Histological features of peritoneal lavage with ropivacaine in rats with fecal peritonitis

PURPOSE:To evaluate the treatment outcome of severe peritonitis in rats submitted to permanent bilateral carotid occlusion (PBCO). METHODS:Sixteen Wistar rats (mean age of 8.5 months) with PBCO underwent autogenously fecal peritonitis, and were treated with moxifloxacin combined with dexamethasone, and followed-up for 45 days. Ten rats (mean age five months) without PBCO were used as a control group. The variables were expressed by their mean and standard error of the mean (SEM). p<0.05 was used for rejecting the null hypothesis. The study was approved by the Ethics Committee. RESULTS:There was a significant increase (p=0.0002) in the mortality and morbidity in older rats that underwent PBCO (study group). However, even among the survival rats presenting with severe residual abscesses both in the abdomen and thorax cavities, they present an almost normal life. CONCLUSIONS:The treatment of severe autogenously fecal peritonitis with intraperitoneal moxifloxacin combined with dexamethasone was very effective in young rats without permanent bilateral carotid occlusion. The treatment reached reasonable results in older rats with PBCO, even considering residual abscesses on abdomen and thorax. Older age was the greater risk factor for the outcome of the treatment of severe peritonitis. Sepsis remains a challenging situation, especially in elderly.Key words: Peritonitis. Quinolones. Dexamethasone. Therapy. Rats. Severe autogenously fecal peritonitis in Wistar rats with permanent bilateral carotid occlusion.Response to intra peritoneal moxifloxacin combined with dexamethasone

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“…Furthermore, the follow-up, post intraperitoneal treatment, in the present study was longer than others performed in mice 31 and rats 32 .…”

Section: Discussionmentioning

confidence: 57%

“…Additionally, the follow-up is usually short in time, and there is no mention to mean age of the animals [29][30][31][32] . Almost all of these studies lend no support to concept that antibiotics or others substances produce reduction of the morbidity and mortality for sepsis secondary to peritonitis 9,30 .…”

Section: Discussionmentioning

confidence: 99%

Severe autogenously fecal peritonitis in Wistar rats with permanent bilateral carotid occlusion. Response to intra peritoneal moxifloxacin combined with dexamethasone

Melo

Gadelha²,

Oliveira

et al. 2014

Acta Cir. Bras.

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“…Although this outcome would be expected, it is necessary to assess the used model as regard to elderly rats. The usual dosage of autogenously 10% fecal suspension for testing therapeutically intervention is 5 ml/kg 6,21 . In these papers the rats included in the investigation weighed 300g to 330g, however there was no mention to the ages of the rats in some studies 5,6 , and in other the rats were very young (between two and three months).…”

Section: Discussionmentioning

confidence: 99%

“…intraabdominal sepsis in rat model describe the animals as adult male, Wistar-albino rats weighing from 250 to 320 g. However, chronological ages are not specified in most of the studies [5][6][7] . On the other hand, some studies used very young Wistar rats (age between two and three months, and weight between 200 and 250g) 8 .…”

Section: Several Interventional Approaches For Treatingmentioning

confidence: 99%

Severe autogenously fecal peritonitis in ageing Wistar rats. Response to intravenous meropenem

Mascena

Melo²,

Gadelha³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To evaluate the treatment outcome of severe peritonitis in rats with increasing age. METHODS:Thirty Wistar rats stratified in three groups: group I -six month-old; group II -12 month-old; and group III -18 monthold, underwent autogenously fecal peritonitis (6 ml/kg rat), and were treated with intravenous meropenem. The survival animals were followed-up for 45 days. The variables were expressed by their mean and standard error of the mean (SEM). p<0.05 was used for rejecting the null hypothesis. The study was approved by the Ethics Committee. RESULTS:There was a significant increase in the mortality and morbidity in elderly rats. Of interest, even among young survival rats presenting with severe residual abscesses both in the abdomen and thorax cavities, they present an almost normal life. CONCLUSIONS:The treatment of severe autogenously fecal peritonitis with intravenous meropenem reached reasonable results in rats with six and twelve months of age, even considering residual abscesses on abdomen and thorax cavities. However, the great majority (80%) of elderly rats could not overcome the initial severe infectious challenge, proving that ageing is a very important risk factor for impairing immune response. Thus, sepsis remains a challenging situation, especially in elderly.

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“…In this regard, several substances have been used aiming at controlling or modulating the inflammatory cascade that is produced after peritonitis occurs. The results seem to be acceptable 16,17 however the main goal for treating severe intra-abdominal infection is not dealt in a rational way -it means not battling the overgrow of microorganisms in this cavity or in blood stream.…”

Section: Alcohol Extract Of Schinu Sterebinthifolius Raddi (Anacardiamentioning

confidence: 99%

Alcohol extract of Schinu sterebinthifolius raddi (anacardiaceae) as a local antimicrobial agent in severe autogenously fecal peritonitis in rats

Melo

Gadelha²,

Oliveira

et al. 2014

Acta Cir. Bras.

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PURPOSE:To develop an alcoholic extract of the inner bark of the Schinus terebinthifolius raddi and to test its impact on autogenously fecal peritonitis in Wistar rats. METHODS:The inner bark of the Schinus terebinthifolius raddi was kept for seven days in 70% ethanol alcohol. The total elimination of the solvent was performed in a rotary evaporator under reduced pressure at 55-60°C. Four milliliter of this extract was injected, after 24 h, into the abdominal cavity of six out of eight survival rats that underwent autogenously fecal peritonitis with five milliliter of 10% filtered fecal suspension. They were clinically followed up for 45 days when they were euthanized. The necropsy findings (inventory) of the abdominal and thorax cavities were inspected and the main findings were recorded and photographed. The investigation was approved by the Ethics Committee. RESULTS:Two out of six survival rats that were critically ill after 24 h died within the 12 h after the extract injection into the abdominal cavity. Four rats that were also critically ill recovered and gradually became healthy, eating well, regaining weight and moving normally in the cage. At 45 days post severe peritonitis the necropsy findings revealed few signs of residual infection on the abdominal and thorax cavities. There were no bowel adhesions. CONCLUSION:The impact of alcoholic extract of the inner bark of the Schinus terebinthifolius raddi was considered very positive and promising as natural local antiseptic against very severe peritonitis in Wistar rats.

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Histological features of peritoneal lavage with ropivacaine in rats with fecal peritonitis

Cited by 11 publications

References 17 publications

Severe autogenously fecal peritonitis in Wistar rats with permanent bilateral carotid occlusion. Response to intra peritoneal moxifloxacin combined with dexamethasone

Severe autogenously fecal peritonitis in Wistar rats with permanent bilateral carotid occlusion. Response to intra peritoneal moxifloxacin combined with dexamethasone

Severe autogenously fecal peritonitis in ageing Wistar rats. Response to intravenous meropenem

Alcohol extract of Schinu sterebinthifolius raddi (anacardiaceae) as a local antimicrobial agent in severe autogenously fecal peritonitis in rats

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