Histological evaluation of vital organs of the livebearer Jenynsia multidentata (Jenyns, 1842) exposed to glyphosate: A comparative analysis of Roundup® formulations

Sánchez, Jessica Andrea Albañil; Klosterhoff, Marta da Costa; Romano, Luís Alberto; Martins, Camila de Martinez Gaspar

doi:10.1016/j.chemosphere.2018.11.020

Cited by 22 publications

(6 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lesions observed in the liver of Caucasian dwarf gobies are in agreement with the findings from other studies, which tested the effects of different pollutants on fish (Greenfield et al, ; Hued, Oberhofer, & de los Angeles Bistoni, ; Kaoud & El‐Dahshan, ; Samanta, Mukherjee, Pal, Kole, & Ghosha, ). Our results confirmed those of Cuevas et al () and Albañil Sánchez et al () according to whom lipid degeneration is characterized by an increase in the synthesis or accumulation of fats in the form of vacuoles. We consider that the large amount of lipids results in an increased triglyceride synthesis and hyperlipidemia associated with fatty infiltration of hepatocytes.…”

Section: Discussionsupporting

confidence: 93%

“…We share the opinion of Coz‐Rakovac et al () and Marchand, van Dyk, Pieterse, Barnhoorn, and Bornman () that because of the liver's important functions, multiple hepatic lesions are to be expected in wild fish populations. We agree with Albañil Sánchez, da Costa Klosterhoff, Romano, and De Martinez Gaspar Martins () that the liver concentrates a large portion of toxicants, which can eventually damage the organ. Therefore, the hepatic alterations may indicate that this organ is overloaded, and at the same time it can be an alert for a possible failure of the liver, which would impair the detoxification capacity of the fish.…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

A histopathological study on the Caucasian dwarf goby from an anthropogenically loaded site in Hungary using multiple tissues analyses

et al. 2020

View full text Add to dashboard Cite

The present study aimed to investigate for the first time the health status of the Caucasian dwarf goby Knipowitschia caucasica (Berg, 1916, Fishes of freshwaters of Russian Empire, p. 563, Moscow, Russia: Dep. Zemledeliya) (Teleostei: Gobiidae) from an anthropogenically loaded site in Hungary using histopathological analyses on multiple tissues. For that purpose, fish were collected from the public beach at Tiszafüred near the River Tisza. Gills, liver and kidney were subjected to histopathological analyses, and the results showed different alterations in each organ, which also differed in their extent and severity. In addition, we also found lesions in the reproductive organs of both, male and female fish which, overall, we hypothesized could be due to untreated municipal wastewaters, most likely contaminated with endocrine‐disrupting chemicals. The multi‐organ histopathological analyses of Caucasian dwarf gobies revealed different lesions, prevalence and severity in each target organ, as follows: liver>gills>kidney>gonad (testes and ovaries). These histopathological lesions can be assessed as good indicators of contamination by endocrine‐disrupting chemicals of freshwater ecosystems.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

A histopathological study on the Caucasian dwarf goby from an anthropogenically loaded site in Hungary using multiple tissues analyses

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Many co-formulants are more toxic than technical glyphosate [31,36] and synergistic activity may occur in some exposure scenarios with certain formulations. Accordingly, differential toxicity arises from variable combinations of the innate toxicity of the surfactant(s) in a GBH compared to technical glyphosate, the impact of the surfactant(s) on the movement of glyphosate through human skin and into cells, and possible synergistic impacts [30,[37][38][39].…”

Section: Differential Toxicitymentioning

confidence: 99%

How did the US EPA and IARC reach diametrically opposed conclusions on the genotoxicity of glyphosate-based herbicides?

Benbrook

2019

Environ Sci Eur

134

View full text Add to dashboard Cite

Background: The US EPA considers glyphosate as "not likely to be carcinogenic to humans. " The International Agency for Research on Cancer (IARC) has classified glyphosate as "probably carcinogenic to humans (Group 2A). " EPA asserts that there is no convincing evidence that "glyphosate induces mutations in vivo via the oral route. " IARC concludes there is "strong evidence" that exposure to glyphosate is genotoxic through at least two mechanisms known to be associated with human carcinogens (DNA damage, oxidative stress). Why and how did EPA and IARC reach such different conclusions? Results: A total of 52 genotoxicity assays done by registrants were cited by the EPA in its 2016 evaluation of technical glyphosate, and another 52 assays appeared in the public literature. Of these, one regulatory assay (2%) and 35 published assays (67%) reported positive evidence of a genotoxic response. In the case of formulated, glyphosatebased herbicides (GBHs), 43 regulatory assays were cited by EPA, plus 65 assays published in peer-reviewed journals. Of these, none of the regulatory, and 49 published assays (75%) reported evidence of a genotoxic response following exposure to a GBH. IARC considered a total of 118 genotoxicity assays in six core tables on glyphosate technical, GBHs, and aminomethylphosphonic acid (AMPA), glyphosate's primary metabolite. EPA's analysis encompassed 51 of these 118 assays (43%). In addition, IARC analyzed another 81 assays exploring other possible genotoxic mechanisms (mostly related to sex hormones and oxidative stress), of which 62 (77%) reported positive results. IARC placed considerable weight on three positive GBH studies in exposed human populations, whereas EPA placed little or no weight on them. Conclusions: EPA and IARC reached diametrically opposed conclusions on glyphosate genotoxicity for three primary reasons: (1) in the core tables compiled by EPA and IARC, the EPA relied mostly on registrant-commissioned, unpublished regulatory studies, 99% of which were negative, while IARC relied mostly on peer-reviewed studies of which 70% were positive (83 of 118); (2) EPA's evaluation was largely based on data from studies on technical glyphosate, whereas IARC's review placed heavy weight on the results of formulated GBH and AMPA assays; (3) EPA's evaluation was focused on typical, general population dietary exposures assuming legal, food-crop uses, and did not take into account, nor address generally higher occupational exposures and risks. IARC's assessment encompassed data from typical dietary, occupational, and elevated exposure scenarios. More research is needed on real-world exposures to the chemicals within formulated GBHs and the biological fate and consequences of such exposures.

show abstract

“…In addition, the production of free radicals, lipid peroxidation and changes in the antioxidant state are vital factors for the toxic effects of pesticides on the liver histological structure [63]. According to Albañil Sánchez et al [45], lipid degeneration in fish liver caused by pesticides leads to dysfunctions in the microsomal and mitochondrial systems of cell, which can lead to the inhibition of the synthesis of lipoproteins. As a result of both acute and chronic exposure to pesticides, the hepatic function is affected, resulting in a reduction of protein concentration [64], which, in turn, affects the liver tissue.…”

Section: Discussionmentioning

confidence: 99%

“…The experimental set up was performed in triplicate. The tested insecticide was added only at the beginning of the experiment and the water was not renewed, as explained in other studies [43][44][45]. In addition, the half-life of CPF in water ranges from 3.5 to 20 days and 35 to 78 days, depending on the pH, as described above; therefore, it was supposed that the concentrations of CPF would not decrease considerably during the present experimental design of 72 h. No control with cyclohexane only was used, as its concentration was extremely low and no effect was expected.…”

Section: Animals and Treatmentsmentioning

confidence: 99%

Multi-Biomarker Assessment in Common Carp (Cyprinus carpio, Linnaeus 1758) Liver after Acute Chlorpyrifos Exposure

Stoyanova

Georgieva

Velcheva

et al. 2020

Water

View full text Add to dashboard Cite

The excessive use of pesticides at different stages of crop production can pose a great danger to the aquatic environment, and particularly to fish. The purpose of the present work was to assess the negative effects of chlorpyrifos (CPF) on the liver histological architecture and the activities of marker enzymes in common carp (Cyprinus carpio Linnaeus, 1758), by applying a multi-biomarker technique. The tested insecticide is categorized as a priority pollutant in surface waters in terms of Directive 2013/39/EU. The carps were exposed to different and environmentally relevant CPF concentrations for 72 h (a short-term acute experiment). The results showed that the tested insecticide alters the liver histological structure, causing degenerative lesions, such as granular and vacuolar degeneration; necrobiotic alterations and necrosis, as well as changes in the circulatory system. In addition, CPF induces changes in the enzymatic activity of lactate dehydrogenase (LDH), aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), cholinesterase (ChE), glutathione peroxidase (GPx) and catalase (CAT). The results from such experimental set ups could be successfully used in the legislation related to the protection of water bodies from contamination, in areas with intensive application of plant protection products used in agricultural practices, and also in implementing the Water Frame Directive by using multi-biomarker approaches.

show abstract

Histological evaluation of vital organs of the livebearer Jenynsia multidentata (Jenyns, 1842) exposed to glyphosate: A comparative analysis of Roundup® formulations

Cited by 22 publications

References 52 publications

A histopathological study on the Caucasian dwarf goby from an anthropogenically loaded site in Hungary using multiple tissues analyses

A histopathological study on the Caucasian dwarf goby from an anthropogenically loaded site in Hungary using multiple tissues analyses

How did the US EPA and IARC reach diametrically opposed conclusions on the genotoxicity of glyphosate-based herbicides?

Multi-Biomarker Assessment in Common Carp (Cyprinus carpio, Linnaeus 1758) Liver after Acute Chlorpyrifos Exposure

Contact Info

Product

Resources

About