Histologic Characterization of Human Ear Ossicles for the Development of Tissue-Engineered Replacements

D’Alessandro, Delfo; Danti, Serena; Vito, Andrea De; Forlì, Francesca; Bruschini, Luca; Berrettini, Stefano

doi:10.1097/mao.0b013e31826a527d

Cited by 10 publications

(8 citation statements)

References 22 publications

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“…Furthermore, tissue engineering can benefit from such advancements to develop new biomimetic and bioinspired materials to customize patient-specific devices to replace native tissues. , In view of this, a remarkable field of application is certainly the middle-ear prosthetics, including ossicular bones and tympanic membrane replacements, − in which all the structures possess abundant collagen and perform upon acoustic transient loads, to deliver more biocompatible collagen-based devices for long-term outcomes.…”

Section: Discussionmentioning

confidence: 99%

Wave Propagation and Energy Dissipation in Collagen Molecules

Milazzo

Jung

Danti

et al. 2020

ACS Biomater. Sci. Eng.

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Collagen is the key protein of connective tissue (i.e., skin, tendons and ligaments, cartilage, among others) accounting for 25% to 35% of the whole-body protein content, and entitled of conferring mechanical stability. This protein is also a fundamental building block of bone due to its excellent mechanical properties together with carbonated hydroxyapatite minerals. While the mechanical resilience and viscoelasticity have been studied both in vitro and in vivo from the molecule to tissue level, wave propagation properties and energy dissipation have not yet been deeply explored, in spite of being crucial to understand the vibration dynamics of collagenous structures (e.g., eardrum, cochlear membranes) upon impulsive loads. By using a bottom-up atomistic modelling approach, here we study a collagen peptide under two distinct impulsive displacement loads, including longitudinal and transversal inputs. Using a one-dimensional string model as a model system, we investigate the roles of hydration and load direction on wave propagation along the collagen peptide and the related energy dissipation. We find that wave transmission and energy-dissipation strongly depend on the loading direction. Also, the hydrated collagen peptide can dissipate five times more energy than dehydrated one. Our work suggests a distinct role of collagen in term of wave transmission of different tissues such as tendon and eardrum. This study can step towards understanding the mechanical behaviour of collagen upon transient loads, impact loading and fatigue, and designing biomimetic and bio-inspired materials to replace specific native tissues such as the tympanic membrane.

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Section: Discussionmentioning

confidence: 99%

Wave Propagation and Energy Dissipation in Collagen Molecules

Milazzo

Jung

Danti

et al. 2020

ACS Biomater. Sci. Eng.

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“…The ear ossicles are made of a special type of compact bone with a peculiar histological organization. This feature should be taken into consideration to achieve optimal sound conduction . Of the other bone tissue types in the human body, the cortical bones of the tibia and the femur have shown a very good histological similarity .…”

Section: Discussionmentioning

confidence: 99%

Ossicular replacement prostheses from banked bone with ergonomic and functional geometry

et al. 2016

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This study shows the fabrication of innovative ossicular replacement prostheses (ORPs) based on banked cortical bone using computer numerically controlled ultraprecision micromilling, as a substantial improvement of "second generation" ORPs. Our aim is to combine optimal middle ear compatibility and surgical manageability in a single device, by releasing off-the-shelf homograft ORPs provided with the appealing features of synthetic ORPs, such as lightness, safety, measurement accuracy, surface decoration, and geometric plasticity. The new total ORP prototype was 13.1 ± 0.1 mg, leading to 81% weight reduction with respect to the previous model. Surface motifs of the head plate were applied to prevent slipping and migration after surgery, as shown by finite element modeling analysis. In addition, bone ORPs were provided with holed head plates to facilitate their surgical positioning while reducing their mass. A comparative measurement of acoustic responses of bone against synthetic partial ORPs in the 250-8000 Hz frequency range demonstrated their superior behavior. This study showed that banked compact bone can be optimally manufactured, eventually enabling the fabrication of light, standardized, and highly performant ORPs. The new bone ORPs may represent the ideal combination of biocompatibility and technology which can ultimately accomplish unmet otosurgical needs. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2495-2506, 2017.

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“…D'Alessandro et al studied the detailed morphology of the ossicles for the purpose of assisting the creation of prostheses. 7 They found the presence of compact bone evident in the head, crura, and stapes footplate. Areas of cartilage were found in the top of the head and along the footplate.…”

Section: Discussionmentioning

confidence: 98%

Anatomopathology of the Superstructure of the Stapes in Patients with Otosclerosis

Carvalho¹,

Hamerschmidt

Telles

et al. 2014

Int. Arch. Otorhinolaryngol.

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Introduction Otosclerosis is a disease that causes bone resorption and deposition in the auditory structures, leading to deafness. Many studies have evaluated the histopathology of the stapes footplate in this disease (osteoblasts, osteoclasts, vascular proliferation, fibroblasts, and histiocytes), but we found no studies in the literature involving the histology of the superstructure of the stapes. Objectives To perform an analysis under optical microscopy of histopathologic findings of the superstructure of the stapes from patients with otosclerosis. Methods A contemporary cross-sectional cohort study of pathology analysis of superstructures of the stapes of patients with otosclerosis. Results Fifteen superstructures of stapes in patients with otosclerosis operated in our service and four stapes of cadavers used for dissection (controls) were evaluated. No areas of bone resorption or deposition or presence of osteoclasts and osteoblasts in the superstructure of the stapes were found. However, we found in the more distal portions of the crura areas with prominent cementitious lines and woven bone, which was different than the mature trabecular bone found in the head of the stapes or in the controls. Conclusion There were histologic changes in the superstructure of the stapes in patients with otosclerosis operated in our service.

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Histologic Characterization of Human Ear Ossicles for the Development of Tissue-Engineered Replacements

Cited by 10 publications

References 22 publications

Wave Propagation and Energy Dissipation in Collagen Molecules

Wave Propagation and Energy Dissipation in Collagen Molecules

Ossicular replacement prostheses from banked bone with ergonomic and functional geometry

Anatomopathology of the Superstructure of the Stapes in Patients with Otosclerosis

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