Histological and immunohistochemical study of the effect of tramadol on the seminiferous tubules of adult albino rat and the effect of its withdrawal

Elnaga, Amira Ali Abou; Kassab, Amira Adly; Soliman, Gehan M.; Shal, Awatif Omar El

doi:10.4103/tmj.tmj_85_17

Cited by 6 publications

(6 citation statements)

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“…They also observed vacuolated homogenous acidophilic material of the interstitium, Pyknotic nuclei, binucleated cells, vacuolated spermatogonia, and spermatocytes, as well as deeply stained nuclei of Leydig cells. Ghoneim et al [51] revealed that TAM treatment for 4 weeks showed distinct histological changes. The seminiferous tubules existed in abnormal shape, broadly isolated from each other, and some tubules had ruptured without spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Chemotherapeutic Potential of Epimedium brevicornum Extract: The cGMP-Specific PDE5 Inhibitor as Anti-Infertility Agent Following Long-Term Administration of Tramadol in Male Rats

et al. 2020

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Epimedium brevicornum Maxim (EbM) is a well-known Chinese herb that has been widely used for the treatment of several diseases. The main purpose of this study is to examine the role of Epimedium brevicornum extract in certain andrological parameters in rats as a natural modulator for adverse viewpoints associated with chronic administration of tramadol (TAM). Fifty rats were categorized into five groups. Untreated rats were known as Group I, whereas rats in Groups II and III were administered 2.43 g/kg/day of E. brevicornum extract and 50 mg/kg/day of TAM for 130 consecutive days, respectively. Both of Groups IV and V were administered TAM for 65 successive days, followed by concomitant use of both drugs for another 65 days, with the E. brevicornum extract at doses of 0.81 and 2.43 g/kg/day, respectively. TAM showed an injurious effect on sperm attributes, serum hormones, tissue malondialdehyde, superoxide dismutase, and nitric oxide. Elevation of the apoptotic marker Bax and a reduction of Bcl2 were recorded. Histopathological abnormalities have been reported in rat testicles. Rats treated with E. brevicornum extract with TAM showed an improvement in all the parameters tested. It could be presumed that E. brevicornum extract plus TAM exhibits a promising effect on the enhancement of male anti-infertility effects.

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Section: Discussionmentioning

confidence: 99%

Chemotherapeutic Potential of Epimedium brevicornum Extract: The cGMP-Specific PDE5 Inhibitor as Anti-Infertility Agent Following Long-Term Administration of Tramadol in Male Rats

et al. 2020

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“…The present study documented testicular degenerative changes such as presence of large number of vacuoles in germinal epithelium, giant cells and lumen devoid of spermatozoa. The presence of giant cells and vacuoles within the seminiferous epithelium are associated with dilated endoplasmic reticulum, mitochondria and perinuclear space that represents impaired spermatogenesis and phagocytosis of defective germ cells (Elnaga et al, 2018, Gupta et al, 1998Ray et al, 1987;Barlow and Sullivan 1982;Working et al;1985;Datta and Dikshith 1976). Earlier report suggests that quinalphos exposure leads to marked decline in androgen level (Ray et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Effect of Quinalphos on Testes of Long Day Seasonal Breeder Golden Hamster, Mesocricetus auratus

Kumar¹,

Haldar²,

Verma³

2020

JSR

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The Environmental toxicants are emerging factor for male infertility. Thus, the aim of the present study was to investigate the effect of quinalphos on testes of long day seasonal breeder golden hamster, Mesocricetus auratus. Hamsters were randomly divided into two groups (n=5): group I-control and group II-quinalphos treated (250µg/kg BW/day, injected intraperitoneally for 14 days). The testicular histoarchitecture, sperm count, sperm viability, antioxidant enzyme activity and lipid peroxidation level of testes were evaluated. Quinalphos treatment altered testicular histoarchitecture and led to reproductive dysfunction as evident by reduced sperm count and sperm viability. Further, quinalphos treated hamsters showed marked decrease in testicular antioxidant enzyme (superoxide dismutase, SOD and catalase, CAT) activities with an increase in lipid peroxidation (MDA) level. In conclusion, our findings clearly suggest that quinalphos exposure induces testicular oxidative stress having negative impact on male reproductive function.

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“…Many types of research were performed to detect the biochemical and histopathological changes due to long-term abuse the tramadol on the liver, kidney, and brain, also some studies dealt with abnormal histological changes in the testis 10,11 .…”

Section: Introductionmentioning

confidence: 99%

“…13 The large effect of chronic use of tramadol in many body organs like thyroid, liver and kidney have been evaluated and reported by many researchers and scanty data dealt with the effects use of tramadol on the brain, therefore the objective of this study was designed to evaluate the toxic impact of the tramadol on the tissues of the brain in the male rats. 14,15…”

Section: Introductionmentioning

confidence: 99%

Histopathological Changes in Brain Tissues Associated with Oral Administration of Tramadol in Male Rats

Mraisel,

Ibrahim,

Dawood

2021

Indian Journal of Forensic Medicine & Toxicology

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Background: Repeated and long treatment with tramadol that could cause toxic metabolites by accumulation in the body and with high level of risk of pharmokinetic interaction alongside the decreasing of tramadol, therefore this study was carried out to investigate the toxic impact of the tramadol on the tissues of the brain in the male rats. Method: This experiment was accomplished and processed at environmental toxicology laboratory, environmental studies department, institute of graduate studies and research, university of Alexandria, Alexandria p) ovince, Egypt. a group of rats (32, male) from Albano waster subspecies and they weigh from 200-250g, all belonging to the animal house of the faculty of medicine, university of Alexandria and grouping in to four groups (8 rats for each group in each cage). In regards to the control group, they were given basal food and tap water through-out 10 days by gastric tube, day by day. Group three the rats were fed with basal diet and given Tramadol HCL orally in dose 45mg/ kg .B.W dissolved in (5ml) normal saline (0.9%) by gastric tube, daily for Twenty days. Group for the rats were fed with basal diet and given Tramadol HCL orally in dose 45mg/ kg .B.W dissolved in (5ml) normal saline (0.9%) by gastric tube, daily for Thirty days. When the experimental period reached its end, kidney tissues of each rat were instantly removed and after that weighed then put into 10 percent of neutral buffer formalin to be considered as a fixative solution.The Results: The results showed significantly decrease in the heaviness of the brain in the groups of the rats that given the Tramadol HCL in dose 45mg/ kg .B.W with increasing the time of administration as compared to the control group. Histopathological changes were observed in rats brain tissues section the rats that given Tramadol HCL orally in dose 45mg/ kg .B.W dissolved in (5ml) normal saline for ten days revealed mild degree of tissue injury in the cerebral cortex, with few vacuolar degeneration and dilatation of blood vessels, and the tissue sections of group two after ten days revealed mild degree of tissue injury in the cerebral cortex, with few vacuolar degeneration and dilatation of blood vessels, while the three group Observed increase in the vacuolar degeneration, with neural atrophy and degeneration of neurons with reduction the neural process and pyknosis of the nuclei dilatation of blood vessels after twenty days of tramadol administration. The tissue Sections the obtained from the group four after thirty days revealed increase in the vacuolar degeneration, with more atrophy of the neural cells and complete reduction the neural process and pyknosis of the nucleus in the injured neural cells and glyosis.The Conclusion of this study there are harmful toxic effects when administrated the tramadol for long period on the brain tissues, therefore abuse of tramadol should be avoided except with medical prescription owing to its toxic effects.

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Histological and immunohistochemical study of the effect of tramadol on the seminiferous tubules of adult albino rat and the effect of its withdrawal

Cited by 6 publications

References 2 publications

Chemotherapeutic Potential of Epimedium brevicornum Extract: The cGMP-Specific PDE5 Inhibitor as Anti-Infertility Agent Following Long-Term Administration of Tramadol in Male Rats

Chemotherapeutic Potential of Epimedium brevicornum Extract: The cGMP-Specific PDE5 Inhibitor as Anti-Infertility Agent Following Long-Term Administration of Tramadol in Male Rats

Effect of Quinalphos on Testes of Long Day Seasonal Breeder Golden Hamster, Mesocricetus auratus

Histopathological Changes in Brain Tissues Associated with Oral Administration of Tramadol in Male Rats

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