Histological and ultrastructural evaluation of human decellularized matrix as a hernia repair device

Ghetti, Martina; Papa, Valentina; Deluca, Giovanni; Purpura, Valeria; Ruscelli, Paolo; Melandri, Davide; Capirossi, Daniela; Nigrisoli, Evandro; Minghetti, Paola; Bondioli, Elena; Cenacchi, Giovanna

doi:10.1080/01913123.2017.1365788

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…Our group, in particular, has achieved excellent results through using decellularized dermal matrices in the treatment of several clinical conditions, such as abdominal wall defect, breast, and pelvic reconstruction, as well as rotator cuff repair. 67–72 While dermal matrices are a very popular naturally derived biomaterial, few groups have shown interest in developing scaffolds by decellularizing foreskin tissue in a comparable manner to this work. One group had promising results using their foreskin-derived matrix in urethral tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

The development of a decellularized extracellular matrix–based biomaterial scaffold derived from human foreskin for the purpose of foreskin reconstruction in circumcised males

et al. 2018

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The circumcision of males is emphatically linked to numerous sexual dysfunctions. Many of the purported benefits do not hold up to the scrutiny of extensive literature surveys. Involuntary circumcision, particularly when not medically warranted, is also associated with many psychological and emotional traumas. Current methods to reconstruct the ablated tissue have significant drawbacks and produce a simple substitute that merely imitates the natural foreskin. Extracellular matrix–based scaffolds have been shown to be highly effective in the repair and regeneration of soft tissues; however, due to the unique nature of the foreskin tissue, commercially available biomaterial scaffolds would yield poor results. Therefore, this study discusses the development and evaluation of a tissue engineering scaffold derived from decellularized human foreskin extracellular matrix for foreskin reconstruction. A chemicophysical decellularization method was applied to human foreskin samples, sourced from consenting adult donors. The resulting foreskin dermal matrices were analyzed for their suitability for tissue engineering purposes, by biological, histological, and mechanical assessment; fresh frozen foreskin was used as a negative control. Sterility of samples at all stages was ensured by microbiological analysis. MTT assay was used to evaluate the absence of viable cells, and histological analysis was used to confirm the maintenance of the extracellular matrix structure and presence/integrity of collagen fibers. Bioactivity was determined by submitting tissue extracts to enzyme-linked immunosorbent assay and quantifying basic fibroblast growth factor content. Mechanical properties of the samples were determined using tensile stress tests. Results found foreskin dermal matrices were devoid of viable cells (p < 0.0001) and the matrix of foreskin dermal matrices was maintained. Basic fibroblast growth factor content doubled within after decellularization (p < 0.0001). Tensile stress tests found no statistically significant differences in the mechanical properties (p < 0.05). These results indicate that the derived foreskin dermal matrix may be suitable in a regenerative approach in the reconstruction of the human foreskin.

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Section: Discussionmentioning

confidence: 99%

The development of a decellularized extracellular matrix–based biomaterial scaffold derived from human foreskin for the purpose of foreskin reconstruction in circumcised males

et al. 2018

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“…Ghetti et al. evaluated the post-operative morphological response of human decellularized matrices in hernia patients after one-year post-transplantation [ 113 ]. The observed results were that the implant site was characterized by regenerative cellular recruitment, neo-vascularization, reduced inflammatory response, and a well-organized collagen matrix.…”

Section: Commercial Meshes Used In Clinics For Hernia Repairmentioning

confidence: 99%

Abdominal wall hernia repair: from prosthetic meshes to smart materials

Saiding,

Chen,

Wang

et al. 2023

Materials Today Bio

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“…dECM biomaterial products have already successfully demonstrated their bioactivity as providers of a rich and stimuli-charged microenvironment for cells, being able to influence cellular behaviour and even modulate the immune system's response, thus representing optimal candidates from a regenerative point of view [3,4]. Because of this, effective results have been reported using dECM biomaterials for wound 2 of 17 healing [5], nervous system repair [6], liver fibrosis treatment [7], myocardial infarction treatments [8], corneal repair [9], cartilage repair [10], tendon repair [11], hernias [12], and other applications [1,13]. To date, the clear mechanism through which dECM biomaterials are able to guide cellular processes and the immune system to promote tissue regeneration, especially in pathological cases, is not completely clear.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of Matrix-Bound Nanovesicles (MBVs) Isolated from Decellularised Bovine Pericardium: New Frontiers in Regenerative Medicine

Di Francesco,

Di Varsavia,

Casarella

et al. 2024

IJMS

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Matrix-bound nanovesicles (MBVs) are a recently discovered type of extracellular vesicles (EVs), and they are characterised by a strong adhesion to extracellular matrix structural proteins (ECM) and ECM-derived biomaterials. MBVs contain a highly bioactive and tissue-specific cargo that recapitulates the biological activity of the source ECM. The rich content of MBVs has shown to be capable of potent cell signalling and of modulating the immune system, thus the raising interest for their application in regenerative medicine. Given the tissue-specificity and the youthfulness of research on MBVs, until now they have only been isolated from a few ECM sources. Therefore, the objective of this research was to isolate and identify the presence of MBVs in decellularised bovine pericardium ECM and to characterise their protein content, which is expected to play a major role in their biological potential. The results showed that nanovesicles, corresponding to the definition of recently described MBVs, could be isolated from decellularised bovine pericardium ECM. Moreover, these MBVs were composed of numerous proteins and cytokines, thus preserving a highly potential biological effect. Overall, this research shows that bovine pericardium MBVs show a rich and tissue-specific biological potential.

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Histological and ultrastructural evaluation of human decellularized matrix as a hernia repair device

Cited by 5 publications

References 21 publications

The development of a decellularized extracellular matrix–based biomaterial scaffold derived from human foreskin for the purpose of foreskin reconstruction in circumcised males

The development of a decellularized extracellular matrix–based biomaterial scaffold derived from human foreskin for the purpose of foreskin reconstruction in circumcised males

Abdominal wall hernia repair: from prosthetic meshes to smart materials

Characterisation of Matrix-Bound Nanovesicles (MBVs) Isolated from Decellularised Bovine Pericardium: New Frontiers in Regenerative Medicine

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