Histologic Analysis of the Influence of a Gelatin-based Scaffold in the Repair of Immature Dog Teeth Subjected to Regenerative Endodontic Treatment

Londero, Clacir de Lourdes Dotto; Pagliarin, Cláudia Medianeira Londero; Felippe, Mara Cristina Santos; Felippe, Wilson Tadeu; Danesi, Cristiane Cademartori; Barletta, Fernando Branco

doi:10.1016/j.joen.2015.01.033

Cited by 27 publications

(33 citation statements)

References 39 publications

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“…[10][11][12]14,15,17,21,22 Of these authors, only one provided the requested information, with the remainder either not having the data, or not replying.…”

Section: Resultsmentioning

confidence: 99%

“…Qualitative data regarding histological evaluation of dentin and intracanal hard tissue reported in the included studies followed previously published criteria for identification of dentin, cementum, bone, and periodontal ligament (PDL), 10,11,17,44 but were modified using the term "hard tissue" instead of "mineralized tissue. "…”

Section: Methodsmentioning

confidence: 99%

“…[n=NR] Zhu et al 13 [n=10C] Yoo et al 15 [n=16C] Londero et al 17 [n=17C] Torabinejad et al 19 [n=2T]…”

mentioning

confidence: 99%

“…This connective tissue ingrowth from periapical tissue was densely infiltrated with young fibroblasts and fibrocytes and well vascularized" 13 "A majority of teeth in each experimental group showed coronally proliferation granulation tissue" 14 "Vital pulplike tissue was more frequently observed in CM-treated teeth with abundant vascular supplies" 15 "pulplike tissue (including fibroblasts and blood vessels) in the root canal did not connect with the periodontium tissue in the apex" 16 "The new vital tissue formed in the root canal space showed histologic characteristics of fibrous connective tissue similar to periodontal ligament" 17 "ingrowth of soft tissue into the canals from the periapical tissues were observed in all revascularized teeth", "In six cases, the connective tissue appeared to be more "fibrous and continuous with the apical periodontal ligament" "Remnants of normal pulp tissue were observed on the canal wall in two revascularized teeth"…”

mentioning

confidence: 99%

“…However, animal studies and human case reports have shown that the newly developed tissues are often different from dentin and pulp parenchymal tissue. [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] The presence of a scaffold is considered to be an essential element for any regenerative treatment. Generally, a scaffold is a three-dimensional replica of the extracellular matrix which provides biological and mechanical support to the stem cells.…”

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confidence: 99%

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Histological assessment of regenerative endodontic treatment in animal studies with different scaffolds: A systematic review

Altaii

Richards

Rossi‐Fedele

2017

Dental Traumatology

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“…[10][11][12]14,15,17,21,22 Of these authors, only one provided the requested information, with the remainder either not having the data, or not replying.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…[n=NR] Zhu et al 13 [n=10C] Yoo et al 15 [n=16C] Londero et al 17 [n=17C] Torabinejad et al 19 [n=2T]…”

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Histological assessment of regenerative endodontic treatment in animal studies with different scaffolds: A systematic review

Altaii

Richards

Rossi‐Fedele

2017

Dental Traumatology

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Improving pulp revascularization outcomes with buccal fat autotransplantation

Khazaei

Khademi

Torabinejad

et al. 2020

J Tissue Eng Regen Med

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Several techniques have been introduced to improve the pulp revascularization outcomes. The use of the tissue graft can create more practical tissue regeneration, provide vascular supply, and enhance tissue healing. The aim of the present study was to investigate the histologic and molecular outcomes of pulp revascularization with buccal fat autotransplantation. Fifty‐six open apex roots from four dogs aged 4–6 months were randomly allocated to five groups of endodontic regeneration models: Group 1 (negative control, n = 4); Group 2 (control and without intervention, n = 4); Group 3 (blood clot, n = 16); Group 4 (buccal fat autotransplantation, n = 16); and Group 5 (blood clot plus buccal fat autotransplantation, n = 16). After 3 months, the extracted dog teeth were analyzed by histological and immunohistochemical techniques. Furthermore, real‐time quantitative polymerase chain reactions were implemented to assess the gene expression profiles of dentin sialophosphoprotein (DSPP), dentin matrix protein (DMP), collagen I (COL1), and alkaline phosphatase (ALP) on regenerated tissue in the root canals. There were no significant differences in the severity of inflammation and necrosis between intervention groups. Immunohistochemical analysis showed significant differences among the study groups in expression level of extracellular glycoproteins such as fibronectin, laminin, and tenascin C. Group 5 showed an increase in the expression of DMP1 and COL1 genes. The expression of DSPP gene increased significantly in Group 4. The expression of ALP gene increased significantly in Group 3. Using this procedure may open new fields of research for regenerative endodontic procedure in which tissue autotransplant, particularly adipose tissue, may improve the outcomes of pulp revascularization.

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