Histologic evaluation of the width of soft tissue necrosis adjacent to carbon dioxide laser incisions

Histologic evaluation of thermal damage produced on soft tissues by CO2, Er,Cr:YSGG and diode lasers

“…The literature describes different methodologies to measure it, such as measurement of temperature increase on the tissue (5,8), energy absorption by the target tissue (9,10) and the most common, the histological evaluation of the tissue next to the irradiated area (4,11,12). Most reports point out that the result of this temperature increase can be inferred from denaturalization and hyalinization of the tissue next to the irradiated area, and it can be assessed using conventional techniques of microscopy and standard staining of hematoxillin-eosin, which show an area of eosinophilic coagulation (1,12,13). In this study we used Masson trichromic staining because this technique differentiates without ambiguity thermally damaged collagen of unaffected tissue, as reported by several papers (14)(15)(16).…”

Pseudodysplastic epithelial artefacts associated with oral mucosa CO2 laser excision: an assessment of margin status

“…Using a carbon dioxide (CO 2 ) laser beam to procure diagnostic biopsy specimens is compromised by thermal cytological artefacts that include vacuolation of the superficial layer, detachment and shredding of keratin, basal cell degeneration, and separation from the lamina propia 9,23 . These artefacts could be critical when assessing dysplastic changes, as thermal damage induced along the lasertreated margins would simulate cytological atypia 6 .…”

Lasers and soft tissue: 'fixed' soft tissue surgery

“…This risk can exist through the penetration of short wavelengths or the conductive heat effects arising from long wavelength char that is super-heated. [5][6][7][8][9] It is advisable, therefore, to assess the thickness, vascularity and position of any target 6,[10][11][12] during the removal of a giant cell granuloma. Laser therapy has been cited as a possible modality in treating this condition.…”