Histological changes in experimental periodontal disease in rats mono-infected with a gram-negative organism

Irving, J. T.; Newman, Michael G.; Socransky, S. S.; Heeley, J.D.

doi:10.1016/0003-9969(75)90013-8

Cited by 108 publications

(61 citation statements)

References 12 publications

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“…Indeed, a number of studies have shown that in gnotobiotic animals infected with a variety of Gram-posi tive bacteria, a voluminous bacterial mass sticks to the teeth in areas of periodontal breakdown. [40][41][42][43] However, recent observations of gnotobiotic animals monoinfected with Gram-negative bacteria 44 have shown that rapidly progressing periodontal disease may take place in the presence of comparatively few bacteria, which may only be detectable by cultural techniques and cannot be seen grossly or even microscopically. These observations suggest that Gram-negative bacteria may cause periodontal tissue destruction by different means than Gram-positive bacteria, and that adherence to the tooth or the presence of large numbers of cells may not be as important for periodontal disease as is commonly believed.…”

Section: Discussionmentioning

confidence: 99%

Structure of the Microbial Flora Associated with Periodontal Health and Disease in Man: A Light and Electron Microscopic Study

Listgarten

1976

Journal of Periodontology

498

304

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Teeth slated for extraction were evaluated with respect to their periodontal status and classified accordingly into five categories; namely normal, gingivitis, periodontitis, periodontosis and postperiodontosis. After processing, one approximal surface of each tooth was sampled at various levels in an apico-occlusal direction for light and electron microscopic study of the associated bacterial flora. In normal samples, the flora consisted of a relatively thin, adherent bacterial layer confined to the enamel surface. The cells were predominantly coccoid in shape with cell wall features compatible with those of Gram-positive organisms. Isolated filamentous or branching forms and some Gram-negative bacteria were noted on the surface of the more apical portion of the bacterial layer. No flagellated cells or spirochetes were present. Gingivitis samples yielded a relatively more voluminous and complex supragingival flora with relatively more filamentous bacteria and more cells with a Gram-negative cell wall ultrastructure. These samples also contained corncob formations on the surface of supragingival deposits, and flagellated cells with spirochetes within the predominantly Gram-negative flora of the sulcus bottom. Supragingival bacterial deposits of periodontitis samples were similar to those observed in gingivitis. The subgingival flora consisted of relatively fewer cells adherent to the root surface with a concomitant increase in the population of Gram-negative and flagellated cells, as well as spirochetes. The tissue side of the subgingival flora generally exhibited a distinctive concentration of "test-tube brush" formations, spirochetes of predominantly medium size, and assorted cell types peculiar to this region. A transitional flora generally separated the supra- from the subgingival microbial population. Periodontosis samples had a relatively sparse, predominantly Gram-negative flora. A unique electron-dense, lobulated cuticular deposit covered the majority of the samples studied. Postperiodontosis samples were much more similar in their microbial flora to the periodontitis group. The results suggest that (1) a certain microbial flora may be compatible with a state of periodontal health; (2) a different flora is associated with varying degrees of periodontal disease; (3) the structure and composition of the supragingival flora differs markedly from that of the subgingival flora; (4) with the exception of periodontis, the alterations of the microbial flora as periodontal disease increases inseverity parallel the changes described previously in the microbial population collected on artificial crowns during experimentally induced gingivitis. The use of the expressions "microbial flora" or "microbial population" is considered preferable to the terms "plaque", "materia alba", or "debris" in reference to the microbiota of the gingival sulcus region.

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Section: Discussionmentioning

confidence: 99%

Structure of the Microbial Flora Associated with Periodontal Health and Disease in Man: A Light and Electron Microscopic Study

Listgarten

1976

Journal of Periodontology

498

304

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“…2c). It should be realized that this strain has been assigned a particularly high periodontopathic potential in humans and animals (11,33). Previously, we have found that strain FDC Y4 differs from most other strains of A .…”

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confidence: 89%

Notes: Differentiation Between Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by High-Resolution, Two-Dimensional Protein Electrophoresis

Jellum

Tingelstad

Olsen

1984

International Journal of Systematic Bacteriology

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The cellular proteins of eight strains of Actinobacillus actinomycetemcomitans and seven strains of Haemophilus aphrophilus were analyzed by two-dimensional electrophoresis and silver staining. The electrophoretic patterns of A . actinomycetemcomitans and H . aphrophilus were so different that distinction between these closely related bacteria could easily be made. There were also minor quantitative and qualitative differences in the protein patterns of strains within both species, enabling, particularly, A . actinomycetemcomitans strains FDC 2097 and FDC Y4 and H . aphrophilus strains ATCC 19415, FDC 626, and FDC 621 to be singled out.

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“…Dental researchers regard the discoveries of mutans streptococci (1), in the middle of the 20th century, and the specific periodontal pathogens Aggregatibacter actinomycetemcomitans (2), Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia (3), thereafter, as elements of the classical infectious etiology proposed by Koch. However, mutans streptococci are not frequently detected in dentin lesion caries.…”

Section: Introductionmentioning

confidence: 99%

The oral microbiome and human health

Yamashita

Takeshita

2017

Journal of Oral Science

244

152

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Abstract:In this brief review, we discuss our previous research on the relationship between the bacterial composition of salivary microbiota and periodontal disease. Analysis using a terminal restriction fragment length polymorphism method and an international comparison suggest that the predominance of the genera Prevotella and Veillonella in the salivary microbiota is attributable to periodontal disease conditions, and that the predominance of the genus Neisseria indicates healthy periodontal conditions. Furthermore, we recently used next-generation sequencing technology to perform a detailed largescale analysis of the salivary microbiota. An important finding of that study was that high bacterial richness in the salivary microbiota was significantly associated with poor oral health, as indicated by decayed teeth, periodontitis, and poor oral hygiene. Another important result was that relative abundance of predominant bacteria in saliva was significantly associated with oral health-related conditions. Of the two different cohabiting groups of bacteria found in the salivary microbiota, a greater relative abundance of group I bacteria, which include Prevotella and Veillonella species, was associated with poor oral health, high body mass index, and old age. These findings suggest that the salivary microbiota reflects oral and systemic conditions.

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Histological changes in experimental periodontal disease in rats mono-infected with a gram-negative organism

Cited by 108 publications

References 12 publications

Structure of the Microbial Flora Associated with Periodontal Health and Disease in Man: A Light and Electron Microscopic Study

Structure of the Microbial Flora Associated with Periodontal Health and Disease in Man: A Light and Electron Microscopic Study

Notes: Differentiation Between Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by High-Resolution, Two-Dimensional Protein Electrophoresis

The oral microbiome and human health

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