Histological appearances of intracranial biopsies obtained using the Cavitron ultrasonic surgical aspirator.

Blackie, R. A. S.; Gordon, Adam

doi:10.1136/jcp.37.10.1101

Cited by 23 publications

(8 citation statements)

References 5 publications

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“…16,17 Typically, aerosolized tum our cells are dispersed within an environment, which has been supporting their grow th. W hile aerosolization may result in morphologic changes, which affect their long-term viability, 18,19 our observations as well as previous reports point to potential viability of these cells. 6,7 …”

Section: Discussionsupporting

confidence: 82%

Tumour cell dispersion by the ultrasonic aspirator during brain tumour resection

Preston

Masciopinto

Salamat

et al. 1999

British Journal of Neurosurgery

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Ultrasonic aspirators are commonly used to resect brain tumours because they allow safe, rapid and accurate removal of diseased tissue. Since ultrasonic aspirators generate a spray of aerosolized irrigating fluid around the instrument tip, we questioned whether this spray might contain viable tumours cells that could contribute to intraoperative spread of tumour fragments. To test this hypothesis, we collected the spray produced during the resection of nine brain tumours with an ultrasonic aspirator and semi-quantitatively analysed it for tumour presence. The aerosolized irrigation fluid was found to contain intact tumour cells or clumps of tumour cells in all nine instances, and there was a trend of increasing tumour cell dispersion with increasing ultrasonic aspiration times. Further examination is required to determine if this intraoperative dispersion of apparently viable tumour fragments contributes to local neoplasm recurrence.

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Section: Discussionsupporting

confidence: 82%

Tumour cell dispersion by the ultrasonic aspirator during brain tumour resection

Preston

Masciopinto

Salamat

et al. 1999

British Journal of Neurosurgery

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“…As one of the first research groups, Malhotra et al [1] investigated whether ultrasonically aspirated tissue can be used for histopathological studies, and they found positive results for all resected tumors. Similar observations have been made by Blackie and Gordon [2], who investigated tumor tissue fragments from 17 resections and were able to give the correct diagnosis for all aspirated samples. Furthermore, Oakes et al [3] could establish cell cultures from CUSA material of patients with medulloblastoma.…”

Section: Introductionsupporting

confidence: 66%

“…CUSA EXcel) might consistently have a greater amount of vital tumor cells than tissue obtained by standard biopsy methods, especially glioma tissue; furthermore, it is much more easily retrieved from the suction trap than from a sterile disposable sack within the system. Thus, the utilization of material from the suction trap may facilitate pathological interpretation [2] by providing a more representative sample of tumor histology as well as an adequate, sterile and convenient source of tumor material for further cellular or molecular studies [7]. …”

Section: Discussionmentioning

confidence: 99%

Glioma Tissue Obtained by Modern Ultrasonic Aspiration with a Simple Sterile Suction Trap for Primary Cell Culture and Pathological Evaluation

Schroeteler¹,

Reeker²,

Molina³

et al. 2014

Eur Surg Res

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Objectives: Ultrasonic aspiration is widely used in the resection of brain tumors. Nevertheless, tumor tissue fragments obtained by ultrasonic aspiration are usually discarded. In this study, we demonstrate that these fragments are possible sources of material for histopathological study and tissue culture and compare their microscopic features and viability in tissue culture of cavitron ultrasonic surgical aspirator tissue fragments. Methods: Brain tumor tissue collected by ultrasonic aspiration (CUSA EXcel®; Integra Radionics Inc.) in a simple sterile suction trap during resection was processed for primary cell culture. Cell viability and immunohistological markers were measured by the WST-1 test, microscopy and immunofluorescent evaluation. Results: Six gliomas are presented to demonstrate that these tissue fragments show good preservation of histological detail and tissue viability in culture. Conclusion: Utilization of this material may facilitate pathological interpretation by providing a more representative sample of tumor histology as well as an adequate and sterile biosource of material for tissue culture studies.

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“…There have been three previous reports describing the histopathology of CNS tumors removed with the CUSA. [5][6][7] We recently evaluated the cytomorphologic appearance of 22 CNS tumors obtained by the CUSA and analyzed which types of the neoplasms showed the best preser~ation.~ The study reported in the present article investigated the immunocytochemical staining patterns of the material obtained with the CUSA, which in some cases was the only biopsy material available. Knowledge of the reliability of immunocytochemical reactions on CUSA material from a given tumor is of considerable importance to the surgical pathologist and cytologist, so presented herein are the results of immunocytochemical reactions for 11 cases of meningioma, three cases of glioblastoma multiforme, one astrocytoma, and two schwannomas.…”

mentioning

confidence: 99%

Immunocytochemical evaluation of central nervous system tumors obtained by the cavitron ultrasonic surgical aspirator

Finley¹,

Silverman²,

Dickens³

1990

Diagnostic Cytopathology

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The Cavitron ultrasonic surgical aspirator (CUSA) is a dissecting system that allows quick and effective removal of CNS tumors without traction or excessive manipulation of normal tissue. In this article, the immunoperoxidase staining patterns of cytology specimens obtained with the CUSA are compared with those from their corresponding resected surgical specimens employing a battery of monoclonal and polyclonal antibodies. Eleven cases of meningioma, three cases of glioblastoma multiforme, one astrocytoma, and two schwannomas were evaluated. In both CUSA cytologic biopsies and surgical biopsies, all the meningiomas showed strong staining for vimentin and epithelial membrane antigen, while two showed focal staining for cytokeratins. The glioblastoma multiforme and astrocytoma cases showed positivity for vimentin, S-100 protein, and glial fibrillary acidic protein, while the schwannomas stained positively for vimentin and S-100 protein. With only rare exceptions, the immunocytochemistry of the CUSA and surgical specimens correlated well in all of these cases in terms of strength of reaction and localization. There were no false-positive staining reactions in the CUSA material. This study suggests that reliable morphologic and immunoperoxidase studies can be performed on cytologic material obtained by the CUSA, which could aid in making an accurate and specific diagnosis of a variety of CNS tumors.

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Histological appearances of intracranial biopsies obtained using the Cavitron ultrasonic surgical aspirator.

Cited by 23 publications

References 5 publications

Tumour cell dispersion by the ultrasonic aspirator during brain tumour resection

Tumour cell dispersion by the ultrasonic aspirator during brain tumour resection

Glioma Tissue Obtained by Modern Ultrasonic Aspiration with a Simple Sterile Suction Trap for Primary Cell Culture and Pathological Evaluation

Immunocytochemical evaluation of central nervous system tumors obtained by the cavitron ultrasonic surgical aspirator

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