Histological Analysis of Human Meniscal Allografts

Rodeo, Scott A.; Seneviratne, Aruna; Suzuki, Katsunori; Felker, Kevin; Wickiewicz, Thomas L.; Warren, Russell F.

doi:10.2106/00004623-200008000-00002

Cited by 205 publications

(145 citation statements)

References 16 publications

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“…Before this investigation, the presence of MHC II had not been analyzed for isolated MCs, although researchers have stained native human and ovine meniscus tissue for MHC class II and found that MCs do not express MHC class II, whereas synovial and endothelial cells within the meniscus do. [47][48][49][50][51] These results were also confirmed through immunohistochemical staining of bovine and leporine meniscus tissues in this study. In agreement with native tissue investigations, the results from the present study suggest that meniscus tissue may be similar to articular cartilage when MHC II is not present on cells in situ, but upon isolation MHC II expression is enhanced.…”

Section: Discussionsupporting

confidence: 79%

Immunogenicity of Bovine and Leporine Articular Chondrocytes and Meniscus Cells

Huey

Willard

Athanasiou

2012

Tissue Engineering Part A

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Immune rejection is a major concern for any allogeneic or xenogeneic graft. For in vivo investigations of cartilage tissue engineering strategies, small animal models such as the leporine model are commonly employed. Many studies report little to no immune rejection upon allogeneic or xenogeneic implantation of native articular and meniscal cartilages. This study investigated whether bovine and leporine articular chondrocytes (ACs) and meniscus cells (MCs) have immunoprivileged characteristics because of their ability to stimulate proliferation of leporine peripheral blood mononuclear cells (PBMCs) in vitro. After 6 days of co-culture, none of the cell types caused a proliferative response in the leporine PBMCs, indicating that these cells may not elicit immune rejection in vivo. Reverse transcriptase polymerase chain reaction analysis for major histocompatibility complex class (MHC) I and II and costimulation factors CD80 and CD86 revealed that all cell types produced messenger RNA for MHC I and II, but only some were CD80 or CD86 positive, and none were positive for both costimulation factors. Flow cytometry found that bovine MCs and ACs displayed MHC II (MCs: 32.5%, ACs: 14.4%), whereas only leporine ACs were MHC II positive (7.5%). Although present in isolated cells, MHC I and II were not observed in intact bovine or leporine hyaline cartilage or meniscus tissues. Despite some presence of MHC II and costimulation factors, none of the cell types studied were able to cause PBMC proliferation. These findings indicate that bovine and leporine MCs and ACs share a similar immunoprivileged profile, bolstering their use as allogeneic and xenogeneic cell sources for engineered cartilage.

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Section: Discussionsupporting

confidence: 79%

Immunogenicity of Bovine and Leporine Articular Chondrocytes and Meniscus Cells

Huey

Willard

Athanasiou

2012

Tissue Engineering Part A

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“…The biomechanical function of the meniscal allograft depends not only on the quality of the surgical fixation technique, but also on the phenotype of the cell population of the allograft. It is not known if recipient meniscus-invading cells, probably derived from the synovial lining, have the same capacity to produce extracellular matrix as have native meniscus cells 8,34 . It was hypothesized at the start of this study that the use of viable meniscal allografts would be more beneficial than the use of acellular grafts (deep-frozen, lyophilized, or cryopreserved grafts).…”

Section: Discussionmentioning

confidence: 99%

Transplantation of Viable Meniscal Allograft

Verdonk

Demurie

Almqvist

et al. 2005

The Journal of Bone & Joint Surgery

261

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“…Significantly higher cellular viability and collagen organization were found on biopsy of the grafts secured by soft-tissue fixation only, which may be related to a higher immunologic host response caused by the addition of bone plugs. 6 There is also a risk of increased articular cartilage damage if the bone plugs are malpositioned. 7 A meta-analysis and clinical studies have shown comparable outcomes between the 2 different fixation techniques.…”

Section: Discussionmentioning

confidence: 99%

Arthroscopic Meniscal Allograft Transplantation With Soft-Tissue Fixation Through Bone Tunnels

Spalding

Parkinson

Smith

et al. 2015

Arthroscopy Techniques

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Meniscal allograft transplantation improves clinical outcomes for patients with symptomatic meniscus-deficient knees. We describe an established arthroscopic technique for meniscal allograft transplantation without the need for bone fixation of the meniscal horns. After preparation of the meniscal bed, the meniscus is parachuted into the knee through a silicone cannula and the meniscal horns are fixed with sutures through bone tunnels. The body of the meniscus is then fixed with a combination of all-inside and inside-out sutures. This technique is reliable and reproducible and has clinical outcomes comparable with those of bone plug fixation techniques.

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Histological Analysis of Human Meniscal Allografts

Cited by 205 publications

References 16 publications

Immunogenicity of Bovine and Leporine Articular Chondrocytes and Meniscus Cells

Immunogenicity of Bovine and Leporine Articular Chondrocytes and Meniscus Cells

Transplantation of Viable Meniscal Allograft

Arthroscopic Meniscal Allograft Transplantation With Soft-Tissue Fixation Through Bone Tunnels

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