Histological and Biochemical Studies on Cartilage From Osteoarthrotic Femoral Heads With Special Referencel To Surface Characteristics

Pd, Byers; Maroudase, A; Oztop, Fikri; Ra, Stockwell; Mf, Venn

doi:10.3109/03008207709152611

Cited by 64 publications

(25 citation statements)

References 11 publications

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“…4b, c). Collagen concentration per dry weight of normal bovine patellar superficial zone articular cartilage (from 49 to 62%) was at about the same level as the values obtained for full-thickness normal human femoral head articular cartilage (from 56 to 76%) [Byers et al, 1977;Venn, 1979] or human patellar articular cartilage (52%) [Väätäinen et al, 1998]. The water content of normal human femoral head articular cartilage (71%) was lower than in this study (from 83 to 85%) [Byers et al, 1977], which probably is attributable to the differences in water content in different species and joints.…”

Section: Discussionsupporting

confidence: 59%

Collagenase-Induced Changes in Articular Cartilage as Detected by Electron-Microscopic Stereology, Quantitative Polarized Light Microscopy and Biochemical Assays

Långsjö¹,

Rieppo²,

Pelttari³

et al. 2002

Cells Tissues Organs

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The purpose of this study was to compare the ability of electron-microscopic (EM) stereology with quantitative polarized light microscopy (PLM) and biochemical collagen (hydroxyproline) and crosslink (pyridinoline) analyses to detect changes in the superficial collagen network of bovine articular cartilage after digestion in vitro with purified bacterial (Clostridium histolyticum) collagenase (30 U/ml) for 24 and 48 h. Collagen volume (V_V) and surface (S_V) densities of the uppermost third of the superficial zone were estimated indirectly from zonal isotropic uniform random sections using collagen length density (L_V) and average collagen fibril diameter, or its average second power. Collagenase digestion caused a significant decrease in fibril diameter (64 to 62%), V_V (89 to 95%) and S_V (64 to 86%) after incubation for 24 and 48 h. Collagen L_V remained unchanged after 24 h incubation but decreased 63% after 48 h. Collagen concentration per dry weight, assayed biochemically from the whole superficial zone, decreased also significantly (29 to 60%) after 24 and 48 h digestions, respectively. The pyridinoline concentration per dry weight of the superficial zone decreased (31 to 57%) whereas the pyridinoline concentration per collagen remained unchanged. PLM revealed that the birefringence of the uppermost third of the superficial zone was decreased by 36% after digestion for 24 h though the total birefringence of the whole zone was not reduced. However, after 48 h, the birefringence of the whole superficial zone was significantly reduced (76%). All of the techniques compared in this study could detect collagen network degradation in bovine articular cartilage but the EM stereological technique was more sensitive at detecting the changes than PLM or biochemical assays.

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Section: Discussionsupporting

confidence: 59%

Collagenase-Induced Changes in Articular Cartilage as Detected by Electron-Microscopic Stereology, Quantitative Polarized Light Microscopy and Biochemical Assays

Långsjö¹,

Rieppo²,

Pelttari³

et al. 2002

Cells Tissues Organs

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show abstract

“…The histologic results presented are consistent with those found in numerous studies of human and experimental OA (20)(21)(22)(23)(24)(25)(26)(27). They show a general loss of intercellular matrix staining, but persistence of a strong pericellular reaction around most chondrocytes and cell clusters.…”

Section: Discussionsupporting

confidence: 89%

“…It is characterized in both spontaneous and experimentally induced disease by marked alterations in matrix histochemistry (20)(21)(22)(23)(24)(25)(26)(27) and ultrastructure (28-34), biochemical composition (3541), and cellular metabolism (21,35,(42)(43)(44). Variable rates of chondrocyte necrosis and reactive mitotic proliferation have also been reported (35,(45)(46)(47)(48)(49)(50).…”

Section: Discussionmentioning

confidence: 99%

Chondrons from articular cartilage. III. Morphologic changes in the cellular microenvironment of chondrons isolated from osteoarthritic cartilage

Poole

Matsuoka

Schofield

1991

Arthritis & Rheumatism

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Chondrons were isolated from human and canine osteoarthritic cartilage using low-speed homogenization techniques. Changes in chondron morphology were evaluated using differential interference-contrast microscopy, phase-contrast microscopy, and histochemical and ultrastructural methods. Chondrocyte viability was assessed using fluorescein diacetate staining, and chondron metabolism was investigated using autoradiography. The results suggest that initial changes in the collagen and proteoglycan distribution within the chondron are followed by chondrocyte proliferation to form clusters. These techniques offer the potential to study cell matrix interactions in degenerative osteoarthritis.Chondrocytes from hyaline cartilage are surrounded by a specialized microenvironment termed the chondron (1-3). In ultrastructural studies of mature canine and human articular cartilage, chondrons in the middle and deep layers were shown to consist of a single chondrocyte, a pericellular matrix rich in proteoglycans, and a pericellular capsule containing

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“…We therefore argue that it may be a relatively common phenomenon in joints generally. An increase in the water content of articular cartilage has been noted as one of the earliest features of fibrillation associated with osteoarthritis (Byers et al 1977 ;Aigner et al 1997 ;Chang et al 1997). The cause of this abnormal increase in hydration is still not fully understood.…”

Section: A Model Of Structural Transformation To Accommodate Large-scmentioning

confidence: 99%

Concerning the ultrastructural origin of large‐scale swelling in articular cartilage

Chen

Broom

1999

Journal of Anatomy

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The swelling behaviour of the general matrix of both normal and abnormally softened articular cartilage was investigated in the context of its relationship to the underlying subchondral bone, the articular surface, and with respect to the primary structural directions represented in its strongly anisotropic collagenous architecture. Swelling behaviours were compared by subjecting tissue specimens under different modes of constraint to a high swelling bathing solution of distilled water and comparing structural changes imaged at the macroscopic, microscopic and ultrastructural levels of resolution. Near zero swelling was observed in the isolated normal general matrix with minimal structural change. By contrast the similarly isolated softened general matrix exhibited large-scale swelling in both the transverse and radial directions. This difference in dimensional stability was attributed to fundamentally different levels of fibril interconnectivity between the 2 matrices. A model of structural transformation is proposed to accommodate fibrillar rearrangements associated with the large-scale swelling in the radial and transverse directions in the softened general matrix.

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Histological and Biochemical Studies on Cartilage From Osteoarthrotic Femoral Heads With Special Referencel To Surface Characteristics

Cited by 64 publications

References 11 publications

Collagenase-Induced Changes in Articular Cartilage as Detected by Electron-Microscopic Stereology, Quantitative Polarized Light Microscopy and Biochemical Assays

Collagenase-Induced Changes in Articular Cartilage as Detected by Electron-Microscopic Stereology, Quantitative Polarized Light Microscopy and Biochemical Assays

Chondrons from articular cartilage. III. Morphologic changes in the cellular microenvironment of chondrons isolated from osteoarthritic cartilage

Concerning the ultrastructural origin of large‐scale swelling in articular cartilage

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