Histological changes of liver tissue and serobiochemical relation in does with pregnancy ketosis

Abstract

ABSTRAKAzmi AFM, Ghani AAA, Saadan AS, Mokrish A, Lai KS, Zamri-Saad M, Zuki AB, Hassim HA. 2016. Perubahan histologi jaringan hati dan hubungan serum biokimia pada kambing yang mengalami kebuntingan ketosis. JITV 21(2): 96-100. DOI: http://dx.doi.org/10.14334/jitv.v21i2.1357Perubahan histologi hati kambing yang mengalami kebuntingan ketosis dikarakterisasi pada penelitian ini. Dua puluh kambing bunting pada hari ke-80 digunakan pada percobaan ini. Sepuluh kambing bunting diberi pakan rumput (Napier) dan konsentrat bersama air secara ad libitum dikategorikan sebagai kambing bunting yang sehat dan 10 kambing lainnya yang menunjukkan gejala-gejala klinik dan subklinik ketosis dikategorikan sebagai kambing bunting tidak sehat. Biopsi hati dilakukan saat gejala klinik muncul. Beta-Hydroxybutyrate (BHBA), asam lemak bebas, dan gula ditentukan dosisnya. Persiapan histologi menunjukkan persamaan insidensi dan intensitas steatosis hati ringan bersama vakuolasi seluler yang lebih rendah di dalam hepatosit kambing bunting yang sehat. Hampir semua kambing bunting yang menderita ketosis (n=8/10) mempunyai jumlah kadar tetesan lipid yang besar di dalam setiap hepatosit di semua asinus hati bersama kadar selular vakuolasi yang tinggi, serta berhubungan dengan peningkatan kadar BHBA dan asam lemak bebas saat terjadi penurunan kadar gula dalam darah. Histological changes of liver in does with pregnancy ketosis were characterized. Twenty pregnant does at day 80 of pregnancy were used for this experiment. A total of 10 does were fed by grass (Napier) and goat concentrate with water ad libitum. Those 10 goats considered as healthy pregnant goat, and another 10 goats showing clinical and subclinical signs of ketosis considered as unhealthy pregnant does. Liver biopsies were performed when clinical signs appeared. BetaHydroxybutyrate (BHBA), free fatty acid (FFA), and glucose were dosed. Histological preparation revealed similar incidence and intensity of mild liver steatosis with lower cellular vacuolation in hepatocyte presence in healthy late pregnant does. Almost all of the pregnant does with ketosis state (n=8/10) had large amount of small lipid droplets in almost every hepatocyte over the whole liver acinus with higher number of cellular vacuolation, and related with higher BHBA and FFA levels while low in glucose level.

Clinical Signs and Blood Variables of Pregnancy Toxemia Goats during Late Gestation and Postpartum

“…Diagnosis of clinical pregnancy toxemia is based on history, clinical signs of hepatic encephalopathy, and serum biochemical profiles (De Lima et al, 2012). A previous study showed that the detection of pregnancy toxemia could be determined by observing the changes of serum biochemical profiles in blood and clinical signs of pregnant goats (Azmi et al, 2016). On the other hand, Duffield et al (2009) concluded that subclinical pregnancy toxemia is a common metabolic disorder that affects goats in the transition period or in early lactation.…”