Histological criteria for the identification of acute cellular rejection in human small bowel allografts: results of the pathology workshop at the VIII International Small Bowel Transplant Symposium

Ruiz, Phillip; Bagni, Alberto; Brown, Rachel M.; Cortina, Galen; Harpaz, Noam; Magid, Margret S.; Reyes, Jorgé

doi:10.1016/j.transproceed.2004.01.079

Cited by 189 publications

(123 citation statements)

References 5 publications

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“…Tx patient data are summarized in Table 1a. Histological evaluations of biopsy specimens were conducted by trained pathologists using the classical rejection grading system (22). The second group (non-Tx group) included samples obtained from 10 non-Tx patients (two children and eight adults) undergoing other abdominal surgical procedures from which a distal ileum segment was obtained and used as healthy control group (Table 1b).…”

Section: Patients and Study Groupsmentioning

confidence: 99%

Immunological Status of Isolated Lymphoid Follicles After Intestinal Transplantation

Meier

Docena

Ramisch

et al. 2014

American Journal of Transplantation

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Intestinal transplantation (ITx) faces the challenge of grafting a high immunogenic organ, which is certainly one of the major obstacles for intestinal allograft acceptance. The allograft has to guarantee the proper functioning of the mucosal immune machinery under immunosuppressive conditions. Recently, it has been elucidated that isolated lymphoid follicles (ILFs) are an indispensable part of mucosal immunity to maintain IgA synthesis and consequently to control commensal microflora. No data about these follicular structures in the setting of ITx are available so far. Therefore, we addressed the question whether constitution, integrity and function of allograft ILFs are disturbed by immunosuppressive regimen. We compared allograft ILFs from terminal ileum of transplant patients with ILFs from nontransplant patients via flow cytometry, quantitative real-time polymerase chain reaction and immunohistochemistry. We found that host leukocytes rapidly repopulate allograft ILFs and that maintenance immunosuppressive regimen, tacrolimus and corticosteroids, does not affect their cellular integrity and function. However, allograft ILFs revealed a higher maturation state than control samples and IgA positive plasma cells were increased in number in allograft mucosa. Our results open the path for a better understanding of allograft mucosal immunity.

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Section: Patients and Study Groupsmentioning

confidence: 99%

Immunological Status of Isolated Lymphoid Follicles After Intestinal Transplantation

Meier

Docena

Ramisch

et al. 2014

American Journal of Transplantation

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“…Histopathological assessments were performed with special concern to ACR in each graft. In analysis regarding to ACR, these rejection grades were evaluated based on our previously established grading schema in SBT (13,14). None of the chosen biopsies had morphological or clinical evidence of concomitant infection, posttransplant lymphoproliferative disease or other complications.…”

Section: Patients and Controlsmentioning

confidence: 99%

MicroRNA Signature of Intestinal Acute Cellular Rejection in Formalin-Fixed Paraffin-Embedded Mucosal Biopsies

Asaoka

Sotolongo

Island

et al. 2012

American Journal of Transplantation

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Despite continuous improvement of immunosuppression, small bowel transplantation (SBT) is plagued by a high incidence of acute cellular rejection (ACR) that is frequently intractable. Therefore, there is a need to uncover novel insights that will lead to strategies to achieve better control of ACR. We hypothesized that particular miRNAs provide critical regulation of the intragraft immune response. The aim of our study was to identify miRNAs involved in intestinal ACR. We examined 26 small intestinal mucosal biopsies (AR/NR group; 15/11) obtained from recipients after SBT or multivisceral transplantation. We investigated the expression of 384 mature human miRNAs and 280 mRNAs associated with immune, inflammation and apoptosis processes. We identified differentially expressed 28 miRNAs and 58 mRNAs that characterized intestinal ACR. We found a strong positive correlation between the intragraft expression levels of three miRNAs (miR-142-3p, miR-886-3p and miR-132) and 17 mRNAs including CTLA4 and GZMB. We visualized these miRNAs within cells expressing CD3 and CD14 proteins in explanted intestinal allografts with severe ACR. Our data suggested that miRNAs have a critical role in the activation of infiltrating cells during intestinal ACR. These differences in miRNA expression patterns can be used to identify novel biomarkers and therapeutic targets for immunosuppressive agents.

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“…The endoscopic findings of acute rejection include blunted and short villi, edematous and friable mucosa, superficial or deep ulcers, and diffuse mucosal exfoliation; these findings can be mimicked by infection. The histopathological features of acute cellular rejection include a mixed but mostly mononuclear infiltrate with activated lymphocytes, villus blunting and crypt epithelial cell apoptosis [45]. The rejection is graded as indeterminate, mild, moderate or severe depending upon the extent of the mucosal injury and the degree of inflammatory infiltrate and apoptosis.…”

Section: Graft Rejectionmentioning

confidence: 99%

Current Status of Intestinal Failure and Intestinal Transplantation

2012

Pediatr Gastroenterol Hepatol Nutr

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Histological criteria for the identification of acute cellular rejection in human small bowel allografts: results of the pathology workshop at the VIII International Small Bowel Transplant Symposium

Cited by 189 publications

References 5 publications

Immunological Status of Isolated Lymphoid Follicles After Intestinal Transplantation

Immunological Status of Isolated Lymphoid Follicles After Intestinal Transplantation

MicroRNA Signature of Intestinal Acute Cellular Rejection in Formalin-Fixed Paraffin-Embedded Mucosal Biopsies

Current Status of Intestinal Failure and Intestinal Transplantation

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