Histological Analysis of Microstructure of UASB Granules

Bilevel thresholding of floc images

“…The flocs saturated with xylene were immersed in molten paraffin at 65 • C overnight. Finally the paraffin-embedded cakes were cooled to 25 • C in peel-off molds and solidified to form blocks for slicing [13]. The block was then sliced into sections of thickness 5 µm using a microtome (Leitz Model 1400, Germany).…”

Structure of conditioned sludge flocs

“…The cake saturated with xylene was immersed in molten paraffin at 65 o C overnight. Finally, the paraffinembedded cakes were cooled to 25 C in peel-off molds and solidified to form blocks for slicing [21]. The block was then sliced into sections of thickness 5 mm using a microtome (Leitz Model 1400, Germany).…”

Effect of pre-hydrolysis on floc structure

“…The cake saturated with xylene was immersed in molten paraffin at 65 8C overnight. Finally, the paraffin-embedded cakes were cooled to 25 8C in peeloff molds and solidified to make blocks that could be sliced (Chui and Fang, 1994). Each block was then sliced into sections of thickness 5 mm using a microtome (Leitz Model 1400, Germany).…”