Histochemistry of the prostate

Allsbrook, William C.; Simms, Wesley W.

doi:10.1016/0046-8177(92)90111-f

Cited by 38 publications

(12 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Normal prostatic tissue consists of two major compartments -the stroma and the epithelium [1,24,36]. Each of these compartments can be further subdivided into several predominant cell types.…”

Section: Significance Of the Igf System In The Normal Prostatementioning

confidence: 99%

The insulin-like growth factor system in the prostate

1995

View full text Add to dashboard Cite

Section: Significance Of the Igf System In The Normal Prostatementioning

confidence: 99%

The insulin-like growth factor system in the prostate

1995

View full text Add to dashboard Cite

“…Positive controls and a negative control with primary antibody omitted were run with each staining run. Tumor glands were identified using high molecular weight cytokeratin (HMWK Clone 34BE12; Dako Corporation) to distinguish acinar structures lacking HMWK+ basal cells [13] . Tissue microvessels including mainly capillaries and small venules with occasional arterioles were identified with the universal endothelial cell marker CD31 (Clone JC/70A; Dako).…”

Section: Methodsmentioning

confidence: 99%

Vascular Pericyte Density and Angiogenesis Associated with Adenocarcinoma of the Prostate

Killingsworth¹,

Wu²

2011

Pathobiology

View full text Add to dashboard Cite

Background/Aims: Angiogenesis facilitates metabolism, proliferation and metastasis of adenocarcinoma cells in the prostate, as without the development of new vasculature tumor growth cannot be sustained. However, angiogenesis is variable with the well-known phenomenon of vascular ‘hotspots’ seen associated with viable tumor cell mass. With the recent recognition of pericytes as molecular regulators of angiogenesis, we have examined the interaction of these cells in actively growing new vessels. Methods: Pericyte interactions with developing new vessels were examined using transmission electron microscopy. Pericyte distribution was mapped from α-SMA+ immunostained histological sections and quantified using image analysis. Data was obtained from peripheral and more central regions of 27 cases with Gleason scores of 4–9. Results: Pericyte numbers were increased around developing new vessel sprouts at sites of luminal maturation. Numbers were reduced around the actively growing tips of migrating endothelial cells and functional new vessels. Tumor regions internal to a 500-µm peripheral band showed higher microvessel pericyte density than the peripheral region. Conclusion: Pericytes were found to be key cellular components of developing new vessels in adenocarcinoma of the prostate. Their numbers increased at sites of luminal maturation with these cells displaying an activated phenotype different to quiescent pericytes. Increased pericyte density was found internal to the peripheral region, suggesting more mature vessels lie more centrally.

show abstract

“…As with other cancers, a progression from well-or intermediately differentiated tumours towards highgrade (HG), less well-differentiated ones is expected to occur at least in a subset of prostatic carcinomas. In the prostate, this dedifferentiation is accompanied by the occurrence of neuroendocrine tumour cells and a loss of PSA expression (Allsbrook et al 1992;Helpap et al 2002). Some prostate cancers, however, already have to be classified as HG tumours at the time of diagnosis.…”

Section: Introductionmentioning

confidence: 99%