Histochimie du neuropile du poulet pendant l'ontog�n�se ? hyperstri� vrai

Palladini, Giovanni; Alfei, L

doi:10.1007/bf00304254

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…The presence of an extracellular matrix in the mammalian central nervous system has been demonstrated with the aid of different techniques (Hess 1953, Uzman and Rumley 1958, Young and Abood 1960, Clausen and Hansen 1963, Bondareff 1966, Pease 1966, Rambourg and Leblond 1967, Palladini and Alfei 1968. Ultrastructurally the central fibres exhibit a node gap substance which is most abundant at the nodes of large fibres (Andres 1965, Peters 1966, Karlsson 1967, Conradi 1969.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural and light‐microscopic studies of the nodal region in large myelinated fibres of the adult feline spinal cord white matter

Hildebrand¹

1971

Acta Physiologica Scandinavica

118

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Section: Discussionmentioning

confidence: 99%

Ultrastructural and light‐microscopic studies of the nodal region in large myelinated fibres of the adult feline spinal cord white matter

Hildebrand¹

1971

Acta Physiologica Scandinavica

118

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“…Suffice it to say that the presence of hyaluronidase-sensitive mucopolysaccharides (glycosaminoglycans) in brain interstitium was shown in several studies (for references see Palladini and Alfei 1968;Deeler and Nakanishi 1983). The impact of this work, however, was negatively affected not only by the electron microscopic appearance of CNS tissue, but also by the belief that the cationic dyes used in these studies such as Alcian blue lacked specificity (Ripellino et al 1985).…”

Section: Introductionmentioning

confidence: 99%

Hyaluronic acid and hyaluronic acid-binding proteins in brain extracellular matrix

1993

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Hyaluronic acid (HA) plays the main structural role in the formation of brain extracellular matrix (ECM). The extracellular space appears empty by electron microscopy because HA is readily dissolved during the preparation of tissues for ultrastructural studies. The HA-binding proteins so far identified in brain ECM are versican, aggrecan and the glial HA-binding protein. Versican is a large fibroblast proteoglycan preferentially expressed in embryonic cartilage at the time of mesenchymal condensation. Glial HA-binding protein (GHAP) is probably a proteolytic product of versican corresponding to its HA-binding amino-terminal domain. It is mainly a white-matter protein, suggesting that the proteinase responsible for its cleavage from versican is normally activated in this location. Versican is found in both white matter and gray matter, where it forms pericellular coats around large neurons. Aggrecan, the aggregating proteoglycan of mature cartilage, co-localizes with versican in this location. In white matter, the localization of GHAP and versican is identical to that of the glial fibrillary acid protein, suggesting that both proteins are produced by astrocytes. An important difference between GHAP and versican is that GHAP but not versican is released from the tissues by hyaluronidase digestion, which suggests that versican is anchored to the cell membranes lining the extracellular space. GHAP was localized at the ultrastructural level in the granule cell layer of rat cerebellum, the only region of gray matter that is positive for GHAP in this species. Rats were perfused with aqueous fixatives containing cetylpyridinium chloride or tannic acid to prevent the solubilization of HA. GHAP is found throughout the extracellular space, the synaptic clefts being a notable exception. GHAP appears late in development, and the same is true for versican, the characteristic perineuronal coats first becoming apparent in the third postnatal week. It is suggested that a marked change occurs in the structure of brain ECM when HA-binding proteins first appear, and that the change is similar to that observed in prechondrogenic mesenchyme, i.e., reduction of the extracellular space and cell aggregation.

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Histochimie du neuropile du poulet pendant l'ontog�n�se ? hyperstri� vrai

Cited by 2 publications

References 29 publications

Ultrastructural and light‐microscopic studies of the nodal region in large myelinated fibres of the adult feline spinal cord white matter

Ultrastructural and light‐microscopic studies of the nodal region in large myelinated fibres of the adult feline spinal cord white matter

Hyaluronic acid and hyaluronic acid-binding proteins in brain extracellular matrix

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