Histological investigation of osteoinductive properties of rh-BMP2 in a rat calvarial bone defect model

Inoda, Hirosato; Yamamoto, Go; Hattori, Takanori

doi:10.1016/j.jcms.2004.06.003

Cited by 41 publications

(38 citation statements)

References 19 publications

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“…Inoda et al 18 reported that following implantation of rhBMP-2 with collagen in the rat cervical model, most of the collagen had disappeared 6 weeks after implantation, and that all of the atelopeptide type 1 collagen sponge had been reabsorbed and substituted by mature bone 9 weeks after implantation.…”

Section: Discussionmentioning

confidence: 99%

Statin-induced bone morphogenetic protein (BMP) 2 expression during bone regeneration: an immunohistochemical study

Alam

Ueki

Nakagawa

et al. 2009

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

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Objectives: The purpose of this study was to investigate bone morphogenetic protein (BMP)-2 expression following implantation of a statin and recombinant human BMP-2 (rhBMP-2), and to compare the bone regeneration capability of these substances in the rabbit nasal bone using immunohistological methods. Study design:Twelve adult male Japanese white rabbits (n = 12, age: 12-16 weeks, weight: 2.5-3.0 kg) were divided into three experimental groups and one control group.A total of 48 bone defects, four per rabbit, were created in the nasal bone while preserving the nasal membrane. In the experimental groups, one group was implanted with 10 mg statin dissolved in 0.2 ml water with an atelocollagen sponge (ACS), the second group was implanted with 5 μg rhBMP-2 with an ACS, and in the third group only the ACS was implanted. No material was implanted in the control group.Animals were killed at 1-, 2-, and 4-weeks postoperatively. The parts that had been operated on were removed and prepared for histological assessment. The expression of BMP-2 was evaluated using immunohistochemistry, and double-immunostaining for BMP-2 and Ki67 was observed by fluorescent microscopy.2

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Section: Discussionmentioning

confidence: 99%

Statin-induced bone morphogenetic protein (BMP) 2 expression during bone regeneration: an immunohistochemical study

Alam

Ueki

Nakagawa

et al. 2009

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

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show abstract

“…This is an extremely important property of scaffolds used for bone regeneration. It has been reported in previous studies that type I collagen had been resorbed and substituted by mature bone 9 weeks after implantation [25]. The authors of that study concluded that this histological sequence was ideal for induction of osteogenesis.…”

Section: Discussionmentioning

confidence: 87%

Evaluation of the ability of collagen–glycosaminoglycan scaffolds with or without mesenchymal stem cells to heal bone defects in Wistar rats

Alhag

Farrell

Toner

et al. 2011

Oral Maxillofac Surg

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“…Previous studies also showed that parietal bone defects measuring 2 mm in diameter in 12-wk-old Wistar rats and 4-mm-diameter defects in 28-d-old Wistar rats persisted for up to 6 mo after surgery (Freeman and Turnbull 1973;Mulliken and Glowacki 1980). Therefore, we created a critical bone defect with a diameter of 4 mm, which is the most suitable size for Wistar rats (Hollinger and Kleinschmidt 1990;Inoda et al 2004). One mo after transplantation of disc made from the engineered 3-D tissue in the CG-RC system, sparse osteogenesis and osteoid formation was observed.…”

Section: Resultsmentioning

confidence: 94%

Transplantation of engineered bone tissue using a rotary three-dimensional culture system

Hidaka

Chen

et al. 2007

In Vitro Cell.Dev.Biol.-Animal

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Bone is a complex, highly structured, mechanically active, three-dimensional (3-D) tissue composed of cellular and matrix elements. We previously published a report on in situ collagen gelation using a rotary 3-D culture system (CG-RC system) for the construction of large tissue specimens. The objective of the current study was to evaluate the feasibility of bone tissue engineering using our CG-RC system. Osteoblasts from the calvaria of newborn Wistar rats were cultured in the CG-RC system for up to 3 wk. The engineered 3-D tissues were implanted into the backs of nude mice and calvarial round bone defects in Wistar rats. Cell metabolic activity, mineralization, and bone-related proteins were measured in vitro in the engineered 3-D tissues. Also, the in vivo histological features of the transplanted, engineered 3-D tissues were evaluated in the animal models. We found that metabolic activity increased in the engineered 3-D tissues during cultivation, and that sufficient mineralization occurred during the 3 wk in the CG-RC system in vitro. One mo posttransplantation, the transplants to nude mice remained mineralized and were well invaded by host vasculature. Of particular interest, 2 mo posttransplantation, the transplants into the calvarial bone defects of rats were replaced by new mature bone. Thus, this study shows that large 3-D osseous tissue could be produced in vitro and that the engineered 3-D tissue had in vivo osteoinductive potential when transplanted into ectopic locations and into bone defects. Therefore, this system should be a useful model for bone tissue engineering.

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Histological investigation of osteoinductive properties of rh-BMP2 in a rat calvarial bone defect model

Cited by 41 publications

References 19 publications

Statin-induced bone morphogenetic protein (BMP) 2 expression during bone regeneration: an immunohistochemical study

Statin-induced bone morphogenetic protein (BMP) 2 expression during bone regeneration: an immunohistochemical study

Evaluation of the ability of collagen–glycosaminoglycan scaffolds with or without mesenchymal stem cells to heal bone defects in Wistar rats

Transplantation of engineered bone tissue using a rotary three-dimensional culture system

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