Histological and Immunohisto-chemical Study of Toxic Effect of Gibberellic Acid Postnatally on Renal Cortex of Albino Rats

Husseiny, Nancy; Farag, Azza Ismael; Mohammed, Heba Osama

doi:10.21608/ejh.2020.21044.1214

Cited by 4 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These findings were confirmed also by morphometric analysis. These results were in consistence with Hassan et al, (2020) [47] who examined the toxic effect of gibberellic acid postnatally on renal cortex of albino rats.…”

Section: Discussionsupporting

confidence: 88%

Effect of Perfluorooctane Sulfonate on the Renal Cortex of Adult Male Albino Rats and the Possible Ameliorating Role of Quercetin

Amin,

Agaga

2022

Journal of Medical Histology

View full text Add to dashboard Cite

Introduction: Perfluorinated chemicals (PFCs) are materials that have many important industrial applications. Quercetin is a unique bioflavonoid that known with its antioxidant activity. Aim: To evaluate the histopathological effect of (PFOS) on rat renal cortex and the possible protective role of Quercetin. Materials and Methods: 45 adult male albino rats were divided into three groups, each contained 15 rats. The first group was the control group (I), which was splitted into three equal subgroups. The second group was (PFOS) receiving group (II) at a dose of 20mg /kg/day by gavage for 28 days. The third group was the protective group (III) which received the same dose of (PFOS) as group (II) in combination with Quercetin at a dose of 50 mg/kg b.w orally for 28 days. At the end of the study, the kidneys were dissected out and prepared for light microscopic examination. Results:The kidney sections of (PFOS) receiving group showed distortion of normal histology in the form of variations in the size and shape of renal glomeruli, peri glomerular inflammatory cell infiltrations, Disorganized renal tubules with dark stained (pyknotic) nuclei, cystic luminal dilatation and cytoplasmic vacuolations. In addition, some cortical convoluted tubules showed areas of interstitial hemorrhage, necrosis and exfoliation of tubular epithelial cells. Increased collagen fibers deposition and immune expression of PCNA and INOS stained sections. All these findings were improved by co-administration of Quercetin. Conclusion: Quercetin played an important role in improving the toxic effects of (PFOS) on kidney tissue of adult male rats.

show abstract

Section: Discussionsupporting

confidence: 88%

Effect of Perfluorooctane Sulfonate on the Renal Cortex of Adult Male Albino Rats and the Possible Ameliorating Role of Quercetin

Amin,

Agaga

2022

Journal of Medical Histology

View full text Add to dashboard Cite

show abstract

“…These leucocytic infiltrations were thought to be an immunological response of the organs to any potentially harmful substance. The findings are consistent with those made by Husseiny et al 2020, who discovered that GA3 can have harmful effects on a variety of mesenchymal organs, including the liver.…”

Section: A B C Dsupporting

confidence: 91%

“…For light microscopic study, liver and kidney specimens were fixed in 10% formalin saline for histo-pathological examination using H&E stain by following the method described by Melo et al ( 2020) and (Husseiny et al, 2020).…”

Section: -Histologic Studymentioning

confidence: 99%

“…Toxins are concentrated in the filtrate, moved through the tubular cells by the kidney, and some of them are even bioactivated. The kidneys are highly susceptible to a number of disorders as a result of all these functions (Husseiny et al, 2020). Interstitial fibrosis and tubular atrophy are two renal tubule disorders (increased with progressive glomerular damage and loss).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Toxic Effects of Chronic Use of Gibberellic Acid on Liver and Kidney and Hematological Study in Male Albino Rats

Bayoumy,

Abd El Salam,

ali

et al. 2023

Zagazig Journal of Forensic Medicine

View full text Add to dashboard Cite

Introduction: Gibberellic acid (GA3) plays important roles in many cellular processes including promoting stem elongation, and mobilization of food reserves in grass seed germination, juvenility, and sex expression. The aim of the work: To detect the possible toxic effects of GA3 on liver and kidney histopathology and effect on indicators of oxidative stress levels. Material and Methods: Seventy-five rats were included in the study and divided into two groups of animals (50 rats in treatment group and 25 rats in recovery group). The first group (treatment group) divided into 5 equal subgroups of rats (10 rats each) which were negative control group (G0T) received only distilled water, positive control treatment subgroup (G1T) received diluted sodium hydroxide in distilled water which are vehicle for GA3, subgroup G2T received 1/10 of LD50 (630 mg/kg) of GA3, subgroup G3T received 1/20 LD50 (315 mg/kg) of GA3, and subgroup G4T received 1/40 LD50 (157 mg/kg) of GA3. Treatment groups treated with GA3 orally by gavage for 12 weeks then salvaged by decapitation. The second group (recovery group) containing 25 rats that were treated with same manner as treatment groups for 12 weeks then kept after the treatment for recovery period for 2 weeks then salvaged to detect its cumulative effect. Recovery group divided into 5 equal subgroups (5 rats in each group) which were negative control recovery group (G0R), positive control recovery subgroup (G1R), subgroup G2R, subgroup G3R, and subgroup G4T. In all subgroups, liver and kidney tissue samples were taken to measure level of hepatic MDA concentration, hepatic catalase activity and examine hepatic and renal histopathologic changes. Results: hepatic MDA was significantly increased in all treatment and recovery groups in comparison to both control groups and level was directly proportional to dose of GA3 administered. There was significant reduction in the hepatic catalase enzyme activity in all treatment groups than the control treatment group especially in the group of highest doses (G2T). After the recovery period, the hepatic catalase enzyme activity continued to drop than its level after the treatment period, especially in the group (G2R). Histopathological examination revealed marked necrosis of tissues of, and the recovery period couldn't restore these pathological changes due to the cumulative effects of GA3 on the liver and kidneys. Conclusion and recommendation: GA3 possess risk to human health due to oxidative damage to tissues such as liver and kidney. Use of GA3 should be cautious, and its level in agricultural products should be regularly controlled.

show abstract

“…Por otro lado, manifestaciones nefrotóxicas se observaron en ratas en periodo de gestación y lactancia por consumo de 55 gác. giberelico Kg -1 rata (Husseiny et al, 2020).…”

Section: Issn Online 2007-9621unclassified

Análisis del ácido giberélico desde la cartografía conceptual con enfoque bioético y sustentable

Soto¹,

Acosta²,

Orduño-Cruz³

2022

Acta Universitaria

View full text Add to dashboard Cite

El ácido giberélico permite el aumento del rendimiento, el control de diferentes etapas del desarrollo y el estrés abiótico en cultivos. Diversas investigaciones sugieren que la exposición de animales de laboratorio al ácido giberélico podría estar asociada al mal funcionamiento de órganos. El objetivo de este trabajo fue identificar, desde la bioética y la sustentabilidad, los beneficios y perjuicios del uso del ácido giberélico mediante la cartografía conceptual y la socioformación. Para ello, se desarrollaron los ocho ejes de la cartografía conceptual. Se encontró que existe interés en la producción y aplicación sustentable del ácido giberélico; sin embargo, no se atiende a los principios de la bióetica. Por esta razón, se concluyó que el enfoque bioético y sustentable en el uso del ácido giberélico debe introducirse desde la formación de los profesionales del campo para generar conocimientos que ayuden a desarrollar formulaciones eficientes y selectivas.

show abstract

Histological and Immunohisto-chemical Study of Toxic Effect of Gibberellic Acid Postnatally on Renal Cortex of Albino Rats

Cited by 4 publications

References 0 publications

Effect of Perfluorooctane Sulfonate on the Renal Cortex of Adult Male Albino Rats and the Possible Ameliorating Role of Quercetin

Effect of Perfluorooctane Sulfonate on the Renal Cortex of Adult Male Albino Rats and the Possible Ameliorating Role of Quercetin

Toxic Effects of Chronic Use of Gibberellic Acid on Liver and Kidney and Hematological Study in Male Albino Rats

Análisis del ácido giberélico desde la cartografía conceptual con enfoque bioético y sustentable

Contact Info

Product

Resources

About