Histologic Changes of Doxycycline Pleurodesis in Rabbits

Hurewitz, Adam N.; Lidonicci, Ken; Wu, Chien Liang; Reim, David; Zucker, Stanley

doi:10.1378/chest.106.4.1241

Cited by 79 publications

(59 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In general, since SAHN and GOOD [1] wrote their initial paper on the effect of common sclerosing agents in rabbits, most studies of pleurodesis in experimental animals have been conducted in rabbits. Studies during the past 18 months have also used rabbits to examine pleurodesis [25,26]. In general, the results with the various sclerosing agents in rabbits have paralleled those in humans.…”

Section: -Macroscopic Examination Of the Right Sidementioning

confidence: 99%

Corynebacterium parvum versus tetracycline as pleural sclerosing agents in rabbits

Vargas

Wang²,

Teixeira³

et al. 1995

Eur Respir J

View full text Add to dashboard Cite

Tetracycline has been one of the most commonly used agents for producing a pleurodesis. However, it is no longer available due to more stringent requirements on the manufacturing process. The objective of this project was to determine whether Corynebacterium parvum is an effective sclerosant in an experimental model in rabbits. The following medications were instilled intrapleurally in anaesthetized male rabbits: tetracycline 35 mg.kg-1 or C. parvum 4 or 8 mg, all diluted with bacteriostatic saline solution. Twenty eight days after the instillation, the animals were sacrificed and the pleural spaces assessed macroscopically for evidence of pleurodesis and microscopically for evidence of fibrosis and inflammation. The intrapleural injection of C. parvum was ineffective in creating pleural fibrosis. The mean degree of pleurodesis in the 10 rabbits who received tetracycline was 3.5 +/- 0.7 (scale 0-4) whilst in the 10 rabbits that received 4 mg C. parvum it was 0.0 +/- 0.0, and in the 10 rabbits that received 8 mg C. parvum it was 0.5 +/- 0.8. Based on this study, we recommend that C. parvum should not be used as a pleural sclerosant in patients with normal pleura.

show abstract

Section: -Macroscopic Examination Of the Right Sidementioning

confidence: 99%

Corynebacterium parvum versus tetracycline as pleural sclerosing agents in rabbits

Vargas

Wang²,

Teixeira³

et al. 1995

Eur Respir J

View full text Add to dashboard Cite

show abstract

“…This animal model has been used since Sahn and Good [13] first wrote about the effect of common sclerosing agents. Several studies within the past few years have used rabbits to study pleurodesis [14][15][16]. In general, the results with the various sclerosing agents in rabbits have paralleled those in humans.…”

Section: Discussionmentioning

confidence: 99%

“…Its derivatives are also somewhat limited in their availability worldwide. In addition they produce intense pain in humans [3] and hemothorax in rabbits [10,15]. Talc is an excellent sclerosing agent but it has led to the development of acute respiratory distress syndrome in some patients [1,2].…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Ethanolamine Oleate as a Pleural Sclerosing Agent in Rabbits

Teixeira

Vargas²,

Carmo³

et al. 1998

Respiration

View full text Add to dashboard Cite

The ideal pleural sclerosing agent should be easily administered, without significant side effects, inexpensive, and widely available. None of the agents presently used meets all of these criteria. Ethanolamine oleate (ETH) is a sclerosing agent used in the sclerotherapy treatment of varicose veins of the legs and esophagus. The objective of the present study was to assess the efficacy of ETH as a pleural sclerosant in rabbits. An additional objective was to assess if better results were obtained when dextrose 50% (D50) as opposed to saline was used as the diluent. Each group of 10 rabbits received a total volume of 2 ml intrapleurally. The eight treatments were as follows: (1) 2 ml saline; (2) 2 ml D50; (3) 25 mg ETH plus 1.5 ml saline; (4) 25 mg ETH plus 1.5 ml D50; (5) 50 mg ETH plus 1.0 ml saline; (6) 50 mg ETH plus 1 ml D50; (7) 75 mg ETH plus 0.5 ml D50, and (8) 100 mg ETH. The rabbits were sacrificed 28 days after the injection. The intrapleural instillation of ETH resulted in evident pleurodesis, which was dose-dependent; 100 mg ETH induced significantly (p < 0.05) more adhesions than did any other treatment. The selection of the diluent had no effect on the pleurodesis. The microscopic examination of the right visceral pleura showed that the mean degree of fibrosis after 100 mg ETH was significantly (p < 0.05) greater than that after the other solutions. The mean degree of pleural inflammation, lung inflammation and lung fibrosis was minimal in all the groups. From this study we conclude that undiluted ETH produces pleurodesis in our experimental model. At the doses used, the pleurodesis was less than that produced after talc, tetracycline derivatives or silver nitrate in the same model.

show abstract

“…Right hemithoracal ribs were cut from sternal junction and full exposure of the pleural space was obtained. Macroscopic scoring of pleurodesis was performed by a surgeon and a pathologist who were blinded to the groups, according to the method described by Hurewitz et al [7] Scoring was as follows: grade 0= normal pleura; grade 1= few scattered adhesions; grade 2= generalized scattered adhesions; and grade 3= complete obliteration of the pleural space by adhesions.…”

Section: Methodsmentioning

confidence: 99%

Does medical ozone increase the pleurodesis effectiveness of autologous blood?

Yıldızhan¹

2016

Turk Gogus Kalp Dama

View full text Add to dashboard Cite

Histologic Changes of Doxycycline Pleurodesis in Rabbits

Cited by 79 publications

References 17 publications

Corynebacterium parvum versus tetracycline as pleural sclerosing agents in rabbits

Corynebacterium parvum versus tetracycline as pleural sclerosing agents in rabbits

Effectiveness of Ethanolamine Oleate as a Pleural Sclerosing Agent in Rabbits

Does medical ozone increase the pleurodesis effectiveness of autologous blood?

Contact Info

Product

Resources

About