Histological and bacteriological evaluation of digital dermatitis in cattle, with special reference to spirochaetes and <i>Campylobacter faecalis</i>

Döpfer, Dörte; Koopmans, A.K.; Meijer, F. A.; Szakáll, I.; Schukken, Y.H.; Klee, Wolfgang; Bosma, R. B.; Cornelisse, J. L.; Asten, Alphons J.A.M. van; Huurne, A.A.H.M. ter

doi:10.1136/vr.140.24.620

Cited by 267 publications

(361 citation statements)

References 7 publications

Supporting

Mentioning

320

Contrasting

Unclassified

Order By: Relevance

“…A recently discovered species, Guggenheimella bovis, has been suggested as a potential pathogen but has until now been isolated only from a few DD-infected animals (30,35). Still, the high number of invasive spirochetes observed suggests an active contribution of this group to DD pathogenesis (8,9,12,16,24,35). This hypothesis is further substantiated by the fact that serum samples from cattle infected with DD contain elevated levels of antibody to Treponema antigens (13,25,36).…”

mentioning

confidence: 57%

Evidence of Multiple Treponema Phylotypes Involved in Bovine Digital Dermatitis as Shown by 16S rRNA Gene Analysis and Fluorescence In Situ Hybridization

et al. 2008

View full text Add to dashboard Cite

The etiopathogenesis of the skin disease digital dermatitis (DD), an important cause of lameness in cattle, remains uncertain. Microscopically, the disease appears to be polymicrobial, with spirochetes as the predominant bacteria. The objective of this study was to identify the main part of the bacteria involved in DD lesions of cattle by using culture-independent molecular methods. Ten different phylotypes of Treponema were identified either by 16S rRNA gene sequencing of bacteria from DD lesions or by fluorescence in situ hybridization (FISH) analysis using phylotype-specific 16S rRNA-directed oligonucleotide probes. Two phylotypes, phylotype 1 (PT1) and PT2, were not closely related to any characterized treponemal species. PT7 was 99.3% identical to Treponema denticola, while PT9 resembled T. vincentii by 96%. The remaining phylotypes, PT3, PT4, PT5, PT6, and PT8, and Treponema brennaborense had previously been isolated from DD lesions. Forty DD biopsy specimens were examined for Treponema by FISH. With one exception, all of the biopsy specimens revealed epidermotropic, intermingled infection with three or more different phylotypes (mean, 4.7). The most prevalent species were PT1 (95%), PT6 (93%), and PT3 (85%). While colonization by PT3 was confined to the surface of the epidermis, both PT1 and PT6 invaded deep into the stratum spinosum and were seen in ulcerated dermal papillae. In two cases, all 10 phylotypes were demonstrated. Furthermore, FISH with a Treponema group-specific probe showed that Treponema accounted for more than 90% of the total bacterial population in the biopsy specimens. These data strongly suggest that a group of apparently symbiotic Treponema species are involved as primary bacterial pathogens in DD.

show abstract

mentioning

confidence: 57%

Evidence of Multiple Treponema Phylotypes Involved in Bovine Digital Dermatitis as Shown by 16S rRNA Gene Analysis and Fluorescence In Situ Hybridization

et al. 2008

View full text Add to dashboard Cite

show abstract

“…White line separation category definitions were mild: marks along the white line; moderate: deep, affected fissures; or severe: very deep, profound fissures with either involvement of the corium, purulent exudate, necrosis, granulation tissue, or separation of the wall (Sogstad et al, 2007). Region of the foot was identified as recommended by the standard foot map (Greenough and Vermunt, 1991), and digital dermatitis was classified according to the M-scale (Döpfer et al, 1997) including the M4.1 category .…”

Section: Claw Horn Disruption Lesion Assessmentmentioning

confidence: 99%

A prospective cohort study of digital cushion and corium thickness. Part 2: Does thinning of the digital cushion and corium lead to lameness and claw horn disruption lesions?

Newsome

Green

Bell

et al. 2017

Journal of Dairy Science

View full text Add to dashboard Cite

“…Indeed, various types of bacteria have been identified within BDD manifestations including spirochetes, Bacteroides species, Campylobacter species, Fusobacterium species, and Peptococcus species (2,3,10,18,25,28). The result of this complex bacteriological environment is that the exact etiology of BDD remains unresolved.…”

mentioning

confidence: 99%

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions

et al. 2009

View full text Add to dashboard Cite

This study used a PCR-based approach targeting 16S rRNA gene fragments to determine the occurrence and association of the three bovine digital dermatitis (BDD) treponeme phylogroups within lesions found in cattle from the United Kingdom. Examination of 51 BDD lesions collected from infected cattle across the United Kingdom revealed that BDD treponeme group 1 (Treponema medium/Treponema vincentii-like), group 2 (Treponema phagedenis-like), and group 3 (Treponema putidum/Treponema denticola-like) were present in 96.1%, 98%, and 76.5% of BDD lesions, respectively. The three phylogroups were present together in 74.5% of lesions. The PCR assays enabled the isolation of further treponeme strains from previously mixed primary BDD lesion cultures. Here a representative from each of the three distinct treponeme phylogroups was isolated from a single BDD lesion for the first time. These data highlight the extent to which this disease is polytreponemal. Immunohistochemistry and electron microscopy were used to investigate lesional hoof tissues, resulting in treponemes being identified copiously in hair follicles and sebaceous glands, suggesting a potential route of exit and/or entry for these pathogens. This study gives further evidence for the importance of the three treponeme groups in BDD pathogenesis and reiterates the value of molecular genetic approaches for isolating and identifying fastidious anaerobes.

show abstract

Histological and bacteriological evaluation of digital dermatitis in cattle, with special reference to spirochaetes and Campylobacter faecalis

Abstract: TABLE 1: Description of the four disease classes associated with the development of digital dermatitis (Dopfer 1994

Cited by 267 publications

References 7 publications

Evidence of Multiple Treponema Phylotypes Involved in Bovine Digital Dermatitis as Shown by 16S rRNA Gene Analysis and Fluorescence In Situ Hybridization

Evidence of Multiple Treponema Phylotypes Involved in Bovine Digital Dermatitis as Shown by 16S rRNA Gene Analysis and Fluorescence In Situ Hybridization

A prospective cohort study of digital cushion and corium thickness. Part 2: Does thinning of the digital cushion and corium lead to lameness and claw horn disruption lesions?

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions

Contact Info

Product

Resources

About