Histological and electron microscopy observations on the testis and spermatogenesis of the butterfly Dione juno (Cramer, 1779) and Agraulis vanillae (Linnaeus, 1758) (Lepidoptera: Nymphalidae)

Mari, Isabelle Pereira; Gigliolli, Adriana Aparecida Sinópolis; Nanya, Satiko; Portela-Castro, Ana Luiza de Brito

doi:10.1016/j.micron.2018.03.004

Cited by 19 publications

(17 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kuroda, 1974; Malawey, Mercati, Love, & Tomberlin, 2019; Ponlawat & Harrington, 2007). However, various studies suggest that lepidopteran males often stop producing eupyrene sperm after pupation (Chaudhury & Raun, 1966; Friedländer, 1997; Lachance & Olstad, 1988; Witalis & Godula, 1993; review in Friedländer et al, 2005; Mari, Gigliolli, Nanya, & Portela‐Castro, 2018). There are a few exceptions, though, for example, in the moths Calpodes ethlius (Lai‐Fook, 1982), Achroia grisella (Fernandez‐Winckler & Cruz‐Landim, 2008) and Galleria mellonella (Bebas et al, 2018) and a butterfly Polygonia c‐aureum (Hiroyoshi et al, 2017), spermatogenesis still occurs during the adult stage following certain stimuli such as larval diapause (Bebas et al, 2018) and adult overwintering (Hiroyoshi et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Combined cues of male competition influence spermatozoal investment in a moth

et al. 2020

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Combined cues of male competition influence spermatozoal investment in a moth

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The spermatogenesis of T. klimeschi occurs in cyst as in most insects [6,7,[21][22][23]. Before spermiogenesis, the characters of T. klimeschi is the low electron-dense band comprised by smooth endoplasmic reticulum in the cytoplasm of spermatocytes, compared to Euptoieta hegesia [24], Agraulis vanillae [25], Tribolium castaneum [23] and Timema poppensis [26]. In addition, its spermatogonia has a large and irregular nucleus, that is similar to T. poppensis [26] but different from A. vanillae [25].…”

Section: Discussionmentioning

confidence: 99%

“…Before spermiogenesis, the characters of T. klimeschi is the low electron-dense band comprised by smooth endoplasmic reticulum in the cytoplasm of spermatocytes, compared to Euptoieta hegesia [24], Agraulis vanillae [25], Tribolium castaneum [23] and Timema poppensis [26]. In addition, its spermatogonia has a large and irregular nucleus, that is similar to T. poppensis [26] but different from A. vanillae [25]. In the process of spermiogenesis, the nuclear chromatin undergoes different course of condensation in all species; sometimes the central zone, and other times the periphery, is the first to condense [2], both of these situations exist in this study.…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: The Male Reproductive Structure and Spermatogenesis of Trypophloeus Klimeschi Eggers (Coleoptera: Curculionidae: Scolytinae)

Gao

Dai

et al. 2019

Preprint

View full text Add to dashboard Cite

Background Trypophloeus Klimeschi Eggers (Coleoptera: Curculionidae: Scolytinae) is one of the most destructive pests of Populus alba var. pyramidalis (Bunge), resulting in significant losses in economic, ecological and social benefits in China’s northwest shelter forest. But research of reproductive system, spermiogenesis and spermatozoon ultrastructure of T. klimeschi that is basis of phylogeny, reproductive biology and controlling is still black. Results The male reproductive organ of T. klimeschi is composed of testis, seminal vesicle, strand shaped accessory gland containing long branch of strand shaped accessory gland and short branch of strand shaped accessory gland, curly accessory gland, vas deferens and a common ejaculatory duct. The number of sperm per cyst is 350~512. Its spermatozoon is slender, measuring about 75 μm in length and 0.5 μm in wide and composed of a 3-layred acrosomal complex, a nucleus with two different states of aggregation, two mitochondrial derivatives with dark crystal, a 9+9+2 axoneme that run more or less parallel to mitochondrial derivatives, two crystalline accessory bodies with a big compact “puff”-like expansion. Especially in the seminal vesicle, its long flagella folded into several turns and the whole sperm is wrapped in a film.Conclusion The general morphology of male reproductive tract, the spermatogenesis and the spermatozoa of T. klimeschi are, for the most part, similar to the majority of the Curculionidae. However, some distinct differences were found: the low electron-dense band in the cytoplasm of spermatocytes; two different aggregation states of spermatozoon nucleus; especially the stored way of T. klimeschi spermatozoa.

show abstract

“…During the prepupal or pupal period, the two separated testes fuse together to form a single one [6][7][8]. The testicular fusion has been reported in many lepidopteran insects, such as the Crambidae insects Diatraea saccharalis [7], the Lymantriidae insect Agraulis vanillae [8], the Nymphalidae insect Dione juno [8], the Noctuidae insects Heliothis zea [9], Heliothis virescens [10], and S. litura [11] and Ostrinia nubilalis [12], the Sphingidae insect Manduca sexta [13]. Most of these insects are important agricultural pests, causing extensive damage to cotton, soybean, tobacco, cruciferous vegetables [14,15].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the testicular fusion in Spodoptera litura

Chang

Liu

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

Background: Lepidoptera is one group of the largest plant-feeding insects and Spodoptera litura (Lepidoptera: Noctuidae) is one of the most serious agricultural pests in Asia countries. An interesting and unique phenomenon for gonad development of Lepidoptera is the testicular fusion. Two separated testes fused into a single one during the larva-to-pupa metamorphosis, which is believed to contribute to sperm production and the prevalence in field. To study the molecular mechanism of the testicular fusion, RNA sequencing (RNA-seq) experiments of the testes from 4-day-old sixth instar larvae (L6D4) (before fusion), 6-day-old sixth instar larvae (L6D6, prepupae) (on fusing) and 4-day-old pupae (P4D) (after fusion) of S. litura were performed. Results: RNA-seq data of the testes showed that totally 12,339 transcripts were expressed at L6D4, L6D6 and P4D stages. A large number of differentially expressed genes (DEGs) were up-regulated from L6D4 to L6D6, and then more genes were down-regulated from L6D6 to P4D. The DEGs mainly belongs to the genes related to the 20E signal transduction pathway, transcription factors, chitin metabolism related enzymes, the families of cytoskeleton proteins, extracellular matrix (ECM) components, ECM-related protein, its receptor integrins and ECM-remodeling enzymes. The expression levels of these genes that were up-regulated significantly during the testicular fusion were verified by qRT-PCR. The matrix metalloproteinases (MMPs) were found to be the main enzymes related to the ECM degradation and contribute to the testicular fusion. The testis was not able to fuse if MMPs inhibitor GM6001 was injected into the 5th abdomen region at L6D6 early stage. Conclusions: The transcriptome and DEGs analysis of the testes at L6D4, L6D6 and P4D stages provided genes expression information related to the testicular fusion in S. litura. These results indicated that cytoskeleton proteins, ECM-integrin interaction genes and ECM-related proteins were involved in cell migration, adhesion and fusion during the testicular fusion. The ECM degradation enzymes MMPs probably play a critical role in the fusion of testis.

show abstract

Histological and electron microscopy observations on the testis and spermatogenesis of the butterfly Dione juno (Cramer, 1779) and Agraulis vanillae (Linnaeus, 1758) (Lepidoptera: Nymphalidae)

Cited by 19 publications

References 21 publications

Combined cues of male competition influence spermatozoal investment in a moth

Combined cues of male competition influence spermatozoal investment in a moth

WITHDRAWN: The Male Reproductive Structure and Spermatogenesis of Trypophloeus Klimeschi Eggers (Coleoptera: Curculionidae: Scolytinae)

Transcriptomic analysis of the testicular fusion in Spodoptera litura

Contact Info

Product

Resources

About