Histological and immunohistochemical characterization of Hypoderma lineatum (Diptera: oestridae) warbles

Cabanelas, Eva; Fontán, Rosario Panadero; Fuertes, M.; Fernández, Miguel; Benavides, Julio; López, C.; Pérez-Creo, Ana; Díaz, Pablo; Morrondo, P.; Díez‐Baños, P.; Pérez, Valentín Pérez

doi:10.1016/j.vetpar.2015.06.017

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…CD3+ T lymphocytes constituted the major populations of the outer layer of the granulomas, this result is in agreement with the high number of this cell type in granulomas associated to remnants of the dead parasite Hypoderma lineatum but they were scarce in the periphery of viable larvae [45] and in the periphery of granulomatous cholangitis associated to the trematode Campula spp. in cetaceans [34].…”

Section: Lfsupporting

confidence: 86%

“…This suggest that CD3+ T lymphocytes play a role in the local host response, either by producing cytokines to induce macrophage activation or tissue repair. B lymphocytes and plasma cells expressing IgGlambda light chain were also abundant in the peripheral layer of the granulomas, suggesting a strong local humoral response, also reported in other parasitic granulomas [34,45].…”

Section: Lfsupporting

confidence: 67%

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A Partially Protective Vaccine for Fasciola hepatica Induced Degeneration of Adult Flukes Associated to a Severe Granulomatous Reaction in Sheep

Molina-Hernández

Ruiz-Campillo

Martínez-Moreno

et al. 2021

Animals

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Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since current anthelmintic therapy is no longer sustainable. A better knowledge of the host-parasite interaction is needed for the design of effective vaccines. The present study evaluates the microscopical hepatic lesions in sheep immunized with a partially protective vaccine (VAC1), a non-protective vaccine (VAC2), and an infected control group (IC). The nature of granulomatous inflammation associated with degeneration of adult flukes found in the VAC1 group was characterized by immunohistochemistry. Hepatic lesions (fibrous perihepatitis, chronic tracts, bile duct hyperplasia, infiltration of eosinophils and lymphocytes and plasma cells) were significantly less severe in the VAC1 group than in the IC group. Dead adult flukes within bile ducts were observed only in the VAC1 group and were surrounded by a severe granulomatous inflammation composed by macrophages and multinucleate giant cells with a high expression of lysozyme, CD163 and S100 markers, and a low expression of CD68. Numerous CD3+ T lymphocytes and scarce infiltrate of FoxP3+ Treg and CD208+ dendritic cells were present. This is the first report describing degenerated flukes associated to a severe granulomatous inflammation in bile ducts in a F. hepatica vaccine trial.

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Section: Lfsupporting

confidence: 86%

Section: Lfsupporting

confidence: 67%

A Partially Protective Vaccine for Fasciola hepatica Induced Degeneration of Adult Flukes Associated to a Severe Granulomatous Reaction in Sheep

Molina-Hernández

Ruiz-Campillo

Martínez-Moreno

et al. 2021

Animals

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“…Consistently, within different ages, it was evidence that younger animals, less than 3 years old, 54.7% higher than older animals, more than 3 years old, 16% (14,20,21) Our interpretation, the softer skin in younger animals which enable L1 to penetrate the skin easily than thicker skin in older animals, and that exactly what have been reported by (22). Alternatively, repeated exposure to hypodermosis would develop immunity by increasing IgG intensity after frequent exposure to infestation (23). Therefore, larvae1 might die in previously infested cattle due to skin defense mechanisms, which has been illustrated by previous investigations (2,24).…”

Section: Discussionsupporting

confidence: 58%

Sero-prevalence of hypodermosis in cattle in Mosul city, Iraq

Alhamdany¹,

Alhayali²

2022

IJVS

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Bovine hypodermosis has been known as a veterinary disease that mainly caused by Hypoderma bovis and Hypoderma lineatum. This study aimed to determine seroprevalence of cattle grubs in Mosul, Iraq utilizing indirect ELISA as well as effect of some risk factors on prevalence of hypodermosis. A total of two hundred blood specimens were collected from healthy and infested cattle through direct palpation. All samples were collected from cattle at Teaching Veterinary Hospital, Mosul abattoir, Kokjali and Bazwaya region during October 2020 to March 2021. Outcomes of iELISA showed 40.5% as an infestation rate of hypodermosis. Notably, infestation intensity has distributed as light, moderate and sever 11, 18.5 and 70.3% respectively. Interestingly, highest seroprevalence of hypodermosis was recorded in October 67.7%, and the lowest was recorded in February and March 22.2%, 25% respectively. Observations of current study also recorded the infection rate in age was 54.7% in animals less than 3 years old, and the lowest was 16% in animals more than 3 years old. Moreover, breed showed a significant risk factor 53.8% for imported cattle and 14.7% for local breed. Importantly, risk factor concerning area recorded the highest rate in Kokjali 52.2%. In contrast, the lowest rate recorded in Teaching Veterinary Hospital 14.8%. The present study is the first investigation to identify cattle hypodermosis by iELISA in Mosul, Iraq. iELISA has been approved as the best diagnostic tool for early detection of hypodermosis during the migrating phase. Interestingly, age, breed and months of study were significant risk factors.

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