Histological Evidences of Autograft of Dentin/Cementum Granules into Unhealed Socket at 5 Months after Tooth Extraction for Implant Placement

Abstract

The aim of this clinical case study was to observe biopsy tissues at 5 months after an autograft of a partially demineralized dentin/cementum matrix (pDDM) into a tooth-extracted socket exhibiting healing failure. A 66-year-old female presented with healing failure in the cavity for 2 months after the extraction (#36). Initial X-ray photos showed a clear remainder of lamina dura (#36), a residual root (#37), and a horizontal impaction (#38). The vital tooth (#38) was selected for pDDM. The third molar crushed by electric mill was decalcified in 1.0 L of 2.0% HNO3 for 20 min and rinsed in cold distilled water. The pDDM granules (size: 0.5–2.0 mm) were grafted immediately into the treated socket. X-ray views just after pDDM graft showed radio-opaque granules. At 5 months after pDDM graft, the surface of regenerated bone was harmonized with the mandibular line, and bone-like radio-opacity was found in the graft region. The biopsy tissue (diameter: 3.0 mm) at 5 months after pDDM graft showed that mature bone was interconnected with the remaining pDDM. The novel histological evidence highlighted that newly formed bone was connected directly with both dentin-area and cementum-area matrix of pDDM. We concluded that pDDM contributed to the regeneration of bone in the unhealed socket, and this regeneration prepared the socket for implant placement. Autogenous pDDM could be immediately recycled as an innovative biomaterial for local bone regeneration.

Adipose cell induction under the impacted tooth and extraction facilities (Clinical therapy)

“…Guidelines from the United Kingdom, Australia, and North America indicate an INR between 2 and 3 or between 2.5 and 3.5 for mechanical heart valves [24][25][26] . Patients diagnosed with non-valvular atrial fibrillation often get prescriptions for oral anticoagulants as a means of mitigating their risk of developing thromboembolic complications [27][28][29] . Patients who have had adipose tissue transplanted do not need regular coagulation monitoring or lab-based dose changes; instead, the proper dosage is established by the patient's GFR (GFR) [30][31][32] .…”

“…Because of this, the anticoagulant drug you take may not need to be as strong. In order to provide better care for those who have received organ transplants or adipose tissue, the medical and nursing sectors both need further training as well as fresh ideas [27][28][29] . This study isn't perfect; for example, there are charts involved, and access to the data is limited [30][31][32] .…”

Treated Dentin Matrix in Tissue Regeneration: Recent Advances

“…Extracted human teeth are the most common source for harvesting TDM raw material [ 43 , 46 , 49 , 50 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ]. Experiments conducted with animal TDMs, such as bovine [ 43 , 48 ], canine [ 28 ], goat [ 49 ], monkey [ 28 ] and porcine TDMs [ 28 , 31 , 32 , 46 , 66 , 67 , 68 ], show that these are also feasible.…”

“…Treated-dentin-matrix-based bio-material’s application in bone tissue regeneration mainly focuses on alveolar ridge preservation and socket preservation after extraction [ 62 , 64 , 85 , 86 , 87 ]. HE staining, Masson staining and immunostaining are commonly used to verify the mineralized tissue formation.…”

Research on the clinical application of autogenous tooth bone graft material

“…In 2003, some scholars reported for the first time the successful case of clinical application of autogenous tooth and bone meal in maxillary sinus lifting [21]. In 2007, Japanese scholars invented tooth grinding machine, which made the production of autogenous tooth and bone meal more convenient [22]. In 2009, the Korea Tooth Bank was established to collect and store teeth and prepare them into bone grafts [23].…”