Histological and histochemical analyses of the cuttlebone sac of the golden cuttlefish Sepia esculenta

Abstract

The secretion function of mantle is closely related to shell formation in some bivalves and gastropods. Up to now, few researches have been reported for cuttlebone formation in the class Cephalopoda. In this study, the structure and secretion function of cuttlebone sac of the golden cuttlefish Sepia esculenta was analyzed using the histological and histochemical methods. The results showed that high and columnar cells located in sac epithelium, and flat cells existed near the base membrane. A lot of fibroblasts were found in the lateral mantle collective tissue. Some mucus, mucopolysaccharide and alkaline phosphatase (ALP) were found in the sac. The ultrastructural characteristics of Quasi-connective-tissue-calcium cells (QCTCC) were observed using a transmission electron microscope (TEM). The relationship between cuttlebone sac secretion function and shell formation was discussed.

Characterization of metal removal by os sepiae of Sepiella maindroni Rochebrune from aqueous solutions

“…NaOH is generalized to be a typical industrial catalyst [16] and has deprotonation effects [17]. Additionally, the OS contains neutral mucus, plenty of neutral mucopolysaccharide and alkaline phosphatase [18]. The structures of mucopolysaccharides are easily changed in alkali solution [19].…”

Effects of temperature fluctuations on cuttlebone formation of cuttlefish Sepia esculenta

“…Tang and Chai (1981) pointed out that there are numerous fi broblasts in shell sac, whose functions are related to wound repair and calcium salt crystal deposition (Zheng et al, 2007). These fi ndings indicate that shell sac probably plays an important role in lamellar deposition.…”