Histological and Immunohistochemical Revision of Hepatocellular Adenomas: A Learning Experience

Fonseca, Sandhya; Hoton, Delphine; Dardenne, Sabrina; Annet, Laurence; Hubert, Catherine; Godecharles, S.; Jouret‐Mourin, Anne; Reding, Raymond; Otte, Jean Bernard; Rahier, Jacques; Gigot, Jean‐François; Sempoux, Christine

doi:10.1155/2013/398308

Cited by 25 publications

(21 citation statements)

References 28 publications

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“…Recently, Fonseca et al . reported one case of β‐catenin‐activated/HNF1α‐inactivated HCA . Castain et al .…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Fonseca et al reported one case of β-catenin-activated/HNF1αinactivated HCA. 28 Castain et al showed the coexistence of inflammatory HCA with H-HCA in nine cases. 29 In the present study, we carefully differentiated these HCA from HCC using histological and immunohistochemical methods because, in our additional study, we showed the loss of LFABP expression in approximately 10% of HCC, which was closely associated with β-catenin activation (unpubl.…”

Section: W E Distinguished Four Subtypes and Twomentioning

confidence: 99%

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Preserved or enhanced OATP1B3 expression in hepatocellular adenoma subtypes with nuclear accumulation of β‐catenin

Fukusato

Soejima

Kondo

et al. 2014

Hepatology Research

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Aim Recent studies have indicated that hepatocellular adenoma (HCA) is a heterogenous group of benign tumors with various genetic and clinicopathological characteristics. We delineated the clinicopathological characteristics of HCA subtypes and evaluated the expression of transporter protein OATP1B3 in HCA. Methods HCA in 34 Japanese patients were investigated immunohistochemically and classified into four subtypes (HNF1α‐inactivated type, H‐HCA; β‐catenin‐activated type, b‐HCA; inflammatory type, I‐HCA; unclassified type, u‐HCA). Immunostaining of OATP1B3 protein in HCA tissue sections was performed to determine the association between OATP1B3 expression and HCA subtypes. Results HCA was categorized into the following four subtypes and two combined subtypes: 10 H‐HCA (29%), 10 I‐HCA (29%), seven b‐HCA (21%), two b‐HCA/H‐HCA (6%), two b‐HCA/I‐HCA (6%) and three u‐HCA (9%). The male‐to‐female ratio was 18:16. Oral contraceptive use was rare but seven HCA were found in patients with glycogen storage disease, congenital absence of the portal vein and idiopathic portal hypertension. OATP1B3 expression was decreased in 24 HCA but was preserved or increased in 10 HCA. All nine HCA with nuclear staining for β‐catenin showed preserved or enhanced OATP1B3 expression, indicating a significant association between nuclear β‐catenin accumulation and OATP1B3 expression in HCA. Conclusion HCA subtype classification was validated in 91% of our Japanese subjects although their clinical backgrounds including rare contraceptive use were different from European subjects. A close association between preserved or enhanced OATP1B3 expression and b‐HCA subtype indicated important modalities for clinical decisions in the treatment and follow up of patients with HCA.

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“…Recently, Fonseca et al . reported one case of β‐catenin‐activated/HNF1α‐inactivated HCA . Castain et al .…”

Section: Discussionmentioning

confidence: 99%

Section: W E Distinguished Four Subtypes and Twomentioning

confidence: 99%

Preserved or enhanced OATP1B3 expression in hepatocellular adenoma subtypes with nuclear accumulation of β‐catenin

Fukusato

Soejima

Kondo

et al. 2014

Hepatology Research

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“…One phenotype consisted of atypical hepatocytes with microvesicular lipid droplets. Immunohistochemically, these cells did not express liver fatty acid‐binding protein and glutamine synthetase (GS), both of which are found in HNF‐1α‐inactivated hepatocellular adenoma . The second phenotype of liver tumors consisted of atypical hepatocytes with diffuse expression of GS, glypican‐3, alpha‐fetoprotein and nuclear expression of beta‐catenin, thus resembling HCC in humans (Fig.…”

Section: Ms–nash‐hcc Model Micementioning

confidence: 99%

Animal models for analyzing metabolic syndrome‐associated liver diseases

Tsuneyama

Nishitsuji

Matsumoto

et al. 2017

Pathology International

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Metabolic syndrome (MS) is a worldwide healthcare issue and a dominant risk factor for the development of incurable diseases affecting the entire body. The hepatic manifestations of MS include nonalcoholic fatty liver disease (NAFLD) and its progressive variant, nonalcoholic steatohepatitis (NASH). NASH is known to progress to liver cirrhosis and hepatocellular carcinoma (HCC). Excellent animal models for determining the mechanism of pathogenesis and establishing therapeutic treatment of NASH/HCC are strongly required worldwide. We recently reported that two previously established mouse models of obesity and diabetes mellitus, namely, Tsumura-Suzuki Obese Diabetes (TSOD) mice and MSG mice, developed MS-associated NASH and that their clinical course and pathological characteristics closely mimicked those of human MS-NASH patients. Interestingly, most of the mice developed HCC with advancing age, and the pathological and functional characteristics of this condition were identical to those of human HCC. We further established a novel mouse model of HCC based on type 1 diabetes (DIAR-nSTZ mice) and reported its histopathological features. By comparing various aspects of these mouse models, specific and useful characteristics in a suitable model of MS-associated liver diseases, including hepato-carcinogenesis, can be highlighted.

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“…Several studies have described the applicability of the L-FABP protein to the detection of hepatic injury in diseases such as hepatocellular adenoma 12 and chronic HCV infection 13 .…”

Section: Groupsmentioning

confidence: 99%

L-FABP and I-FABP expression in newborn rats changes inversely in the model of necrotizing enterocolitis

et al. 2014

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Brazil. Mentor, conception and design of the study, manuscript writing and critical revision. ABSTRACT PURPOSE:To determine the expression of hepatic L-FABP and intestinal I-FABP in an experimental model of necrotizing enterocolitis (NEC) in neonatal rats. METHODS:Newborn Sprague-Dawley rats were divided into four groups: Control (C1) -exclusive breastfeeding at the first and sixth procedures (C6), NEC1 -fed formula milk and submitted to hypoxia and hypothermia at the first and sixth procedures (NEC6). The newborn pups were fed twice a day for three days, for a total of six procedures. Samples were collected for morphometric evaluation (body weight, liver weight, liver weight/body weight ratio, intestinal weight and intestinal/body weight ratio) and for immunohistochemical and Western blotting analysis. The values obtained were analyzed statistically, with the level of significance set at p<0.05. RESULTS:Morphometric measurements showed reduction of body and liver weights in the NEC group (p<0.05). Both immunohistochemistry and western blotting revealed that L-FABP expression in the liver was decreased and I-FABP expression in the ileum was increased in the NEC group (p<0.05).CONCLUSION: L-FABP and I-FABP expression changed inversely in the rat NEC model. These findings can contribute to a better diagnosis of NEC in human newborns.

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Histological and Immunohistochemical Revision of Hepatocellular Adenomas: A Learning Experience

Cited by 25 publications

References 28 publications

Preserved or enhanced OATP1B3 expression in hepatocellular adenoma subtypes with nuclear accumulation of β‐catenin

Preserved or enhanced OATP1B3 expression in hepatocellular adenoma subtypes with nuclear accumulation of β‐catenin

Animal models for analyzing metabolic syndrome‐associated liver diseases

L-FABP and I-FABP expression in newborn rats changes inversely in the model of necrotizing enterocolitis

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