Histological and quantitative analyzes of the stromal and acinar components of normal human prostate zones

Alves, Edilaine F.; Ribeiro, Bruno; Costa, Waldemar S.; Gallo, C.; Sampaio, Francisco J.B.

doi:10.1002/pros.23472

Cited by 10 publications

(6 citation statements)

References 22 publications

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“…Many studies have attempted to determine the morphological and molecular differences between TZ and PZ. 7 , 8 However, efforts to investigate these variances have primarily focused on anatomical zones rather than cellular composition and functional heterogeneity.…”

Section: Introductionmentioning

confidence: 99%

“…Although this clinical phenomenon has long been recognised, the underlying reasons and mechanisms have not yet been elucidated. Many studies have attempted to determine the morphological and molecular differences between TZ and PZ 7,8 . However, efforts to investigate these variances have primarily focused on anatomical zones rather than cellular composition and functional heterogeneity.…”

Section: Introductionmentioning

confidence: 99%

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Single‐cell RNA‐sequencing technology demonstrates the heterogeneity between aged prostate peripheral and transitional zone

Yan

Wang

Xia

et al. 2022

Clinical & Translational Med

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Background Identifying cellular and functional heterogeneity within aged prostate is critical for understanding the spatial distribution of prostate diseases. Methods Aged human prostate peripheral zone (PZ) and transitional zone (TZ) tissues were used for single‐cell RNA‐sequencing. Results were validated by immunofluorescence staining. Results We found that club/hillock epithelial cells, compared with other epithelial cells, had significantly higher NOTCH signaling activity and expressed higher levels of neuro‐stems but lower androgen‐related genes. These cells were primarily found in the TZ and provided a stem‐like niche around the proximal prostate ducts. Significant heterogeneity was observed in the aged luminal population. A novel TFF3+ luminal subtype with elevated MYC and E2F pathway activities was observed, primarily in the PZ. Further analysis revealed that epithelial cells in the TZ had higher levels of stem‐ and inflammation‐related pathway activities but lower androgen/lineage‐related pathway activities than those in the PZ. Notably, the activation of MYC, E2F and DNA repair pathways significantly increased in PZ luminal cells. In the immune landscape, we found that the immune microenvironment in the TZ is more complex and disordered with more infiltration of NK and Treg cells. CD8 T cell and macrophage in the TZ exhibit both inflammation activation and suppression phenotypes. In the stroma, the TZ had a higher fibroblast density, and fibroblasts in the TZ exhibited stronger transcriptome activity in immunity and proliferation. Ligand–receptor interaction analysis revealed that fibroblasts could contribute to a NOTCH signaling niche for club/hillock cells in the TZ and balance the prostate immune microenvironment. The activation of stem properties, inflammatory infiltration and loss of androgen/lineage activity are prominent features distinguishing the TZ from PZ. Conclusions Our study explains the heterogeneity between the TZ and PZ of aged prostate, which may help understand the spatial distribution of prostate diseases and establish a foundation for novel target discovery.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Single‐cell RNA‐sequencing technology demonstrates the heterogeneity between aged prostate peripheral and transitional zone

Yan

Wang

Xia

et al. 2022

Clinical & Translational Med

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“…However, these features might not be exclusively present in each zone and histological assessment needs to be done based on a tissue section rather than just based on cellular appearance alone. Since McNeal's work, further work has been conducted into classifying differences of histological features between zones such as epithelial (such as the height of epithelial cells and nerve density, higher in the nonmalignant PZ than the TZ) and stromal compartments (for example, the density of connective tissue is higher in TZ non-malignant tissue than the PZ), in keeping with previous findings by McNeal [62][63][64][65] .…”

Section: Further Work By Mcneal and Colleaguesmentioning

confidence: 55%

Prostate zones and cancer: lost in transition?

et al. 2021

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Localized prostate cancer shows great clinical, genetic and environmental heterogeneity; however, prostate cancer treatment is currently guided solely by clinical staging, serum PSA levels and histology. Increasingly, the role of differential genomics, multifocality and spatial distribution in tumorigenesis are being considered to further personalize treatment. The human prostate is divided into three zones based on its histological features: the peripheral zone (PZ), the transition zone (TZ) and the central zone (CZ). Each zone has variable prostate cancer incidence, prognosis and outcomes, with TZ prostate tumours having better clinical outcomes than PZ and CZ tumours. Molecular and cell biological studies can improve understanding of the unique molecular, genomic and zonal cell type features that underly the differences in tumour progression and aggression between the zones. The unique biology of each zonal tumour type could help guide individualized treatment and patient risk stratification.

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“…The amounts of detrusor muscle collagen and total collagen were determined using the point counting technique with an objective of 100x and 20x respectively and the same software. For all analyses, 5 fields per animal were considered (13, 14).…”

Section: Methodsmentioning

confidence: 99%

Effect of a high-fat diet on the rat bladder wall and bioactive action of Brazil nut oil

et al. 2019

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High-fat diet-induced obesity is associated with metabolic disorders. The Brazil nut has bioactive substances and has been used to control the damage caused by obesity in several organs. The work intended to show the damage caused by high-fat diet in the bladder wall and if the Brazil nut oil added to the diet could ameliorate or reverse this effect. Sixty-day-old rats were divided into two groups: C (control, n = 30) and HF (high-fat, n = 30) diets. At 90 days, 10 animals of each group were sacrificed. The others were divided into 4 groups: C and HF (animals that maintained their previous diet, n = 10 for each group) and C / Bno and HF / Bno (animals whose control or high-fat diet was supplemented by Brazil nut oil, n = 10 for each group). Sacrifice occurred at 120 days, and the bladders were removed and analyzed. Epithelial height was increased in the HF compared to the C group. In contrast, the C / Bno had a lower epithelial height compared to the others. The percentage of collagen between the detrusor muscle fibers was significantly greater in C / Bno, HF and HF / Bno than in control group. The HF had a larger muscle fiber diameter than the C group, while the C / Bno presented lower values than the HF and HF / Bno groups. HF diets induced bladder wall damage. These changes in the rat's bladder wall were partially reversed by the Bno.

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Histological and quantitative analyzes of the stromal and acinar components of normal human prostate zones

Abstract: Our objective, quantitative data regarding the various elements that constitute the normal prostate stroma allowed us to reveal differences among prostate zones. This study established patterns for normal parameters and may be used for posterior comparisons in histopathological analysis.

Cited by 10 publications

References 22 publications

Single‐cell RNA‐sequencing technology demonstrates the heterogeneity between aged prostate peripheral and transitional zone

Single‐cell RNA‐sequencing technology demonstrates the heterogeneity between aged prostate peripheral and transitional zone

Prostate zones and cancer: lost in transition?

Effect of a high-fat diet on the rat bladder wall and bioactive action of Brazil nut oil

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