRβ+) are primarily differentiated in the thymus rather than in other lymphoid tissues in sea bass (Dicentrarchus labrax, L.)

Antigen-dependent T lymphocytes (TcRβ+) are primarily differentiated in the thymus rather than in other lymphoid tissues in sea bass (Dicentrarchus labrax, L.)

Majority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)

Adaptation of fish lymphomyeloid organs to polar water

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