Histoenzymic studies on sublingual salivary gland of buffalo during prenatal development

Abstract

The histoenzymic studies on sublingual salivary gland of 15 buffalo foetuses ranging from 11 cm CVRL (78 days) to 100 cm CVRL (full term) were conducted. The foetuses were categorized into three groups based on their curved crown rump length (CVRL). At 11-19 cm CVRL (78-114 days), a weak activity of phosphatases and oxidoreductases was observed in the acinar cells and ductular epithelium. Between 28-37 cm CVRL (136-157 days), a weak to moderate diffused granular alkaline phosphatase (AKPase) activity was observed in the mucous acini whereas oxidoreductases showed moderate activity. The enzyme activity was progressively increased with advancement of the gestation period. AKPase activity was more in the lumen of acini and along the intercellular canaliculi at 42-100 cm of CVRL (168 days- full term). Large ducts exhibited strong activity for oxidoreductases indicating increased metabolic activity of cells.

Embryonic differentiation of intestine in Indian goats (Capra hircus)

“…The absence of argentaffine cells, paneth cells, intestinal glands and submucosal glands was in agreement with observations of Chima and Nwagbo (2009) in West African dwarf goat, Karadag et al (1994) in sheep. The lamina muscularis was not observed in early prenatal period of goat which was in full agreement with Chima and Nwagbo (2009) in West African dwarf goat, Karadag et al (1994) in sheep, Malhotra (2002) and Singh et al (2009) in buffalo feti. The propria submucosa comprised of mesenchymal cells, differentiating fibroblasts and small blood vessels was similar to evidences of Chima and Nwagbo (2009) in West African dwarf goat, Karadag et al (1994) in sheep.…”

“…Pig (Sangild et al, 2000), Buffalo (Singh et al, 2009) and in Camel (Bello et al, 2012), but very little systematic attention has been paid towards early prenatal development of the intestine in goats (Chima & Nwagbo, 2009;Gautam & Mishra, 2016;Raghav, 2016;Verma et al, 2020;Verma, Farooqui, Prakash, Pathak, Singh, Gupta, & Goyal, 2021;Verma, Farooqui, Prakash, Pathak, Singh, Gupta, Kumar, et al, 2021;Verma, Farooqui, Prakash, Pathak, Singh, Yadav, et al, 2021). Beside this, the information collected in the present study will also fulfil a gap in the scientific literature of prenatal development in goats.…”

“…In duodenum differentiating myocytes were noticed at 45th day which became differentiated into inner circular and outer longitudinal on 50th day of gestation. In buffalo feti the undifferentiated layer of muscle fibres was noticed at 47th day (Singh et al, 2009) and at 53rd day (Malhotra, 2002); this layer got differentiated into inner circular and outer longitudinal smooth muscles layer at 119th day (Singh et al, 2009) and at 117th day of gestation (Malhotra, 2002), respectively. In present study the inner circular smooth muscles layer was thicker than outer longitudinal layer as noticed by Ramkrishna and Tiwari (1979) in goat and Singh et al (2009) in buffalo feti.…”

“…The development of the intestine is important for the adult gastroenterologist to appreciate, because of the potential for these early life events to affect the responsiveness of the intestine to physiological or pathological challenges later in life (Drozdowski et al, 2010). Prenatal development of intestine has been studied in Sheep (Karadag et al, 1994), Pig (Sangild et al, 2000), Buffalo (Singh et al, 2009) and in Camel (Bello et al, 2012), but very little systematic attention has been paid towards early prenatal development of the intestine in goats (Chima & Nwagbo, 2009; Gautam & Mishra, 2016; Raghav, 2016; Verma et al, 2020; Verma, Farooqui, Prakash, Pathak, Singh, Gupta, & Goyal, 2021; Verma, Farooqui, Prakash, Pathak, Singh, Gupta, Kumar, et al, 2021; Verma, Farooqui, Prakash, Pathak, Singh, Yadav, et al, 2021). Beside this, the information collected in the present study will also fulfil a gap in the scientific literature of prenatal development in goats.…”

DISTRIBUSI KARBOHIDRAT NETRAL PADA LAMBUNG DEPAN SAPI SUMBA ONGOLE (Bos indicus)

“…Oleh karena itu, karbohidrat sebagai barier pertahanan penting dalam melindungi mukosa epitelium. Penelitian yang pernah dilakukan yaitu pada sapi aceh (Bos Indicus) (Agravion et al, 2018), sapi Yak (Bos grunniens) (Wang et al, 2014), kambing (Mahesh et al, 2014), domba (Neiva et al, 2006) dan kerbau (Singh et al, 2012), sedangkan penelitian pada sapi sumba ongole belum pernah dilaporkan.…”