Histogenic analysis of chemically induced mixoploids in Spathiphyllum wallisii

Vanstechelman, Ives; Eeckhaut, Tom; Huylenbroeck, Johan Van; Labeke, Marie-Christine Van

doi:10.1007/s10681-010-0133-z

Cited by 12 publications

(5 citation statements)

References 30 publications

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“…According to the authors, many sectoral chimeras are not detected in the first analysis by flow cytometry. Plants of Spathiphyllum wallisii Regel, for instance, that were initially classified as tetraploids showed roots with diploid and/or mixoploid cells (Vanstechelman et al, 2010). Here, the synthetic tetraploids of L. alba presented sixty chromosomes in the metaphases confirming the ploidal level indicated by the flow cytometry analysis.…”

Section: Discussionsupporting

confidence: 70%

“…These results reveal that the response to the duplication process may vary among species, according to the methodology employed and the maintenance of the synthetic plants. Vanstechelman et al (2010) suggest that synthetic polyploid plants should be reanalyzed after the in vitro micropropagation procedure. According to the authors, many sectoral chimeras are not detected in the first analysis by flow cytometry.…”

Section: Discussionmentioning

confidence: 99%

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Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

et al. 2020

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Polyploidy is widely recognized as a major evolutionary force in plants and has been reported in the genus Lippia (Verbenaceae). Lippia alba, the most studied species, has been documented as a polyploid complex involving at least four ploidal levels. L. alba presents remarkable chemical and genetic variation and represents a model for understanding genome organization. Although the economic and medicinal importance of the species has been widely described, no established polyploid induction protocol has been reported so far. Here, we describe the production of synthetic polyploid plants of L. alba using colchicine. The ploidal levels were estimated by flow cytometry and chromosome counting. In addition, FISH and molecular markers approaches were used to confirm the stability of the synthetic polyploids. The major component of the essential oils was estimated by GCMS to compare with the natural individuals. Tetraploids and triploids were produced providing new opportunities for investigating medicinal, pharmacological, and economic applications as well as addressing intrinsic questions involved in the polyploidization process in tropical plants.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

et al. 2020

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“…However, to distinguish mixoploidy types, a combination of techniques is often needed. Vanstechelman et al (2010) proposed a combination of flow cytometry using leaves and roots together with a microscopical evaluation of cell nuclei to unravel the genomic composition of Spathiphyllum mixoploids initiated in vitro. The ultimate goal is to develop protocols for regeneration of solid polyploidized plants from mixoploid plants (Aleza et al 2009;De Schepper et al 2004;Roux et al 2001).…”

Section: Confirmation Of Polyploidymentioning

confidence: 99%

Mitotic chromosome doubling of plant tissues in vitro

Dhooghe¹,

Laere²,

Eeckhaut³

et al. 2010

Plant Cell Tiss Organ Cult

350

258

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In vitro chromosome doubling can be induced by several antimitotic agents. The most commonly used are colchicine, oryzalin and trifluralin. The process of induced chromosome doubling in vitro consists of a typical succession of sub-processes, including an induction phase and a confirmation protocol to measure the rate of success. The induction step depends on a large number of variables: media, antimitotic agents, explant types, exposure times and concentrations. Flow cytometry is the pre-eminent method for evaluation of the induced polyploidization. However, alternative confirmation methods, such as chromosome counts and morphological observations, are also used. Since polyploidization has many consequences for plant growth and development, chromosome doubling has been intensively studied over the years and has found its way to several applications in plant breeding. This review gives an overview of the common methods of chromosome doubling in vitro, the history of the technique, and progress made over the years. The applications of chromosome doubling in a broader context are also discussed.

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“…Based on the studies of Eeckhaut et al (2004) and Vanstechelman et al (2010), we have chosen three diploid genotypes, MS8 ('Speedy'), 6341 and 6526 and their stable tetraploid derivatives to use in the present study.…”

Section: Plant Materialsmentioning

confidence: 99%

Influence of ploidy level on morphology, growth and drought susceptibility in Spathiphyllum wallisii

Laere¹,

et al. 2010

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In this study, we analysed morphological, anatomical and physiological effects of polyploidisation in Spathiphyllum wallisii in order to evaluate possible interesting advantages of polyploids for ornamental breeding. Stomatal density was negatively correlated with increased ploidy level. Stomatal size increased in polyploids. Tetraploid Spathiphyllum plants had more ovate and thicker leaves. The inflorescence of tetraploids had a more ovate and thicker spathum, a more cylindrical spadix and a thicker but shorter flower stalk. Biomass production of the tetraploids was reduced, as expressed by lower total dry weights, and tetraploids produced fewer shoots and leaves compared with their diploid progenitors. Furthermore, tetraploid Spathiphyllum plants were more resistant to drought stress compared with diploid plants. After 15 days of drought stress, diploids showed symptoms of wilting, while the tetraploids showed almost no symptoms. Further, measurements of stomatal resistance, leaf water potential, relative water content and proline content indicated that the tetraploid genotypes were more resistant to drought stress compared with the diploids.

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Histogenic analysis of chemically induced mixoploids in Spathiphyllum wallisii

Cited by 12 publications

References 30 publications

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

Mitotic chromosome doubling of plant tissues in vitro

Influence of ploidy level on morphology, growth and drought susceptibility in Spathiphyllum wallisii

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