Histologic comparison of vocal fold microflap healing with sutures and glue

Maunsell, Rebecca; Freitas, Leandro L. de; Altemani, Albina; Crespo, Agrício Nubiato

doi:10.1002/lary.23914

Cited by 11 publications

(15 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The authors concluded that microflap closure via secondary intension provided better postoperative voice outcome . Other studies have reported that the performance of fibrin glue for vocal fold wound closure is unsatisfactory …”

Section: Introductionmentioning

confidence: 94%

“…The results showed that the suture treatment group had the highest collagen concentration, and the fibrin treatment group had the highest number of inflammatory cells. The study concluded that neither method was effective in either reducing scar formation or promoting wound healing relative to the no‐treatment group …”

Section: Introductionmentioning

confidence: 97%

“…Consequently, they reported that fibrin glues may increase the risk of fibrinogenesis. 6 Maunsell et al 7 compared vocal fold microflap healing for groups treated with fibrin glue, sutures, and no treatment in a 90-day rabbit study in 2013. For each animal, microflap incisions were made bilaterally.…”

Section: Introductionmentioning

confidence: 99%

“…The study concluded that neither method was effective in either reducing scar formation or promoting wound healing relative to the no-treatment group. 7 Myer et al 8 measured the tensile strength of fibrin glue for vocal fold microflap wound closure in 2015 in comparison with that of sutures and no treatment. The animal models included six excised bovine vocal folds and five porcine vocal folds.…”

Section: Introductionmentioning

confidence: 99%

“…9 Other studies have reported that the performance of fibrin glue for vocal fold wound closure is unsatisfactory. [6][7][8] Recent developments in biomaterials and biotechnology have yielded a number of alternatives to fibrin glue. This motivated the current research work to identify, characterize, and test other surgical adhesives for vocal fold wound closure.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Investigation of surgical adhesives for vocal fold wound closure

et al. 2019

View full text Add to dashboard Cite

Objectives Surgical adhesives are increasingly used for vocal fold microsurgery to assist wound closure and reduce the risks of scar formation. Currently used vocal fold adhesives such as fibrin glue, however, have thus far not been found to promote wound closure or reduce scarring. The objectives of this study were to investigate the mechanical strength and the cytotoxicity of three commercially available adhesives (Glubran 2, GEM, Viareggio, Italy; BioGlue, CryoLife, Kennesaw, GA; and Tisseel, Baxter Healthcare, Deerfield, IL) for vocal fold wound closure. Methods Shear and tension tests were performed on 150 porcine larynges. The cytotoxicity of the adhesives to immortalized human vocal fold fibroblasts was investigated using neutral red uptake assays. Results The average shear adhesive strength for Tisseel, BioGlue, and Glubran 2 was 13.86 ± 5.03 kilopascal (kPa), 40.92 ± 17.94 kPa, and 68.79 ± 13.29 kPa, respectively. The tensile adhesive strength for Tisseel, BioGlue, and Glubran 2 was 10.70 ± 6.42 kPa, 34.27 ± 12.59 kPa, and 46.67 ± 12.13 kPa, respectively. The vocal fold cell viabilities in extracts of Tisseel, BioGlue, and Glubran 2 were 99.27%, 43.05%, and 1.79%, respectively. Conclusion There was a clear tradeoff between adhesive strength and toxicity. The maximum failure strength in shear or tension of the three surgical adhesives ranked from strongest to the weakest was: 1) Glubran 2, 2) BioGlue, and 3) Tisseel. Tisseel was found to be the least toxic of the three adhesives, whereas Glubran 2 was the most toxic. Level of Evidence NA Laryngoscope, 129:2139–2146, 2019

show abstract

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Investigation of surgical adhesives for vocal fold wound closure

et al. 2019

View full text Add to dashboard Cite

show abstract

Comparison of tensile strength of fibrin glue and suture in microflap closure

et al. 2014

View full text Add to dashboard Cite

show abstract

Characterizing Vocal Fold Injury Recovery in a Rabbit Model With Three‐Dimensional Virtual Histology

et al. 2020

View full text Add to dashboard Cite

Objectives/Hypothesis: In animal studies of vocal fold scarring and treatment, imaging-based evaluation is most often conducted by tissue slicing and histological staining. Given variation in anatomy, injury type, severity, and sacrifice timepoints, planar histological sections provide limited spatiotemporal details of tissue repair. Three-dimensional (3D) virtual histology may provide additional contextual spatial information, enhancing objective interpretation. The study's aim was to evaluate the suitability of magnetic resonance imaging (MRI), microscale computed tomography (CT), and nonlinear laser-scanning microscopy (NM) as virtual histology approaches for rabbit studies of vocal fold scarring.Methods: A unilateral injury was created using microcup forceps in the left vocal fold of three New Zealand White rabbits. Animals were sacrificed at 3, 10, and 39 days postinjury. ex vivo imaging of excised larynges was performed with MRI, CT, and NM modalities.Results: The MRI modality allowed visualization of injury location and morphological internal features with 100-μm spatial resolution. The CT modality provided a view of the injury defect surface with 12-μm spatial resolution. The NM modality with optical clearing resolved second-harmonic generation signal of collagen fibers and two-photon autofluorescence in vocal fold lamina propria, muscle, and surrounding cartilage structures at submicrometer spatial scales.Conclusions: Features of vocal fold injury and wound healing were observed with MRI, CT, and NM. The MRI and CT modalities provided contextual spatial information and dissection guidance, whereas NM resolved extracellular matrix structure. The results serve as a proof of concept to motivate incorporation of 3D virtual histology techniques in future vocal fold injury animal studies.

show abstract

Histologic comparison of vocal fold microflap healing with sutures and glue

Abstract: The use of microsutures or fibrin glue to close microflap defects did not consistently improve vocal fold healing nor produce better scarring results when compared to healing by second intention.

Cited by 11 publications

References 29 publications

Investigation of surgical adhesives for vocal fold wound closure

Investigation of surgical adhesives for vocal fold wound closure

Comparison of tensile strength of fibrin glue and suture in microflap closure

Characterizing Vocal Fold Injury Recovery in a Rabbit Model With Three‐Dimensional Virtual Histology

Contact Info

Product

Resources

About