Histologic and Immunohistochemical Analysis of Nine Endometrial Stromal Tumors

Immunohistochemical profiling of cytokeratin expression by endometrial stroma sarcoma

“…A survey of the literature for reports of CK expression in ESS shows relatively few published studies, and they reported a rather wide variation in the incidence of keratin expression by ESS (0%-50% in studies with ≥2cases) [3,7,9,[19][20][21][22][23][24][25][26][27][28][29][30][31][32]. Factors responsible for [3] 4/17 24 Sumathi et al [20] 6/19 32 Aubry et al [7] 6/16 38 Oliva et al [9] 5/10 50 Oliva et al [21] 2/4 50 Oliva et al [22] 2/6 33 Binder et al [23] 3/6 50 Franquemont et al [24] 2/12 17 Farhood et al [25] 9/23 39 Devaney et al [26] 0/9 0 Lillemoe et al [27] 8/26 31 Abrams et al [28] 1/2 50 Dabbs et al [29] 0/2 0 Suzuki et al [30] 0/2 0 Tosi et al [31] 0/7 0 Bonazzi del Poggetto C et al [32] 0/11 0 This study 8/17 47 Total 56/194 29% the differences in the proportion of cases that showed positive cytokeratin immunoreactivity in those studies include differences in fixatives, other fixative-related factors such as promptness and duration of specimen fixation, antibodies-related factors (epitopes and concentration), and differences in the immunohistochemical protocols such as antigen retrieval techniques [22,24]. The published reports of cytokeratin expression in ESS [3,7,9,[19][20]…”

Characterization of a newly established endometrial stromal sarcoma cell line

“…Similar ultrastructural findings were observed in the only other cell line established from an ESS with demonstration of irregular microvilli, tight junctions and primary cilia (Fresia et al, 1992). It appears that a biphasic phenotype with mesenchymal and epithelial differentiation characteristics is not uncommon in ESS and reflects the potential of neoplastic endometrial stromal cells to differentiate into various cell types (Binder et al, 1991;Farhood and Abrams, 1991). This is not unexpected, given the embryologic background of ESS with derivation from mesodermal pluripotential müllerian cells, which give rise to a variety of cell types found in normal tissues of the female genital tract.…”

“…Several cell lines from uterine carcinosarcomas have been established, providing in vitro models to study the mechanisms of heterogenous differentiation patterns in MMT (Ishiwata et al, 1981;Emoto et al 1992Emoto et al , 1993Gorai et al, 1993Gorai et al, , 1997. In ESS, there has also been evidence of biphasic differentiation, as suggested by the finding of a mixed immunophenotype in a considerable proportion of these tumors (Binder et al, 1991;Farhood and Abrams, 1991;Lillemoe et al, 1991). However, to date only one cell culture model has been described that allows the analysis of mechanisms associated with the phenomenon of biphasic differentiation in ESS (Fresia et al, 1992).…”

“…Both components in MMT presumably originate from the same cell. This is suggested by immunohistochemical studies showing coexpression of cytokeratin and vimentin in epithelial cells as well as in stromal cells of MMT (Ishiwata et al, 1981;Masuda et al, 1987;Emoto et al, 1992Emoto et al, , 1993.In ESS, there has also been evidence for a biphasic differentiation, as immunohistochemical studies have revealed that the tumor cells may be stained positively for epithelial cell markers (Binder et al, 1991;Farhood and Abrams, 1991). A mixed immunophenotype is not unexpected given the embryologic origin of ESS and is related to the inherent capacity of the stromal cells to differentiate into various cell types including epithelium.…”

Mixed tumors of the vagina: an immunohistochemical study of 13 cases with emphasis on the cell of origin and potential aid in differential diagnosis

“…[29][30][31][32][33] The largest immunohistochemical analysis of MTsV reported by Branton and Tavassoli 1 found intense reactivity with AE1/3 in nine out of 10 MTsV, while another tumor only showed sparse reactivity in the stromal-like component that was by far the predominant and in some cases the only component in these tumors. Similar results have been reported by others.…”