Histologic changes in allergic and nonallergic wheals

Kline, B. S.; Cohen, Milton B.; Rudolph, J

doi:10.1016/s0021-8707(32)90148-8

Cited by 97 publications

(14 citation statements)

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“…Although the role of NCF in bronchial asthma is unclear, the factor may participate in the early recruitment of neutrophils characteristic of immediate hypersensitivity tissue reactions (28)(29)(30)(31)(32)(33). Infiltrating neutrophils, by releasing intracellular constituents, may contribute to bronchial hyperreactivity.…”

Section: Discussionmentioning

confidence: 99%

Identification and partial characterization of an exercise-induced neutrophil chemotactic factor in bronchial asthma.

Lee¹,

Nagy²,

Nagakura³

et al. 1982

J. Clin. Invest.

186

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A B S T R A C T A heat-stable neutrophil chemotactic factor (NCF) has been identified in the serum of 13 atopic asthmatic subjects after treadmill exercise. Peak activity was detected at 10 min and returned to prechallenge values by 1 h. No NCF activity was detected in the sera of three nonasthmatic atopic or four normal nonatopic individuals performing the same task. NCF produced by exercise (NCFEX) had a similar timecourse of release to NCF provoked by specific antigen (NCFAG). The appearance of circulating NCFEX and NCFAG closely paralleled the fall in peak expiratory flow rate/forced expiratory volume in 1 s (PEFR/ FEVI). Histamine challenge in atopic asthmatics at concentrations giving a comparable change in PEFR/ FEVy to that evoked by exercise or inhaled antigen was not associated with the appearance of circulating NCF. In seven subjects NCFEX release was inhibited by prior administration of disodium cromoglycate. NCFEX and NCFAG eluted as single peaks of activity when applied separately to columns of Sephadex G-200, and both were an estimated 750,000 daltons. NCFEX and NCFAG also eluted as single peaks of activity, at between 0.15 and 0.30 M NaCl, following anion exchange chromatography on DEAE-Sephacel (pH 7.8). The isoelectric points of NCFEX and NCFAG were virtually identical (between pH 6.0 and 6.5) as determined by chromatofocusing on Polybuffer Exchanger 94. The activities of NCFEx and NCFAG were substantially reduced, in both a time-and dose-dependent fashion, after incubation with trypsin and chymotrypsin. Partially purified NCFEX and NCFAG promoted both stimulated random migration (chemokinesis) as well as directional migration (chemotaxis).These experiments indicate that NCFEX and NCFAG might be identical substances and raise the possibility

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Section: Discussionmentioning

confidence: 99%

Identification and partial characterization of an exercise-induced neutrophil chemotactic factor in bronchial asthma.

Lee¹,

Nagy²,

Nagakura³

et al. 1982

J. Clin. Invest.

186

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“…However, the eosinophilic response to PAF does not approach the intensity of an allergic reaction (Hutson et al, 1988), which indicates an involvement of other mediators. Since the cellular response of sensitized animals (Samter, 1949;Sanjar et al, 1989) or man (Kline et al, 1932;Henocq & Vargaftig, 1988), to stimuli other than antigen, is qualitatively and quantitatively different from that of normal individuals, it is likely that sensitization in some way alters the response pattern. Recent studies may provide a molecular basis for such changed behaviour, since exposure of leucocytes to recombinant cytokines in vitro can induce a heightened responsiveness to activation stimuli (Owen et al, 1987;Dahinden et al, 1988).…”

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confidence: 99%

“…Discussion This study demonstrates that administration of recombinant human cytokines (rh-GMCSF or rh-IL3) to guinea-pigs can lead to changes in leucocyte populations in blood, bone marrow or airways, when doses higher than those normally used to treat patients with leukopenia are given. However, the capacity of rh-GMCSF to increase the eosinophilic response of the guinea-pig to aerosolised PAF is of greater importance, since such an enhanced reactivity to a variety of stimuli is a feature of sensitized guinea-pigs (Samter, 1949;Sanjar et al, 1989) or man (Kline et al, 1932;Henocq & Vargaftig, 1988). These observations imply that exposure to cytokines, such as GMCSF, during sensitization may be responsible for the exaggerated eosinophil response.…”

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confidence: 99%

Pretreatment with rh‐GMCSF, but not rh‐IL3, enhances PAF‐induced eosinophil accumulation in guinea‐pig airways

Sanjar¹,

Smith²,

Kings³

et al. 1990

British J Pharmacology

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Intraperitoneal injections of recombinant human granulocyte‐macrophage colony stimulating factor (rh‐GMCSF, 50 μg kg−1 daily) or interleukin‐3 (rh‐IL3, 50 μg kg−1 daily) for two days, induced an increase in the percentage of bone marrow and pulmonary airway eosinophils in the guinea‐pig. In addition, rh‐IL3‐treated animals exhibited an increase (21%) in blood neutrophils. Exposure of guinea‐pigs to an aerosol of platelet activating factor (PAF) gives rise to a selective pulmonary eosinophil accumulation, maximal at 48 h. The eosinophilic response to PAF was significantly enhanced in rh‐GMCSF‐treated guinea‐pigs but was suppressed in rh‐IL3‐treated animals.

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“…When lesions of urticaria pigmentosa were stroked they showed some degranulation of the mast cells with a slight influx of eosinophils [16], and in man marked local cosinophilia followed injection of histamine in the skin of atopic individuals, although this did not occur in those who were not atopic [17,18]. Marked local eosinophilia followed injection of histamine in horses [2,19], but this effect was not seen in mice [20,21], guinea pigs [22] or dogs [23].…”

Section: Discussionmentioning

confidence: 99%