Histological Evaluation to Study the Effects of Dental Amalgam and Composite Restoration on Human Dental Pulp: An in vivo Study

Liu

et al. 2019

IJMS

Dental caries is a common disease on a global scale. Resin composites are the most popular materials to restore caries by bonding to tooth tissues via adhesives. However, multiple factors, such as microleakage and recurrent caries, impair the durability of resinous restorations. Various innovative methods have been applied to develop adhesives with particular functions to tackle these problems, such as incorporating matrix metalloproteinase inhibitors, antibacterial or remineralizing agents into bonding systems, as well as improving the mechanical/chemical properties of adhesives, even combining these methods. This review will sum up the latest achievements in this field.

Section: The Factors Related With the Failure Of Resin-dentin Bondingmentioning

confidence: 99%

Modifying Adhesive Materials to Improve the Longevity of Resinous Restorations

Liu

et al. 2019

IJMS

“…25 Based on the results of the present study, there was no significant difference in patient preference in relation to which material was used. So, the clinicians should make amalgam restoration a restorative option for many reasons, beginning with the longevity of this material and lesser cost depending on patient affordability 26 and it is biologically compatibility. 27 The availability of silver amalgam capsules in the workplace was not sufficient so the study was on hold for some time.…”

Section: Discussionmentioning

confidence: 99%

Measurement of patient satisfaction with two different restorative materials in non esthetic zone area by using OHIP5: Prospective study

Alzunaydi

Alsalem

Aldhuwayhi

2022

JPTCP

Background: Many dental restorative materials are used in dental clinics, while in a new practice, many countries are trying to ban dental amalgam for many reasons. Dental mercury is the main issue for suspending the use of dental amalgam. Another restoration method, the composite restoration for posterior and anterior teeth for esthetic porous, became the alternative to amalgam. Aim: To measure patient satisfaction with two different materials based on multiple criteria using an oral health impact profile (OHIP) form. Method: This is a prospective study on two groups. The patients visiting the clinic with a vital posterior tooth indicated for restoration were requested to participate in the study. The first group received composite restoration of the posterior teeth. Contrarily, the second group underwent an amalgam restoration application. Patient satisfaction was assessed using the OHIP5 to assess different aspects of patient satisfaction. The patients were asked to fill out a form before starting the procedure, and after 4 weeks, the procedure was reported. The operators were requested to fill out their forms based on the procedure done to determine the participant eligibility criteria. Results: Overall 64 subjects were involved in the study among them 35 participants who received composite restoration, 48.5% were female, whereas 51.5% were male. Under other conditions, the patients who underwent amalgam application were 29, and 41.4% were female. Based on the study results, the participants underwent before and after assessment and showed no demands for different aspects with the two different materials.

“…Monomers have been shown to reach the pulp, with 4 mM being quoted as a worst-case intrapulpal concentration [ 15 ]. Clinical observations suggest that monomer-containing adhesives/RBCs may lead to pulp inflammation [ 12 , 13 ] and MMP induction [ 23 ]. Pulp-derived MMPs have been suggested to play a role in caries [ 2 ], hybrid layer degradation [ 14 ], as well as pulpal inflammation [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…Resin-based composites (RBC) and their respective bonds are a mixture of resin monomers polymerized by an appropriate wavelength of light. RBC restorations and especially the application of adhesives have been associated with pulp inflammation and higher secondary caries rate, when compared with amalgam [12][13][14]. Current material designs rarely en-sureaconversiondegreeexceeding75%,resultinginthepresence of leachable monomers such as triethylene-glycol dimethacrylate (TEGDMA), which can reach the pulp [15].…”

Section: Introductionmentioning

confidence: 99%

Influence of TEGDMA monomer on MMP-2, MMP-8, and MMP-9 production and collagenase activity in pulp cells

et al. 2020

Objectives Resin-based composites may leach monomers such as triethylene-glycol dimethacrylate (TEGDMA), which could contribute to intrapulpal inflammation. The aim of this investigation was to examine whether various concentrations of TEGDMA are able to influence dentally relevant Matrix metalloproteinase (MMP)-2, MMP-8, and MMP-9 production, total collagenase/gelatinase activity in pulp cells, and suggest possible signaling mechanisms. Materials and methods Pulp cells were cultured, followed by a 1-day exposure to sublethal TEGDMA concentrations (0.1, 0.2, and 0.75 mM). Total MMP activity was measured by an EnzCheck total collagenase/gelatinase assay, while the production of specific MMPs and the relative changes of phosphorylated, i.e., activated signaling protein levels of extracellular signal-regulated kinase (ERK)1/2, p38, c-Jun N-terminal kinase (JNK) were identified by western blot. Immunocytochemistry image data was also plotted and analyzed to see whether TEGDMA could possibly alter MMP production. Results An increase in activated MMP-2, MMP-8, and MMP-9 production as well as total collagenase activity was seen after a 24-h exposure to the abovementioned TEGDMA concentrations. Increase was most substantial at 0.1 (P = 0.002) and 0.2 mM (P = 0.0381). Concurrent pERK , p-p38, and p-JNK elevations were also detected. Conclusions Results suggest that monomers such as TEGDMA, leached from resin-based restorative materials, activate and induce the production of dentally relevant MMPs in pulp cells. Activation of ERK1/2, p38, or JNK and MMP increase may play a role in and/or can be part of a broader stress response. Clinical relevance Induction of MMP production and activity may further be components in the mechanisms of intrapulpal monomer toxicity.