Histological and Histopathological Evaluation of the Testis

Russell, Lonnie D.; Ettlin, Robert A.; Hikim, Amiya P. Sinha; Clegg, Eric D.

doi:10.1111/j.1365-2605.1993.tb01156.x

Cited by 1,150 publications

(1,695 citation statements)

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“…After 30 min, approximately 10% from each pole of a testis was removed and placed in 5 ml of fresh Bouin's ®xative at 48C for 24 h. After washing with 70% ethanol the testes were processed for embedding in para n wax. Several sections (4 mm) were mounted onto microscopic slides and stained with hematoxylin-eosin and visualized by light microscopy as described (Russell et al, 1990).…”

Section: Histocytochemistrymentioning

confidence: 99%

Functional role for the c-Abl tyrosine kinase in meiosis I

et al. 1998

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The c-Abl tyrosine kinase is activated by ionizing radiation and certain other DNA-damaging agents. The DNA-dependent protein kinase (DNA ± PK) and the ataxia telangiectasia mutated (ATM) gene product, e ectors in the DNA damage response, contribute to the induction of c-Abl activity. The present study demonstrates that c-Abl is expressed in mouse and rat testes, and predominantly in pachytene spermatocytes of meiosis I. The results also demonstrate that c-Abl interacts directly with meiotic chromosomes. In concert with a requirement for c-Abl at the pachytene stage, we show that, in contrast to wild-type mice, testes from Abl 7/7 mice exhibit defects in spermatogenesis. These ®ndings provide the ®rst demonstration that c-Abl plays a functional role in meiosis.

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Section: Histocytochemistrymentioning

confidence: 99%

Functional role for the c-Abl tyrosine kinase in meiosis I

et al. 1998

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“…These junctions constitute the major component of the blood-testis barrier, and this barrier creates an immunoprivileged environment within the seminiferous tubules. When spermatogonia enter meiosis and become spermatocytes, the tight junctions are broken down and reformed to allow spermatocytes to translocate apically, complete meiosis and differentiate into spermatids in the immunoprivileged adluminal portion of the seminiferous tubules 1 . Consequently, when germ cells are introduced into recipient seminiferous tubules during the germ cell transplantation procedure, spermatogonial stem cells translocate in a basal direction through the tight junctions, towards the basement membrane of the seminiferous tubule.…”

Section: Germ Cell Transplantation and Colonizationmentioning

confidence: 99%

“…Spermatogenesis is a dynamic, continuous process in which diploid spermatogonia undergo ten mitotic and two meiotic divisions to differentiate into haploid spermatozoa 1 . This process generates a remarkable number of mature sperm -200 million per day in the human male -and requires an intimate association of germinal cells and somatic Sertoli cells within the seminiferous tubules of the testis.…”

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confidence: 99%

Germ cell transplantation and the study of testicular function

McLean

Johnston

Russell

et al. 2001

Trends in Endocrinology & Metabolism

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“…Mammalian spermatozoan development is a complex process that involves the proliferation and differentiation of spermatogonia, the meiosis of spermatocytes, and drastic morphological changes that occur during the progression from round spermatids to mature sperm [1]. Complete understanding of mammalian spermatogenesis is not easy because we have still not identified the structure or function of many of the genes that are involved in this process.…”

Section: Introductionmentioning

confidence: 99%

“…The five most frequent domains are the C2H2, PHD finger, KRAB-box, LIM and RING finger domains. RING finger proteins are characterized by the presence of a cysteine-rich domain (CX2CX CX [1][2][3]HX [2][3] CX2CX CX2C) and are involved in many biological processes, including signal transduction, transcriptional regulation, apoptosis and ubiquitination [5][6][7]. npg As one of the most versatile cellular regulatory mechanisms, ubiquitination is involved in many physiological and pathological events, including spermatogenesis, fertilization and sperm quality control [8,9].…”

Section: Introductionmentioning

confidence: 99%

Human RING finger protein ZNF645 is a novel testis-specific E3 ubiquitin ligase

Bai¹,

Zhang²,

Su³

et al. 2010

Asian J Androl

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A large number of testis-specific genes are involved in the complex process of mammalian spermatogenesis. Identification of these genes and their roles is important for understanding the mechanisms underlying spermatogenesis. Here we report on a novel human RING finger protein, ZNF645, which contains a C3HC4 RING finger domain, a C2H2 zinc-finger domain, and a proline-rich region, indicating that it has a structure similar to that of the c-Cbl-like protein Hakai. ZNF645 was exclusively expressed in normal human testicular tissue. Immunohistochemical analysis confirmed that ZNF645 protein was present in spermatocytes, round and elongated spermatids, and Leydig cells. Immunofluorescence staining of mature sperms further showed that the ZNF645 protein was localized over the postacrosomal perinuclear theca region and the entire length of sperm tail. An in vitro ubiquitination assay indicated that the RING finger domain of the ZNF645 protein had E3 ubiquitin ligase activity. Therefore, we suggest that ZNF645 might act as an E3 ubiquitin-protein ligase and play a role in human sperm production and quality control.

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Histological and Histopathological Evaluation of the Testis

Cited by 1,150 publications

References 0 publications

Functional role for the c-Abl tyrosine kinase in meiosis I

Functional role for the c-Abl tyrosine kinase in meiosis I

Germ cell transplantation and the study of testicular function

Human RING finger protein ZNF645 is a novel testis-specific E3 ubiquitin ligase

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