Histological characteristics of the gonads of pig fetuses and their relationship with fetal anatomical measurements

Pontelo, Thaís Preisser; Miranda, José Romilson Paes de; Félix, Matheus Augusto Rodrigues; Pereira, Bárbara Azevedo; Silva, William Eduardo da; Avelar, Gleide Fernandes de; Mariano, Flávia Cristina Martins Queiroz; Guimarães, Gregório Corrêa; Zangerônimo, Márcio Gilberto

doi:10.1016/j.rvsc.2017.11.005

Cited by 8 publications

(10 citation statements)

References 49 publications

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“…Other key developmental features, such as fusion of the palate also mark this transition, as well as gonadal differentiation. Gonadal differentiation has been studied in the pig and occurs at E (embryonic day) 50 in the pig (Pontelo et al, 2018). We therefore deduced that the end of the first trimester occurs at E50.…”

Section: The Mec and Lec Boundaries Can Be Delineated In The Prenatal Porcine Brainmentioning

confidence: 99%

Development of the Entorhinal Cortex Occurs via Parallel Lamination During Neurogenesis

et al. 2021

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The entorhinal cortex (EC) is the spatial processing center of the brain and structurally is an interface between the three layered paleocortex and six layered neocortex, known as the periarchicortex. Limited studies indicate peculiarities in the formation of the EC such as early emergence of cells in layers (L) II and late deposition of LIII, as well as divergence in the timing of maturation of cell types in the superficial layers. In this study, we examine developmental events in the entorhinal cortex using an understudied model in neuroanatomy and development, the pig and supplement the research with BrdU labeling in the developing mouse EC. We determine the pig serves as an excellent anatomical model for studying human neurogenesis, given its long gestational length, presence of a moderate sized outer subventricular zone and early cessation of neurogenesis during gestation. Immunohistochemistry identified prominent clusters of OLIG2+ oligoprogenitor-like cells in the superficial layers of the lateral EC (LEC) that are sparser in the medial EC (MEC). These are first detected in the subplate during the early second trimester. MRI analyses reveal an acceleration of EC growth at the end of the second trimester. BrdU labeling of the developing MEC, shows the deeper layers form first and prior to the superficial layers, but the LV/VI emerges in parallel and the LII/III emerges later, but also in parallel. We coin this lamination pattern parallel lamination. The early born Reln+ stellate cells in the superficial layers express the classic LV marker, Bcl11b (Ctip2) and arise from a common progenitor that forms the late deep layer LV neurons. In summary, we characterize the developing EC in a novel animal model and outline in detail the formation of the EC. We further provide insight into how the periarchicortex forms in the brain, which differs remarkably to the inside-out lamination of the neocortex.

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Section: The Mec and Lec Boundaries Can Be Delineated In The Prenatal Porcine Brainmentioning

confidence: 99%

Development of the Entorhinal Cortex Occurs via Parallel Lamination During Neurogenesis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Other key developmental features, such as fusion of the palate also mark this transition, as well as gonadal differentiation. Gonadal differentiation has been studied in the pig and occurs at E (embryonic day) 50 in the pig (Pontelo, Miranda et al 2018). We therefore deduced that the end of the first trimester occurs at E50.…”

Section: Subheading 1 the Mec And Lec Boundaries Can Be Delineated Imentioning

confidence: 99%

Development of the entorhinal cortex occurs via parallel lamination during neurogenesis

Liu

Bergmann

Mori

et al. 2021

Preprint

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The entorhinal cortex (EC) is the spatial processing center of the brain and structurally is an interface between the three layered paleocortex and six layered neocortex, known as the periarchicortex. Limited studies indicate peculiarities in the formation of the EC such as early emergence of cells in layers (L) II and late deposition of LIII, as well as divergence in the timing of maturation of cell types in the superficial layers. In this study, we examine developmental events in the entorhinal cortex using an understudied model in neuroanatomy and development, the pig and supplement the research with BrdU labeling in the developing mouse EC. We determine the pig serves as an excellent anatomical model for studying human neurogenesis, given its long gestational length, presence of a moderate sized outer subventricular zone and early cessation of neurogenesis during gestation. Immunohistochemistry identified prominent clusters of OLIG2+ oligoprogenitor-like cells in the superficial layers of the lateral EC (LEC) that are sparser in the medial EC (MEC). These are first detected in the subplate during the early second semester. MRI analyses reveal an acceleration of EC growth at the end of the second trimester. BrdU labeling of the developing MEC, shows the deeper layers form first and prior to the superficial layers, but the LV/VI emerges in parallel and the LII/III emerges later, but also in parallel. We coin this lamination pattern parallel lamination. The early-born Reln+ stellate cells in the superficial layers express the classic LV marker, Bcl11b (Ctip2) and arise from a common progenitor that forms the late deep layer LV neurons. In summary, we characterize the developing EC in a novel animal model and outline in detail the formation of the EC. We further provide insight into how the periarchicortex forms in the brain, which differs remarkably to the inside-out lamination of the neocortex.

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“…Considering the prevalence of small birthweight piglets, and the severity of this reproductive phenotype, it is important that there be improvement in understanding of the mechanisms regulating foetal ovarian development to improve reproductive efficiency. Whilst in previous studies the temporal changes in follicle types during gestation have been elucidated (Oxender et al, 1979;Pontelo et al, 2018), there is a limited understanding of the expression profiles of genes, as indicated by relative abundance of mRNA transcripts, associated with ovarian development and function.…”

Section: Introductionmentioning

confidence: 99%

Associations between foetal size and ovarian development in the pig

Stenhouse

Cortes‐Araya

Hogg

et al. 2020

Animal Reproduction Science

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It is estimated that intra-uterine growth restricted piglets represent 25% of the total number of piglets born. Growth restricted female piglets have impaired reproductive performance postnatally, however, when during gestation this phenotype arises is not known. With this study, the aim was to improve the understanding of foetal ovarian development in normal and small foetuses throughout gestation. Female Large White X Landrace foetuses were obtained at gestational day (GD) 45, 60 and 90 (n = 5-6 litters/GD). Histological analysis of GATA4 stained foetal ovaries at GD60 and 90 indicated there were fewer primary follicles (P ≤ 0.05) in the foetuses weighing the least compared to those with a weight similar to the mean for the litter (CTMLW) at GD90. Plasma oestradiol concentrations were less in the foetuses with lesser weights compared with greater weight foetuses at GD90 (P ≤ 0.05). The RNA was extracted from ovaries of the lesser weight and CTMLW foetuses at GD45, 60 and 90 and qPCR was performed to quantify relative abundance of 12 candidate mRNAs for which encoded proteins modulate ovarian function and development. Gestational changes in relative abundances of CD31, PTGFR, SPP1 and VEGFA mRNA transcripts were observed. Relative abundance of KI67 (P = 0.066) and P53 (P ≤ 0.05) was less in ovaries of the 3 lesser weight compared to CTMLW foetuses at GD60.

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Histological characteristics of the gonads of pig fetuses and their relationship with fetal anatomical measurements

Cited by 8 publications

References 49 publications

Development of the Entorhinal Cortex Occurs via Parallel Lamination During Neurogenesis

Development of the Entorhinal Cortex Occurs via Parallel Lamination During Neurogenesis

Development of the entorhinal cortex occurs via parallel lamination during neurogenesis

Associations between foetal size and ovarian development in the pig

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