Histological Analyses Reveal Promising Features in Coffea arabica Cell Suspension Culture

Abstract

Biotechnological techniques have been extensively studied to provide practical results for coffee improvement. Among these techniques, the present study deals with the somatic embryogenesis for crop improvement. Aims: To describe and correlate the morphology of Coffea arabica cell suspensions with their growth curve through electron microscopy and photonic analysis. Methodology: During 28 days of the culture period, samples of cell culture were collected at four days interval and the growth curve of the cell suspensions was performed by calculating the growth and the growth rate of the same. Cell suspension samples were used to perform the histological and ultrastructural analyses.